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Animal Genome Genotyping by Microarray
Introduction of Microarray Technology
BioVenic specializes in analyzing animal genetics through microarray chip genotyping services. We use modern chip designs on high-throughput platforms to process a large amount of animal gene and genotype data simultaneously, giving you a precise and rapid understanding of animal genetic variation and gene function.
Introduction of Microarray Technology
Animal microarray technology is a high-throughput animal genotyping method that can detect and analyze thousands of genes or gene variant loci in a single experiment. Microarrays are often made of glass, silicon, or other substrates. Using the principle of complementary pairing, probes are employed to match up with the desired DNA (or RNA) in the sample. The labelled complex's signal intensity is then gauged through fluorescent labelling or other detection methods to identify and scrutinize the genes (or gene variant loci). The probe sequences are generally brief DNA fragments or oligonucleotides connected with the gene or locus of interest.
Animal Microarray Chips Classification
| Category | Types | Introduction |
| Based on the type of DNA added to the vector | cDNA-based microarray | Probes are created using the transcription products (cDNA) of recognized genes. |
| Oligonucleotide-based microarray | Probes are composed of oligonucleotide sequences. Oligo probes usually have 20-25 base sequences, which represent a particular area or site of the genome. | |
| Based on functional | Gene expression microarray | This tool captures and measures mRNA levels in a sample using fixed probes made of either oligonucleotides or cDNA. It is used to identify variations in gene expression across different conditions and to research gene regulatory networks, developmental processes, and disease mechanisms. |
| SNP microarray | Contains a lot of probes that can identify genetic differences between people. These differences can be used to analyze population structures, determine kinship, and research genetic diseases. | |
| CNV microarray | The CNV microarray can detect segmental duplications or deletions in the genome, which can be used to study genetic diseases, population genetics, and genome evolution. | |
| DNA methylation microarray | To research the function of DNA methylation in controlling gene expression, growth, illness incidence, etc. | |
| miRNA microarray | This allows the study of miRNA expression patterns, as well as miRNA and gene regulation, and disease-linked miRNAs. |
Fig.1 Schematic Diagram of the Principle of Animal Microarray Genotyping.
Animal Microarray Genotyping Workflow
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Sample Preparation
Extracting and amplifying total RNA or genomic DNA from the sample, then labelling or transcribing it into cDNA. -
Sample Labelling
Nucleic acids in the sample are labelled using fluorescent dyes or other detectable markers to measure signal intensity during the assay. -
Microarray Hybridization
The marked sample undergoes a hybridization reaction with the probe sequence on the microarray chip so that the target DNA in the sample specifically attaches to the probe. -
Washing
Nucleic acids that loosely attach to the chip are eliminated by washing the chip multiple times. -
Signal Measurement
The chip is scanned with the appropriate equipment to calculate the intensity of the fluorescence of the labelled complex or any other detection signals. -
Processing and Analyzing Data
The recorded signals get transformed into digital information to normalize data, analyze differences, clusters, and so on. This process discloses data relating to the sample's gene or locus expression or genotype.
Fig.2 Workflow of Animal Microarray Genotyping. 2
Analytical Services
Animal Gene Discovery
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Molecular Animal Breeding
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Animal Population Research
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Basic Analysis
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Advanced Analysis
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Sample Requirements
| Sample | Requirements |
| DNA | ≥ 1.0 µg, concentration: ≥ 50 ng/µL |
| Tissue | ≥ 200 mg |
| Blood | ≥ 2 mL |
| Cell | ≥ 1X107 |
Why Choose Us?
BioVenic focuses on animal genotyping service, choosing our microarray chip service, you will benefit from the following aspects:
High precision analysis. Ensure the high precision and reproducibility of the chip test, so that the test results between different batches can be effectively compared and analyzed.
High sensitivity analysis. The sensitivity of the test is very high and can detect the target gene as low as 1 copy in 1 cell, while the amount of sample required for the test is small.
High accuracy analysis. Accurately detects different target genes, different states of the same target gene and differences in one base.
A quick way of analyzing a vast number of genes simultaneously.
Rapid and fully automated analysis with efficient measurement speed and efficiency.
BioVenic is dedicated to offering top-notch animal microarray genotyping services. Our aim is to provide you with precise, efficient, and inclusive genetic information analysis, which will give robust backing to your research and decision-making. Our team is committed to delivering expert services for animal microarray design and animal genotyping analysis, and if you have any queries, please feel free to contact us.
References
- Karampetsou, Evangelia, et al. "Microarray Technology for the Diagnosis of fetal chromosomal aberrations: which platform should we use?." Journal of clinical medicine 3.2 (2014): 663-678.
- From Wikimedia: https://commons.wikimedia.org/wiki/File:Microarray_exp_horizontal.svg