Animal Embryonic Stem Cell Solution

Animal embryonic stem cells (ESCs) are isolated from the inner cell mass of the blastocyst stage. Scientists continue to strive to effectively obtain ESCs from animal species and perform in vitro research, but it is more challenging than with other stem cells. BioVenic offers a range of services for animal embryonic stem cells, including separation and characterization, stem cell engineering, targeted optimization, and culture system development, to address ESC-related issues in customer projects in a personalized and precise manner. The goal is to advance research to improve animal health, animal production, and environmental stress. Our expert team utilizes various advanced technology platforms such as omics analysis, genome modification, animal transgenics, and stem cell culture system development and design to ensure seamless implementation and efficient completion of comprehensive solutions for ESCs.

Fig.1 Acquisition and Differentiation of Mammalian Embryonic Stem Cells.^1,2

Animal ESC Culture Development

BioVenic has developed several standardized culture systems for animal ESCs, including feeder layer culture systems, feeder-free systems and serum-free systems. These systems are designed to support ESCs to further divide and proliferate in vitro while maintaining their undifferentiated state. We assist researchers in overcoming the challenges of ESC culture during projects and scientific research. In addition, we design and develop customized culture methods tailored to their experiments. Our services also include technical support to improve and optimize existing culture systems.

Animal ESC Engineering

BioVenic is dedicated to the in-depth analysis of the molecular mechanisms of self-renewal and pluripotency regulation of animal stem cells and has accumulated extensive project experience. Our team of experts provides customized engineering solutions for stem cell applications with a focus on stemness regulation and genetic modification. We integrate cell biology and multi-omics platforms to collect omics data of animal ESCs and systematically identify genes or regulatory pathways associated with their self-renewal and pluripotency through bioinformatics analysis. We use animal genome editing technology to provide experimental solutions for animal ESC genome modification and gene function research.

Animal ESC Directed Differentiation

BioVenic has mastered various technologies to achieve directed differentiation of animal ESCs into cell populations of different lineages in different germ layers, providing personalized, customized services to researchers worldwide. With a robust genome editing platform, we support in vitro genetic modification of ESCs to reveal the regulation and function of specific genes during differentiation and development. We carefully categorize solutions based on induced differentiation technology, enabling customers to easily understand and select the service direction that matches their project content.

Animal ESC Isolation and Characterization

BioVenic has developed a comprehensive separation and characterization service for animal ESCs to ensure that the isolated cells retain their potential for multidirectional differentiation and self-replication after in vitro passage. The cells are derived from the inner cell mass of fresh embryonic tissue from healthy animals, ensuring stability and vitality. Interfering cells are removed and a suitable feeder layer is carefully selected. We establish a stable in vitro culture system, perform functional characterization, assess multi-lineage differentiation ability, and perform morphological identification after separation. Our ESC separation and extraction technology has been refined over years of project experience. Skilled technicians oversee the process, which includes a rigorous verification process to ensure the high quality and reliability of the service.

Animal ESC Genome Modification and Transgenic Technology

BioVenic stays abreast of the latest research trends in animal stem cells, offers CRISPR/Cas9 engineering and transgenics for animal ESCs. With the CRISPR/Cas9 method, we can easily achieve knockout and knock-in in the ESC genome, establish specific disease models, or explore key targets in the regulation of pluripotency and differentiation of pluripotent stem cells. At the same time, we use transgenic technology to transfer key genes into ESCs to direct their differentiation. Our genome editing technology has undergone multiple optimizations to maximize efficiency and control homologous recombination. With extensive experience in animal stem cell gene editing and transgenics, BioVenic offers customized solutions for knockout, knock-in, point mutation, and overexpression of exogenous genes in the ESC genome, ensuring high quality customized ESCs for customers. Our laboratory maintains high transfection efficiency through a rigorous and standardized quality control system. We integrate monoclonal identification technology with high-throughput screening to ensure efficient screening and expansion of positive clones.

Animal ESC Applications

• Cell Therapy

ESCs have great potential for differentiation and proliferation, high regenerative capacity, and broad prospects for the treatment research of animal diseases. Establishing a stable ES cell line from livestock or companion animals is the first step in advancing stem cell therapy.

• Animal Models

ESCs from farm animals such as pigs, cattle, sheep, goats or horses are used to develop and validate animal models for the evaluation of stem cell therapies. The creation of animal disease models through embryonic stem cell engineering serves to increase the economic importance and application of animals in veterinary medicine.

• Animal Breeding

Cultivating a population with superior traits through ESCs is incomparable to traditional animal breeding methods in terms of time, manpower, and other resources.

Why Choose Us?

BioVenic has extensive experience in providing scientific research services in the field of animal stem cells, ensuring that customers can efficiently and reliably address issues related to embryonic stem cells in their research. Our customized all-in-one solution covers various aspects of animal pluripotent cells, saving scientists time and effort in researching ESC isolation, differentiation, characterization and in vitro application conditions. Please do not hesitate to contact us to discuss your animal ESC research plans with our experts.

References

1. Image retrieved from Figure 1 "Generation of embryonic stem cells." Abou-Saleh et al., 2018, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). The original title was changed to "Acquisition and Differentiation of Mammalian Embryonic Stem Cells."
2. Abou-Saleh, Haissam, et al. "The march of pluripotent stem cells in cardiovascular regenerative medicine." Stem cell research & therapy 9 (2018): 1-31.