Animal Microbiome Full-length 16S rRNA Sequencing and Analysis

16S rRNA gene sequencing allows for the identification and comparison of microorganisms present in animal samples without the need for culturing. The 16S rRNA gene is present in nearly all microorganisms and is highly conserved, making it an ideal target for identifying and characterizing these microorganisms. BioVenic has developed a robust working system for studying complex microbiomes in animals that are difficult or impossible to study using full-length 16S rRNA gene sequencing based on next-generation sequencing (NGS). The 16S rRNA NGS solution enables researchers to achieve genus-level sensitivity in metagenomic surveys of bacterial populations in animals. This technology allows for a thorough investigation of the crucial roles these microorganisms play in animal physiological metabolism and disease. At the same time, our customized service solutions also support the analysis of the microbial composition that exists in the animal's living environment and the impact of the environment on the animal's physiological state. We utilize high-throughput sequencing of 16S rRNA microbiome technology to analyze microbial genome data rapidly and precisely. This enables our customers to identify and comprehend the distinct microbial universe present in various animal samples.

Fig.1 Application of Microbiome 16S rRNA Sequencing to Monitor Bacterial Communities. ¹

Animal Microbiome Full-length 16S rRNA Sequencing Services

BioVenic has launched customized services for high-throughput sequencing of full-length 16s rRNA, specifically designed for microbiome detection in animal samples. This service allows for the simultaneous detection of microbial groups in the sample, as well as the determination of the composition of the microbial community and their relative abundance. We are committed to helping researchers solve scientific problems related to exploring the diversity of microorganisms in animals and understanding the connections between them.

• Sample Preparation and QC

BioVenic offers comprehensive sample collection and processing solutions for scientific researchers. These solutions aim to ensure sample integrity and minimize changes in 16S rRNA gene sequencing, which can occur as bacterial communities evolve and adapt over time. If you are interested in studying the microbial composition of a particular animal sample, our customized sample preparation plan includes determining the sample collection location and time, implementing post-processing procedures, and conducting microbial gene extraction and quality control. If you want to explore the role and composition of a wider range of animal microbiomes, we employ the method of merging homogeneous sample preparation. This helps to balance the differences between individual animals or groups and expand the population coverage of data reflection.

• PCR Amplification

BioVenic has established a comprehensive molecular biology technology platform that provides reliable technical support for the PCR step in animal microbiome analysis. Amplification of quality-controlled nucleic acids provides a solid foundation for sequencing and identifying bacterial community composition.

• Library Construction and Sequencing

Our technical team designs primers, PCR, and sequencing procedures for library preparation based on customers' specific experimental needs and sample types. We optimize the target number of reads for each sample and incorporate negative and positive controls to ensure a flawless solution. BioVenic leverages years of experience in molecular biology projects to minimize the occurrence of residues and cross-contamination between samples. We deliver comprehensive 16s rRNA detection results for animal microbiomes, which are invaluable for bioinformatics analysis.

• Data Analysis

BioVenic has developed a sophisticated analysis system for full-length sequencing data of animal microbiomes, specifically targeting the 16S rRNA gene. Through high-throughput data analysis and integration with bioinformatics, we provide customers with a comprehensive interpretation of complex data. Our data processing system can effectively identify contaminating sequences during the 16S rRNA amplification process and display the relative abundance of microorganisms in the sample using reliable and rigorous analysis methods.

Flow Chart of Animal Microbiome Full-length 16S rRNA Sequencing Solutions

Why Choose Us?

BioVenic offers comprehensive 16S sequencing services for the detection of microbial diversity in various animal samples. We ensure that all common bacterial clades are accurately identified at the species level, compensating for any incorrect matches or indistinguishable phylogenies that may arise from partial 16S sequencing. By developing customized project plans for our clients, tailored to their specific scientific research objectives, we enable users to effortlessly utilize comprehensive 16S sequencing for phylogeny and prokaryotic identification. Additionally, our services facilitate the exploration of animal-microorganism interactions. Whether you are facing a technical or design challenge that seems difficult to overcome, contact us and get your project moving.

References

1. Jahan, Nusrat A., et al. "Nanopore-based surveillance of zoonotic bacterial pathogens in farm-dwelling peridomestic rodents." pathogens 10.9 (2021): 1183.
2. Peng, Siyi, et al. "Full-length 16S rRNA gene sequencing and machine learning reveal the bacterial composition of inhalable particles from two different breeding stages in a piggery." Ecotoxicology and Environmental Safety 253 (2023): 114712.