This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Animal Induced Pluripotent Stem Cell Directed Differentiation
BioVenic has extensive project experience in controlling the direction of differentiation of animal stem cells and offers directed differentiation services for a variety of stem cells, including iPSCs. In recent years, iPSCs have received increasing attention, and with the introduction of cell therapy drugs, the market development potential is huge. Animal iPSCs are similar to embryonic stem cells. They support unlimited replication in vitro, making them suitable for large-scale culture and scientific research. In the development of veterinary cell therapy, iPSCs can be derived from the sick animal itself to reduce the problems caused by immune rejection. At the same time, because iPSCs are derived from adult cells, several ethical issues are avoided. BioVenic induces iPSCs to differentiate into target cells based on customer requirements by offering high quality customized solutions. The technical solution is relatively stable and ensures repeatability.
Fig.1 Main Differentiation of Companion Animal iPSCs.1,2
Animal iPSC Differentiation Services
BioVenic has developed a range of potent cytokines to facilitate the study of animal iPSC cells, providing a reliable method for targeted differentiation in scientific research. At the same time, we have focused on the superior performance of mesenchymal stem cells (MSC) differentiated from iPSCs. We also offer customized services for this technology in connection with customer projects.
iMSC Differentiation
BioVenic provides customized services for the development of mesenchymal stem cells using animal iPSCs. The induced mesenchymal stem cells (iMSCs) exhibit superior performance in terms of cell proliferation, expansion capacity, immunomodulatory properties, and effects of paracrine signaling and immunomodulation. Our unique induced differentiation program has been continuously optimized and improved to address the shortcomings of traditional methods, such as complex induction processes, long induction times and low induction efficiency. This provides a solid foundation for sustainable development, quality improvement, and increased efficiency.
Directed Differentiation
BioVenic uses mechanical and chemical stimulation along with transcription factors to direct animal iPSCs to differentiate into three germ layers, giving rise to liver, heart, neurons, bone, muscle cells and more. We excel at using transcription factors, adjusting ratios, and precisely controlling the direction of cell fate. At the same time, we also provide customers with cell culture media, supplements, bioactive small molecules, and growth factors that can control the fate of animal iPSC cells, as well as comprehensive culture program development services.
Common Cytokines Used in Animal iPSC Differentiation
| Cytokines | Differentiation | Function |
| Activin A, VEGF, BMP-4, EGF, FGF-2, FGF-4, FGF-7, FGF-10, FGF-17, HGF, IGF-1, PDGF-BB, Shh, Noggin, Wnt 3a. |
|
For endoderm lineage differentiation |
| TPO, VEGF, FGF-2, FGF-3, FGF-10, FGF-17, Flt3-Ligand, IL-3, IL-6, IL-11, BMP-4, DKK-1, Noggin, Activin A, BMP-2, PDGF-BB, SCF, TGF-β1, Wnt-5a. |
|
For mesodermal lineage differentiation |
| BMP-4, EGF, FGF-2, Noggin. |
|
For ectodermal lineage differentiation |
Why Choose Us?
Expertise in Stem Cell Field
BioVenic has long been committed to research in the field of animal stem cells and has professional technology and extensive experience in inducing the differentiation of iPSCs.
Integration of Technologies
BioVenic has developed a combination of state-of-the-art technology platforms closely related to the field of animal cells. These include 3D culture, cell reprogramming, culture system development and cell characterization.
Diversification of Solutions
BioVenic has developed specific processing strategies and comprehensive evaluation solutions for pluripotent stem cells derived from various species and tissues to meet the needs of researchers.
BioVenic has professional technology, knowledge, and rich practical experience in directing the cell fate of animal stem cells. Relying on advanced animal stem cell technology, we have extensively performed animal iPSC reprogramming, gene editing, and various cell-directed differentiation processes to provide customers with high-quality customized experimental services. We are adept at using a variety of characterization and verification methods to track the fate of cells. We ensure that the results of cell differentiation are carefully and reliably confirmed through standardized processes with multiple quality controls. If you are interested in our animal iPSC directed differentiation services, please contact us for exclusive solutions!
References
- Image retrieved from Figure 2 "Applications of induced pluripotent stem cells (iPSCs) in companion animals for disease modeling and therapy." Barrachina et al., 2023, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). The original title was changed to "Main Differentiation of Companion Animal iPSCs."
- Barrachina, Laura, et al. "Induced pluripotent stem cells in companion animals: how can we move the field forward?" Frontiers in Veterinary Science 10 (2023): 1176772.