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Feed Postbiotics-Bacteriocins
Bacteriocins represent a diverse group of antimicrobial peptides or proteins produced by bacteria and archaea, displaying varied characteristics and specific immunity towards closely related bacterial strains. They demonstrate antibacterial properties and can be secreted by both Gram-positive and Gram-negative bacteria. Particularly noteworthy are bacteriocins sourced from lactic acid bacteria (LAB) among Gram-positive variants. BioVenic offers a range of solutions for advancing bacteriocins as feed additives in animal nutrition. Services include bacteriocin characterization, safety and efficacy studies conducted in vivo and in vitro, as well as production development.
Feed Postbiotics Bacteriocins Screening
Through our genetic testing and metabolite analysis technology, we can identify whether a specific probiotic strain harbors bacteriocin-related gene and if antibacterial active bacteriocins are present in the culture. This screening technology aids in developing bacteriocins from new probiotic strains as alternatives to antibiotics in animal feed.
Table. 1 Classification of bacteriocins and their major features1
| Class | Subclass | Example | Features |
|---|---|---|---|
| I (lantibiotic) | Ia (linear) | Nisin A |
|
| Ib (globular) | Suicin 3908 | ||
| Ic (multi-components) | Lacticin 3147 | ||
| II (non-lantibiotic) | IIa (pediocin-like) | Pediocin PA-1 |
|
| IIb (miscellaneous) | Aureocin A53 | ||
| IIc (multi-components) | Lactococcin G | ||
| III | IIIa (bacteriolytic) | Lysostaphin |
|
| IIIb (non-lytic) | Helveticin J | ||
| IV | None | Enterocin AS-48 |
|
Fig. 1 Mode action of bacteriocins2
Characterization of Feed Postbiotics Bacteriocins
Bacteriocins demonstrate antibacterial activity and a unique immunity mechanism towards closely associated bacterial strains. Our research platform is adept at delineating the physicochemical properties of bacteriocins, encompassing molecular size, thermal stability, pH resilience, and susceptibility to enzymes like proteases. Moreover, we evaluate the antimicrobial spectrum against fungi and common pathogenic bacteria. This detailed characterization aids in the development of feed postbiotic bacteriocins applicable across a wide array of scenarios in animal nutrition.
In Vivo and In Vitro Assessment of Bacteriocins as Feed Postbiotics
Our in vitro assessment solution for bacteriocins comprises processes such as simulated digestion and cellular models. By mimicking the conditions of the gastrointestinal tracts of various animals—adjusting factors like enzyme actions, pH levels, and temperatures—we assist researchers in probing the gastrointestinal tolerance of bacteriocins. This groundwork provides a theoretical underpinning for in vivo applications and facilitates the development of bacteriocin products with enhanced digestive tract compatibility. Additionally, our methodologies support in vitro studies involving probiotic strains that produce bacteriocins.
Leveraging our diverse animal models, we delve into the safety and efficacy profiles of bacteriocins in live animal settings. Our solutions enable extensive investigations into the impacts of bacteriocins from different sources, diverse dosages, and various combinations on specific animal species. Furthermore, our in vivo studies contribute to refining bacteriocin products concerning precise dosages, formulations, and additive types.
Mechanistic Exploration of Bacteriocins as Feed Postbiotics
A comprehensive understanding of the mechanisms underpinning bacteriocins can enable researchers to ascertain their production within specific probiotics. We offer an array of analysis technology platforms that cover genetic assays, metabolites, proteins, microbes, and more. Our assessments encompass both singular and omics analyses, facilitating a multifaceted exploration of how bacteriocins function as postbiotics in animal feed systems.
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Feed Bacteriocins Postbiotics Development
Our comprehensive solution offers all-in-one services for the development of feed bacteriocin products. Key components of our service package throughout the product development stage include strain selection or bioengineering, optimization of fermentation conditions, characterization of bacteriocins, safety and efficacy research, determination of suitable dosages and compatibility for feeding purposes, among others. Quality control measures are rigorously applied across the entire developmental process to ensure the production of superior products.
Applications of Feed Postbiotics Bacteriocins in Animals
Bacteriocins sourced from different origins exhibit varied structures and effects on animals. These compounds play crucial roles in regulating gastrointestinal microbiota, exerting anti-inflammatory properties, enhancing growth performance, and serving as viable antibiotic alternatives to combat pathogenic bacteria. Refer to Table. 2 for details on the impact of select bacteriocins on animals.
Table. 2 Some application effects of bacteriocins in livestock and companion animals
| Animal Types | Bacteriocins | Effects |
|---|---|---|
| Chickens | Pediocin A from Pediococcus pentosaceus | Supplementing with pediocin A enhanced broiler growth performance, particularly during challenges with Clostridium perfringens, and helped alleviate ADG depletion over a 42-day period. |
| Chickens | Nisin from Lactococcus lactis | Nisin, when included in the diet, operates similarly to salinomycin and can serve as a valuable dietary supplement for broiler chickens. |
| Chickens | Divercin AS7 from Carnobacterium divergens | Divercin-supplemented diets can influence the composition and activity of the microbiota within the gastrointestinal tract of broiler chickens, even in the lower regions. |
| Pigs | Colicin E1 from Escherichia coli | Including colicin E1 in the diet may offer an effective alternative to traditional antibiotics for weaning pigs, aiding in preventing postweaning diarrhea triggered by F18-positive enterotoxigenic E. coli. |
| Pigs | Pediocin A from Pediococcus pentosaceus | Pediocin A was able to directly reduce clostridia and indirectly impact coliform counts. Additionally, it helped diminish cecal fermentations. Its selective nature on certain microbial species controlled individual amino acid metabolism, reducing harmful secondary metabolites like p-cresol and enhancing desirable polyamines such as putrescine. |
| Common Carp | Nisin | Nisin exhibited notable anti-inflammatory effects on the intestinal tract of common carp, leading to a substantial increase in the expression levels of anti-inflammatory cytokines. Furthermore, supplementing with nisin enhanced specific digestive enzyme activities in the fish intestine. |
| Dogs | Nisin | Nisin, functioning as an antimicrobial agent, effectively reduced plaque buildup and gingivitis in beagle dogs. |
Feed Postbiotics Bacteriocins Development Workflow
Why Choose Us?
Our integrated suite of analysis services and cutting-edge in vivo and in vitro testing platforms empowers us to leverage advanced tools for the thorough study of bacteriocin characteristics.
Our animal platform offers a diverse range of animal models, facilitating detailed investigations into the effects of bacteriocins across various animal species.
Through our high-throughput omics platform encompassing genome, microbiome, proteome, metabolome analyses, we assist animal nutrition researchers in efficiently exploring the mechanism of action of bacteriocins.
BioVenic's service platform delivers comprehensive solutions for screening, characterization, in vivo and in vitro investigations, mechanism studies, and product development in leveraging bacteriocins as postbiotics in animal feed. Should you require assistance with the development of bacteriocins as feed postbiotics, please contact us. We eagerly anticipate learning about your research goals and offering viable service solutions.
References
- Ben Lagha, Amel, et al. "Antimicrobial potential of bacteriocins in poultry and swine production." Veterinary research 48 (2017): 1-12. Under Open Access license CC BY 4.0, without modification.
- Hernández-González, Juan Carlos, et al. "Bacteriocins from lactic acid bacteria. A powerful alternative as antimicrobials, probiotics, and immunomodulators in veterinary medicine." Animals 11.4 (2021): 979. Under Open Access license CC BY 4.0, without modification.