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Veterinary Fungal Pathogen 18S/ITS Amplicon Sequencing
In the field of veterinary science, accurate and rapid pathogen identification is crucial for effective disease research and development. Among the myriad of pathogens that affect animals, fungal pathogens present unique challenges due to their diverse and complex nature. Traditional diagnostic methods often fall short in identifying these pathogens promptly and accurately. This is where advanced molecular techniques, such as 18S/ITS amplicon sequencing, come into play. As a contract research organization (CRO) specializing in veterinary science, BioVenic is committed to employing cutting-edge technologies to enhance animal related research and development. Our expertise in sequencing and analyzing highly variable region of 18S rDNA or internal transcribed spacer of veterinary fungal pathogens offers insights into fungal pathogenesis and resistance.
Veterinary Fungal Pathogen 18S Amplicon Sequencing Services
18S rDNA is a DNA sequence encoding the small subunit rRNA of eukaryotic ribosomes, which can be a powerful tool for the identification and classification of eukaryotic organisms, including fungi. This method targets the highly conserved 18S ribosomal RNA gene, providing a reliable means as a molecular marker to differentiate between various fungal species and phylogeny.
- High Sensitivity and Specificity: The conserved nature of the 18S rRNA gene allows for the detection of a broad range of fungal species, including those that are difficult to culture.
- Comprehensive Database Comparison: Sequencing results are compared against extensive databases, ensuring accurate identification and classification.
- Phylogenetic Analysis: Helps in understanding the evolutionary relationships between different fungal species, which can be crucial for epidemiological studies.
Veterinary Fungal Pathogen ITS Amplicon Sequencing Services
The Internal Transcribed Spacer (ITS) regions, ITS1 (350 bp) and ITS2 (400 bp), located between the 18S, 5.8S and 28S rRNA genes, are highly variable and provides species-level resolution, making it the gold standard for fungal identification.
Fig.1 Sketch of the rDNA operon. 1
- High Resolution: ITS regions offer greater variability than the 18S rRNA gene, allowing for more precise species-level identification.
- Species-Level Differentiation: Capable of distinguishing closely related fungal species that may not be differentiated by 18S rRNA sequencing.
- Extensive Reference Databases: Utilizes comprehensive ITS sequence databases for accurate identification.
Comparison of Sequencing Methods
| 18S Amplicon Sequencing | ITS Amplicon Sequencing | |
| Variability | The 18S rRNA genes are relatively conserved and are suitable for a wider range of phylogenetic analyses. | ITS sequences are more variable compared to 18S rRNA regions. Since ITS sequences are more variable among species, they have higher resolution in species identification and classification. |
| Species Identification | 18S rRNA sequencing is usually used for more macroscopic classification and phylogenetic analysis. |
Due to the high variability of ITS sequences, they are more commonly used in species identification. ITS sequencing can provide more information to help distinguish between different species and subspecies. |
| Application | 18S rRNA sequencing is more commonly used in cross-species phylogenetic analyses, including comparisons between fungi and other organisms. | ITS sequencing is widely used in species identification and classification in the field of fungi, especially in strain identification and the study of fungal diversity in environmental samples. |
Technical Process of 18S/ITS Sequencing
Applications
Why Choose Us?
- We employ the latest next-generation sequencing (NGS) technologies to ensure high accuracy, sensitivity, and throughput. Our platforms are capable of handling diverse sample types and providing comprehensive data analysis.
- Our team of molecular biologists, bioinformaticians, and veterinary pathologists bring extensive expertise to every project.
- Our services are highly customizable for our clients. Clients will receive detailed reports that include raw sequencing data, sequence alignments, phylogenetic trees, and interpretive summaries.
BioVenic is dedicated to advancing veterinary fungal pathogen research and diagnostics through our comprehensive sequencing services. By leveraging 18S and ITS amplicon sequencing, we provide veterinary professionals and researchers with the tools they need to accurately identify and manage fungal infections in animals. Please contact us to learn more about our sequencing services, which support your veterinary research needs, improving animal health and welfare through precise and innovative molecular solutions.
Reference
- Kauserud, Håvard. "ITS alchemy: on the use of ITS as a DNA marker in fungal ecology." Fungal Ecology (2023): 101274.