Animal Cell Viability Assay

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Animal Cell Viability Assay

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Overview of Animal Cell Viability Detection Methods Animal Cell Metabolism Detection Animal Cell Proliferation Detection Live Cell Imaging Technology for Animal Cell Viability Why Choose Us?

In biological experiments, it is essential to measure cell viability to assess the effect of a specific stimulus or drug on indicators such as sudden cell death, proliferation, growth inhibition, or toxicity. Cell analysis methods, such as PCR and antibody IHC staining for activity detection, have limitations because they can only reflect cell behavior at a specific point in time. Real-time cell analysis methods often lead to numerous new biological discoveries, making live cell analysis technology increasingly important in the scientific research process.

BioVenic has developed a wide range of research platforms for testing animal cell viability. These platforms help researchers measure changes in cell viability, assess cell health, and determine cytotoxicity. The solutions we develop for our customers consistently exhibit high dynamic range and low variability. We are committed to improving performance through technological advancements to ensure reliable detection capabilities and reproducibility of results.

Overview of Animal Cell Viability Detection Methods

Methods	Angles	Categories	Principles
MTT	Metabolism and Proliferation	Tetrazolium Salt Reduction	Succinate dehydrogenase in the mitochondria of living cells can reduce MTT to a water-insoluble blue-violet crystalline formazan, which is then deposited in the cells. This phenomenon does not occur in dead cells. After dissolving the formazan deposited in the cells with DMSO, use a microplate reader to measure the absorbance and calculate the number of viable cells.
XTT	Metabolism and Proliferation	Tetrazolium Salt Reduction	Use XTT to react with succinate dehydrogenase in the mitochondria of living cells and use a microplate reader to measure the absorbance of the product. The reduction product of the XTT method is a water-soluble orange-yellow formazan that allows direct measurement of light absorbance without the need for DMSO dissolution.
WST-1	Proliferation	Tetrazolium Salt Reduction	In the presence of electron coupling reagents, some dehydrogenases in the mitochondria reduce it to produce orange-yellow formazan. Formazan produced by the WST-1 method is water soluble, eliminating the need for subsequent dissolution steps and reducing errors.
CCK-8	Proliferation	Tetrazolium Salt Reduction	Highly sensitive WST-8-based assays for cell proliferation and cytotoxicity. Formazan produced by the CCK-8 method is water soluble and non-toxic to cells.
BrdU	Proliferation	DNA Synthesis Assay	Thymine derivatives can be used to label newly synthesized DNA in living cells. It can determine the type of proliferating cells, and their proliferation rate, and reflect cell viability.
EdU	Proliferation	DNA Synthesis Assay	It is incorporated into replicating DNA in place of thymine (T) and can be used to label newly synthesized DNA in living cells. It can determine the type of proliferating cells, and their proliferation rate, and reflect cell viability.
Alamar-Blue	Metabolism	Resazurin Reduction	The ready-to-use resazurin-based assay generates changes in brightness and fluorescence signals based on metabolic activity. A safe, non-toxic indigo dye is used to measure the metabolic activity of cells and assess their health and vitality.
ATP Assay	Metabolism	Luminescence	Exogenous firefly luciferin/luciferase is used to oxidize intracellular ATP to generate photons. The ATP content is detected by monitoring the luminescence to analyze cell activity.
Live Cell Fluorescent Dyes	Metabolism	Luminescence	Reactive dyes such as propidium iodide (PI) and calcein AM diffuse into cells and are hydrolyzed by intracellular non-specific esterases to produce fluorescence. Fluorescent products are produced and accumulated only in cells with intact cell membranes and active esterase activity. Used to monitor cell death (necrosis) and differentiate between live and dead cells.

Animal Cell Viability Assay Services

BioVenic masters a variety of cutting-edge cell detection technologies and develops detection solutions for ongoing research in various fields such as animal medical research and veterinary drug development. This provides unique insights into cell health, function and response to external stimuli. We offer customers a variety of cell viability assays to assess the health status of cells from different perspectives.

Animal Cell Metabolism Detection

BioVenic offers a range of cell viability assessment methods based on the detection of the metabolic activity of animal cells. The total metabolic activity of a cell or the metabolic activity of key pathways is sufficient to measure cell viability. Our MTT and XTT assays focus on mitochondria, the metabolic center of cells. They react with succinate dehydrogenase in the mitochondria of living cells to detect dynamic changes in cell activity by measuring changes in absorbance.

Animal Cell Proliferation Detection

BioVenic has introduced a comprehensive range of detection methods for the division and proliferation of animal cells. By detecting the proliferation of cell groups and individual cells, it can reflect the vitality of living cells. Detection methods based on cell activity mainly include MTT, WST-1, CCK-8, and BrdU. The BrdU method directly detects DNA synthesis in cells to measure cell proliferation, making it the optimal choice for obtaining accurate detection results.

Live Cell Imaging Technology for Animal Cell Viability

BioVenic's live cell microscopy imaging technology is a cutting-edge scientific research tool for animal cell biology research. Live cell imaging services enable the observation of the dynamics and interactions of specific biomolecules within animal cells over an extended period. Our R&D team utilizes various technological innovations to enhance the practical application of live cell imaging in animals. These include the creation of fluorescent proteins for gene expression and specific markers, as well as the use of genome engineering tools such as CRISPR to improve fluorescent reporters for protein labeling. We also support label-free cell imaging to meet the diverse needs of our customers and to reduce the toxicity of fluorescent dyes to cells. BioVenic uses time-lapse microscopy and advanced image analysis algorithms to provide non-invasive, real-time insights into animal cells.

Why Choose Us?

Comprehensive Assessment

Comprehensive detection and analysis of animal cell viability from multiple perspectives.

Diversified Testing

A variety of detection methods are available to meet the design requirements of different projects.

One-stop Service

One-stop analysis of animal cells includes assessments of cell viability, metabolism, proliferation, and cytotoxicity.

BioVenic assesses cell viability by measuring critical information about the health of animal cells, enabling researchers to determine whether target animal cells or cell populations can carry out essential biological processes. Dynamic monitoring of animal cell viability levels can help determine the success of animal cell-based applications. We offer scientific researchers a wide range of animal cell viability testing solutions to thoroughly and efficiently evaluate the effects of specific stimuli or drugs on cell survival, proliferation, growth inhibition, or toxicity. If you have a scientific research need related to animal cell viability detection, please contact us to discuss your project.