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In Vitro Animal Fermentation Model Development
The gastrointestinal tract of animals serves as a primary location for digestion, absorption, and hosting a diverse microbial population. Microbial fermentation within this ecosystem yields crucial end products that greatly influence animal health. Traditionally, investigating animal microecology entails sacrificing live subjects for sample collection, posing limitations in reproducibility and ethical concerns. BioVenic's in vitro animal fermentation model development service introduces a novel method for gastrointestinal microbiota research, offering a sustainable alternative. This platform functions as a valuable tool for exploring prebiotic and probiotic development, as well as delving into the intricate mechanisms behind probiotic-nutrition-microbe interactions.
In Vitro Fermentation Model Development by Animals
Monogastric Animal In Vitro Fermentation Model Development
A monogastric animal possesses a single-chambered stomach. Common examples of monogastric animals are horses, pigs, dogs, and cats. Monogastric herbivores exhibit enhanced fiber digestion capabilities, attributed to their expansive cecum. With extensive experience in exploring monogastric animal fermentation physiology, BioVenic offers tailored in vitro fermentation model development solutions to animal nutrition researchers. Our specialized in vitro fermentation model for monogastric animals concentrates on simulating microbial fermentation processes within the large intestine and cecum, providing valuable insights for research purposes.
Ruminant Animal In Vitro Fermentation Model Development
Ruminant animals possess a complex four-chambered stomach. Common examples of ruminants include cattle, goats, and sheep. Exploring ruminant animal in vitro fermentation models holds promise for developing novel additives that can modulate microbial populations within the rumen. This approach aims to decrease greenhouse gas emissions and minimize energy and protein losses. Building on strong collaborations with both academic and industrial partners engaged in rumen in vitro fermentation studies, our technology platform offers a range of tailored solutions for ruminant animal in vitro fermentation model development. Find more detailed information through the following link.
In Vitro Fermentation Model Development by Model Types
In Vitro Batch Fermentation Models
The primary goal of in vitro batch fermentation models is to assess the fermentation capabilities of gastrointestinal ecosystems with a particular substrate. This process takes place in enclosed systems, mixing a defined amount of substrate with microbial inoculum and allowing them to ferment for a set duration.
- Advantages: Fast, inexpensive, easy to operate, reproducible, high throughput and automatable. It allows for the minimization of the required media volume and test compounds.
- Limitations: Limited in terms of experimental duration and the amount of substrate supply to avoid negative feedback mechanisms, highly dependent on the inoculation density.
In Vitro Continuous Fermentation Models
In vitro continuous fermentation models are specifically designed to facilitate a continuous and controlled fermentation process within a system. This model replicates the continuous flow of materials, enabling long-term experiments to access steady-state conditions.
- Advantages: Superior in modeling the dynamic nature of the gastrointestinal tract. Allows the adaptation of various parameters, including dilution rate, retention time, pH and temperature to meet and maintain optimal growth conditions. Facilitates studies on the modulation of microbial composition and activity.
- Limitations: expensive equipment, longer experimental period.
In Vitro Fermentation Model Development One-stop Solutions
Our one-stop solutions help in the design and construction of in vitro fermentation models that are suitable for different experimental purposes for researchers in the field of animal nutrition.
- Inoculum Preparation Solutions
The successful inoculation and colonization of in vitro fermentation systems are crucial factors that affect the reproducibility of studies, presenting a significant challenge for these models. Drawing upon our expertise in the development of animal in vitro fermentation models, we are dedicated to assisting you in preparing appropriate inoculums tailored to your specific requirements for animal in vitro fermentation research.
- Inoculum Obtaining Solutions
Animal in vitro fermentation models commonly utilize various types of inoculums, such as feces, cecal content, rumen content, and more. At our facility, we maintain strict supervision over the inoculant acquisition process. This includes implementing measures to minimize the use of antibiotics in donor animals, ensuring an anaerobic environment throughout the sampling process, minimizing external bacterial contamination, and employing appropriate pre-treatment methods for the collected samples. These meticulous steps help ensure the integrity and reliability of the inoculums used in our animal in vitro fermentation models.
- Inoculum Immobilization
BioVenic's immobilization technique helps to immobilize inoculums on different carriers to achieve high cell density, maintain bacterial diversity and high stability over extended in vitro fermentation periods.
- pH Solutions
We offer a complete solution for pH control throughout the fermentation process, including pH measurement of inoculants, pH adjustment of culture media, and pH monitoring during the fermentation process.
- Retention Time Solutions
Retention time is a crucial factor that significantly impacts the in vitro fermentation process. It is particularly influential in the degradation of proteins by gut bacteria and can influence the fermentation of carbohydrates. Our solution enables you to determine the specific retention time required for your study, allowing you to understand the effects of different retention times on your experimental outcomes. This information is invaluable for optimizing and adjusting your research to achieve desired results.
- Nutritive Medium Development
BioVenic offers nutritive medium development solutions according to your specific needs to mimic the physiological status of the colon, rumen and other gastrointestinal parts.
- In Vitro Fermentation Profile Analysis
BioVenic provides solutions for gas production (e.g. CH4 production) and fermentation rate analysis during animal in vitro fermentation to help researchers better understand the in vitro fermentation process. Our goal is to achieve accurate readings of in vitro gas production and to facilitate the collection of in vitro gas production samples.
- Animal In Vitro Fermentation Metabolite Analysis
BioVenic offers state-of-the-art analytical platforms that can identify and qualify metabolites during animal in vitro fermentation with high resolution. Our techniques include colorimetric assays, immunoassays, HPLC, GC, NMR, etc. By using our metabolomics and proteomics techniques, we can detect multiple metabolites in the same assay. Commonly tested fermentation metabolites include short chain fatty acids (SCFA), branched chain fatty acids (BCFA), ethanol, ammonia N, lactate, etc.
To learn more about services through the link below.
- Animal In Vitro Fermentation Microbial Analysis
We can not only analyze the level of marker microorganisms (e.g., probiotics, harmful bacteria) in the animal in vitro fermentation model for research needs, but also provide you with a comprehensive microbial picture through high-throughput microbiome detection and bioinformatics analysis. The information that can be obtained includes microbial composition, abundance, microecology analysis, correlation analysis, etc. to study the microbial functions and mechanisms of microbial action involved in animal in vitro fermentation models.
Why Choose Us?
With our focus on animal in vitro fermentation, we are able to provide the latest research methods and technologies.
We focus on the research of farm and companion animals and understand the difficulties you face in your research.
We have integrated multiple technology platforms for you to choose from, so you do not have to worry about every part of the experiment.
We take care of every aspect of your experimental design, execution and data analysis, providing you with timely and effective solutions.
As a provider of solutions in the field of animal nutrition, BioVenic is eager to establish friendly cooperative relationships with researchers in academia and industry. We are able to provide solutions for in vitro batch fermentation models and in vitro continuous fermentation models. For different animal species, including monogastric and ruminants. If you would like to learn more about our solutions for animal in vitro fermentation development, please contact us and our staff will respond promptly.