CRISPR gRNA Vector Preparation in Precision Animal Breeding

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CRISPR gRNA Vector Preparation in Precision Animal Breeding

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The development of CRISPR technology has accelerated the pace of precision animal breeding, greatly simplifying gene editing operations. Expression vectors loaded with various components necessary for editing and reporter genes are the key and difficult points of the entire CRISPR technology. With rich experience in CRISPR projects, BioVenic provides customers a variety of research-level gRNA vector construction solutions for genome editing in farmed animals, which are highly matched to the scientific research needs for customers worldwide.

Fig.1 Schematic Diagram of the CRISPR Genome Editing System. (BioVenic Original)

Services of CRISPR gRNA Vector Preparation

gRNA Vector Design

BioVenic specializes in customizing gRNA vectors design for genome editing research according to different animal species and editing targets. Take advantage of our extensive and professional experience in animal breeding projects, we aim to find suitable editing vector for you.

gRNA Plasmid Preparation

BioVenic provides gRNA vector preparation services, which includes gRNA optimization and synthesis, vector structure selection and construction, and vector amplification and purification. We have established a specialized library of commonly used CRISPR editing vector skeletons to meet customer needs and deliver research-grade, high-quality DNA vectors.

Target Cleavage Activity Assay

A variety of gRNAs correspond to a target gene sequence simultaneously, but they do not result in the same editing efficiency and specificity. BioVenic supports both in vitro and in vivo vector editing efficiency assays to provide customers with more reliable activity evaluation result and reduce failure and cost consumption in subsequent experiments.

Quality Control

BioVenic performs routine QC on gRNA plasmid vector DNA, including DNA quantification, concentration detection, restriction enzyme digestion, sequencing, purification, etc. We also perform it on the functionality of the gRNA vector to verify whether it has the correct editing effect on the genome target of farmed animals.

Why Choose Us?

The icon of the target.

Construction of gRNA Vectors Focusing on Animal Genome Editing

Based on our expertise in professional technical services in the field of CRISPR and animal precision breeding, we specialize in customizing plans for the vector requirements in genome editing projects for farmed animals. The vector structure, skeleton selection, and sequence optimization in each plan are highly adapted to the animal genome editing.

The icon of the target.

Efficient Editing Relying on Multi-locus Design

For the target gene, we adopt the design of multi-target editing window, and edit from multiple sites at the same time, ensuring the efficient and error-free completion of the overall project goals.

BioVenic has successfully established a mature and professional plasmid vector construction and quality control system. In addition, we have combined it with the CRISPR technical team in the direction of animal precision breeding, to launch complete gRNA vector preparation service customization for scientific research customers worldwide. Throughout the entire project, we provide technical support and assistance, helping you overcome any obstacles that may arise during your scientific research. Please feel free to contact us to customize your exclusive service for easy genome editing of farmed animal research program!