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Animal Cell Line Solution
As a provider of products and scientific research services in the field of animal cells, BioVenic offers a range of comprehensive and professional customized solutions for the development, cultivation, characterization, identification, and rescue of animal cell lines. Leveraging advanced technology platforms, we facilitate genetic modification and immortalization of target cell lines, as well as provide a full spectrum of omics detection and analysis services for cell lines, catering to the requirements of scientific research projects involving animal cell lines. Our commitment lies in ensuring that your scientific research project commences with the right cell line, ensuring easy maintenance of cell viability and survival status, and yielding reliable and consistent research outcomes.
Fig.1 Morphology of monolayer cultures of bovine adipose-derived stem cells (bASC), passage 3, and 3T3-L1 murine preadipocyte cell line used as a reference cell line for adipogenic differentiation.1,2
Comparison of Animal Primary Cells and Cell Lines
| Characteristics | Primary Cell | Cell Line |
| Proliferation | Weak | Strong |
| Reproduction | Generally, within 10 generations | About 50 generations or more |
| Genetic Integrity | Not changed | Changed |
| Biological Properties | Close to in vivo cell physiology | Deviate with the number of divisions |
| Cultivation | Relatively difficult | Easy |
Cell Line Development
BioVenic specializes in designing and implementing customized solutions for cell line development. Our expert technical team addresses customer challenges at the root. Our comprehensive services encompass cell isolation, culture, characterization, expansion, transformation, and the establishment of target cell lines, effectively resolving all associated scientific issues in a single engagement. We create stable and immortalized cell lines tailored to meet customers' requirements for personalized cell lines in the absence of existing mature products.
- Tumor Cell Lines
- Immortalized Cell Lines
- Reporter Cell Lines
- Knockout Cell Lines
- Knock-in Cell Lines
- Overexpression Cell Lines
Cell Line Culture Development
BioVenic offers comprehensive services for developing animal cell lines in vitro. We utilize a robust quality control system to guarantee service excellence. With our extensive knowledge and experience in cell culture conditions and parameters across various species and sources, we customize cell line culture solutions to align with customers' specific project requirements and research objectives. Our tailored solutions are crafted to fully address a diverse array of cell culture needs.
Cell Line Characterization
BioVenic has established a comprehensive cell characterization consortium to offer diverse animal cell line characterization solutions or to tailor proprietary characterization systems for evaluating crucial parameters such as cell line identity, purity, genetic stability, and tumorigenicity. Animal cell line characterization entails measuring and assessing the traits and functionality of a cell line to confirm its identity, purity, and appropriateness for a particular use. This process guarantees that the cell line is genuinely sourced and has not been compromised by cross-contamination or external influences.
- Functional Analysis
- Teratoma Analysis
- Karyotype Analysis
- Signature Sequence Detection
- RT-PCR / qRT-PCR
- Transcriptome Sequencing
- MicroRNA Analysis
- Microbiological Testing
- Immunocytochemistry Analysis
- Proteome Analysis
- Methylome Analysis
- Gene Expression Analysis
Cell Line Identification
BioVenic provides cell line authentication services to researchers to prevent cross-contamination or misidentification of cells in research. These errors can result in inaccurate, unreliable, and irreproducible data, potentially leading to disastrous consequences for cell-related research. Rigorous and thoughtful authentication of animal cell lines ensures scientific integrity and reproducibility of experimental results. It is crucial for identifying and validating model cells used in scientific research and veterinary drug development. Our team of experts utilizes multiplex PCR detection, deep sequencing, DNA barcoding, RNA-seq, and other detection technologies to identify researchers' animal cell lines.
Cell Line Rescue
BioVenic offers professional services for rescuing animal cell lines to address issues such as loss of cell viability and contamination that may occur during repeated in vitro culture and passaging. We develop and implement tailored solutions to identify and resolve the root causes, thoroughly verifying them before delivery to guarantee the viability, passaging, and functionality of animal cell lines.
| Challenges | Causes |
| Misidentification | Cell line cross-contamination and misidentification can occur due to unclear labeling and record-keeping in the laboratory, aggressive cell line contamination, and the inadvertent combination of different cell lines in the same study. |
| Cell Death | Microbial contamination and improper handling, dissociation, and cryopreservation can result in cell death during culture. Excessive environmental stress can also lead to similar outcomes. |
| Precipitate | Precipitation can result from improper medium formulation and contamination. |
| Aggregation / Detachment | Detachment of adherent cells or aggregation of suspension cells is abnormal and indicates poor cell growth. |
| Microbial Contamination | Cell culture media and culture conditions provide an ideal environment for cells and bacterial, fungal, and viral contaminants. Common microbial contaminants cannot be detected visually. |
| Poor Growth | Media conditions and quality, supplement quality and suitability for use, as well as inaccurate cell counts during freezing or passaging, can result in cells failing to reach confluence and growing poorly in culture. |
Why Choose Us?
BioVenic has been deeply involved in the field of animal cells for many years and has a rich experience and deep professional background in various cell line products and related services.
BioVenic equips the animal cell culture platform with strict quality control and contamination prevention and control solutions to ensure the quality of each project service.
BioVenic offers a range of options for each service direction and is dedicated to fulfilling customers' diverse personalized project requirements and research objectives.
BioVenic offers comprehensive scientific research services related to animal cell lines, addressing multiple challenges encountered by researchers in this field. Leveraging our expertise in providing holistic solutions for animal molecular biology and cell biology, we facilitate rapid or in-depth characterization and identification of animal cell lines, along with the establishment of diverse stable cell lines. If your research is hindered by issues related to animal cell lines, don't hesitate to contact us to discuss with an expert.
References
- Image retrieved from Figure 2, Dzięgelewska-Sokołowska et al., 2023, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). The original image was modified by extracting and using only part (A), and the title was changed to "Morphology of monolayer cultures of bovine adipose-derived stem cells (bASC), passage 3, and 3T3-L1 murine preadipocyte cell line used as a reference cell line for adipogenic differentiation."
- Dzięgelewska-Sokołowska, Żaneta et al. "Adipocyte-Derived Paracrine Factors Regulate the In Vitro Development of Bovine Mammary Epithelial Cells." International journal of molecular sciences vol. 24,17 13348. 28 Aug. 2023.