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Animal Cell Proliferation Assay
Animal cell proliferation is one of the vital physiological functions of living cells, a crucial life characteristic of organisms, and the basis for animal growth, development, reproduction, and inheritance. The ability to detect animal cell proliferation is one of the key indicators for evaluating cell metabolism, physiology, pathological conditions, cell activity, cytotoxicity, and the effects of veterinary drugs. BioVenic's animal cell proliferation assay is designed to quantify the relative rate of animal cell division using specialized immunohistochemical staining technology to detect the proliferation process. Our cell proliferation ability assay starts from several closely related perspectives, including cell metabolic activity, protein expression related to cell proliferation, ATP concentration, etc. We develop various assay solutions to fully meet different project needs.
Fig.1 Cell Division and Proliferation.
Cellular Metabolic Activity Detection
BioVenic reduces the incubated substrate based on the metabolic capacity of the animal cells. It uses a spectrophotometer or microplate reader to detect the absorbance, which indirectly measures the number of viable cells and evaluates cell proliferation. This type of detection technology-based solution is widely applicable to various animal cell proliferation detection as well as other types of cell detection, including cytokine activity detection, cytotoxicity detection, and drug screening.
Cellular DNA Synthesis Detection
BioVenic has introduced a highly accurate solution for detecting cell proliferation by directly measuring the DNA synthesis content of animal cells to assess their proliferation ability. During the process of cell proliferation, one of the most significant changes is the doubling of DNA. Our technicians detect changes in the amount of DNA, determine variations in cell number, and perform cell proliferation tests, including isotope-labeled DNA, BrdU, and EdU detection methods.
ATP Content Detection
BioVenic uses the linear relationship between ATP concentration measured in cells and cell number to assess cell proliferation by detecting ATP. ATP is the primary source of energy for animal cells and its levels are closely linked to the cell cycle and physiological state. Dead or dying cells contain almost no ATP. Our service uses a standard reporter gene to detect ATP by bioluminescence. Its luminescence intensity is proportional to the intracellular ATP concentration, making it suitable for researchers requiring high-throughput detection and screening of cell proliferation.
Overview of Animal Cell Proliferation Detection Methods
Applications of Animal Cell Proliferation Assays
| Animal Health Analysis | Diseased cells exhibit different behaviors than normal healthy cells, including proliferation rates. By measuring cell proliferation in different cell populations, it is possible to gain information about their disease status and thus their overall health. |
| Cell Engineering Downstream Assessment | Cells or cell lines created by gene editing require thorough characterization, with cell proliferation being one of the critical parameters. |
| Veterinary Drug Screening and Evaluation | Determining the effect of drug molecules on cell proliferation after acting on specific cell types is also used to assess the toxicity of compounds to cells. It is the basic reference data and evaluation standard for veterinary drug screening. |
Why Choose Us?
A variety of detection methods can be flexibly selected according to project needs.
Combined with cell-based detection, a comprehensive analysis report is provided.
Highly sensitive and reproducible solutions ensure accurate scientific results.
BioVenic offers a wide range of assays and solutions for the analysis of cell proliferation in various applications such as monolayer cell cultures, 3D microtissues, primary cells, stem cells, and other animal cells. We use colorimetric, fluorescent, and bioluminescent detection methods to thoroughly evaluate cell proliferation capabilities. To meet dynamic research needs, our real-time live cell assays offer continuous monitoring, recording data sets at multiple points over time and providing comprehensive analysis. Our endpoint assays provide high-throughput capabilities for scientific users to efficiently assess cell health. If you are looking for a partner for animal cell-based testing and analysis, please contact us!