In Vitro Animal Digestion Model Development

The use of an in vitro animal digestion model offers a valuable approach for investigating animal nutrition. These models replicate the conditions of the gastrointestinal tract by modifying the ionic strength, pH levels, and introducing enzymes and bile salts. These simulations are cost-effective, efficient, and eliminate ethical concerns associated with clinical trials. BioVenic specializes in the advancement of in vitro animal digestion models and provides comprehensive solutions to aid your animal nutrition research, ensuring a streamlined development process for our clients.

In Vitro Animal Digestion Model Optimization

To cater to specific research requirements, existing animal in vitro digestion models may not always suffice. We offer tailored optimization services for animal in vitro digestion models based on your animal species, experimental objectives, and any additional special requirements. Our service encompasses the optimization of the animal in vitro digestion solution composition, adjustment of digestion parameters and processes, and refinement of result analysis methods.

Fig. 1 Fish in vitro digestion protocol¹

In Vitro Animal Digestion Model Enzymatic Solutions

In in vitro digestion experiments, simulating the physiological environment necessitates the use of one or more enzymes. These enzymes may include mixed enzymes or individual ones like amylase, pepsin, trypsin, chymotrypsin, and others. At BioVenic, we not only provide enzyme products suitable for in vitro digestion models but also offer solutions for obtaining custom enzymatic combinations tailored to your specific project.

• Extraction and Purification of Digestive Enzymes

Digestive enzymes play a vital role in the digestive process and vary considerably across different animal species. To ensure more reliable experimental outcomes, it is necessary to extract digestive enzymes directly from the animal's digestive tract or organs. In addition to our existing range of animal-derived digestive enzymes, we also provide extraction and purification services, enabling you to obtain in vitro digestion models that closely mimic the physiological state of animals.

• Enzymatic Assays

To simulate the activity of digestive enzymes in the gastrointestinal tract and assess model feasibility, measuring enzyme activity is essential. We offer various enzymatic determination methods, including enzyme activity assessment, characterization, and kinetics testing. Our comprehensive enzymatic assays assist in addressing enzyme-related queries within in vitro digestion models.

• Novel Enzyme Development

Certain specialized animals or digestion models may require enzymes that are not readily available. In such cases, we can develop novel enzymes tailored to meet your specific needs. Our services cover enzyme design, expression, property determination, and large-scale production, all aimed at providing satisfactory solutions.

Artificial Gastrointestinal Fluid Development

Due to the diversity of animals, digestive environments vary in terms of pH, nutrient composition, ion composition, and more. To fulfill the diverse needs of animal nutrition researchers, we offer artificial gastrointestinal fluid development services. Our team develops customized products that precisely match your requirements, including composition, pH, viscosity, enzymes, stabilizers, and other customizable indicators.

In Vitro Animal Digestion Product Analysis

The in vitro digestion model serves as a valuable tool for evaluating nutritional digestion characteristics. Analyzing the products of in vitro digestion is a crucial step in such research. With our integrated analysis platform, we provide comprehensive analyses of digestibility, degradation rates, and products derived from various nutrients. Expect standardized testing and easily interpretable experimental results from our services.

Some of the testing items are as follows:

• Digestibility.
• Protein in vitro digestion products: amino acid composition, peptides.
• Carbohydrate in vitro digestion products: total sugar, reducing sugar, glucuronic acid, etc.
• Lipid in vitro digestion products: TAG, DAG, FFAs, etc.
• Structural analysis: scanning electron microscopy, laser particle size analyzer, etc.
• Other characteristics: antioxidant properties, etc.

In Vitro Animal Digestion Model Development Service Process

In Vitro Animal Digestion Model Applications

• Feed Development

The evaluation of nutrients within living organisms is typically done by conducting feeding trials on the specific animals of interest, where measurements of growth performance and fecal nutrient content are taken. While this approach delivers more precise and representative outcomes, it is time-consuming and costly. To address this, in vitro methods employing laboratory equipment have gained popularity for assessing digestibility by simulating the physiological processes of the animal's gastrointestinal tract. In vitro digestion model is now a useful tool for development of new feed and novel formulations.

• Feed Additive Development

The development of feed additives is beneficial for improving the growth performance and product quality of animals. The activity and availability of feed additives are the focus of attention in their development. The evaluation of feed additives through in vitro digestion models can study their tolerance to the digestive system environment and their metabolic mechanisms. Our in vitro animal digestion model development solution can provide valuable insights for the development of feed additives such as enzymes, probiotics, and prebiotics.

• Mechanism Studies

By simulating the physiological state of gastrointestinal tract in animals, we can study the mechanism and action of certain nutrients, which is beneficial for understanding the source, transformation process, and product type of a certain nutrient before conducting in vivo research. As a preliminary experiment for animal experiments, it provides valuable information for formal experiments, which is beneficial in reducing unnecessary human and material costs.

Why Choose Us?

Our goal is to solve the challenges you face in animal in vitro digestion through our animal in vitro fermentation model development service. With our in vitro animal digestion model optimization, in vitro animal digestion model enzyme solutions, artificial gastrointestinal fluid development and in vitro animal digestion product analysis services, you can explore the multiple applications of in vitro animal digestion models. Together, we aim to advance the fields of animal nutrition and feed development. If you are interested in our services, please contact us. We eagerly await your contact.

Reference

1. Wang, Ricky, et al. "In-vitro digestion models: a critical review for human and fish and a protocol for in-vitro digestion in fish." Bioengineered 12.1 (2021): 3040-3064.
   Under Open Access license CC BY 4.0, without modification.