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Synthetic Peptide Subunit Veterinary Vaccine Development
Synthetic peptide subunit veterinary vaccines are not based on whole pathogens or parts of pathogens but are made by synthesizing linear antigenic epitopes with an immune response as peptides in vitro, coupling them to a carrier, and mixing them with adjuvants. Synthetic peptide vaccines have high safety and the ability to elicit specific immune responses. BioVenic provides one-stop synthetic peptide subunit veterinary vaccine development services, including antigenic epitope screening and characterization, peptide synthesis, and a variety of assessment services, and makes every effort to escort your vaccine development project.
Our Services
Epitope Recognition
Antigenic determinant clusters are parts of antigenic molecules called epitopes that can be recognized by animals' immune systems, especially by antibodies or T and B cells. Epitope recognition is considered important in synthetic peptide subunit veterinary vaccine design. BioVenic provides epitope prediction and recognition services, setting a solid foundation for your veterinary vaccine development.
| Epitope Prediction and Recognition Services | |
|---|---|
| Epitope Prediction and Recognition |
Screening candidate pathogen antigen sequences to identify potential T-cell and B-cell epitope clusters. Utilizing a combination of predictive conformational modeling and linear interaction mapping to map the epitopes. |
| Immunogenicity Assessment | Assessing the potential immunogenicity of each identified epitope cluster. |
| Comparison with Known Epitopes | Comparing each epitope cluster with other epitope clusters of known immunogenicity. |
| Biochemical Testing Methods |
Employing biochemical methods such as enzyme-linked immunosorbent assay (ELISA), immunoblotting, and fluorescence resonance energy transfer (FRET). Testing the specificity of binding to antibodies through these methods. |
| Identification of Antigen Epitopes | Using the results from biochemical tests to identify possible antigen epitopes. |
Peptide Synthesis
| Peptide Synthesis Services | Description |
|---|---|
| Solid Phase Synthesis | The starting amino acid is attached to the solid phase support and then the other amino acids are added sequentially. This method is suitable for synthesizing longer peptide chains. |
| Liquid Phase Synthesis | Amino acids are activated in the liquid phase and then linked to existing peptide chains. This method is more suitable for synthesizing shorter peptides. |
| Hybrid Synthesis | The method allows for the synthesis of short peptide fragments using a liquid-phase approach, followed by the application of the fragments to solid-phase synthesis. |
BioVenic has the ability to synthesize antigenic peptides by utilizing the advantages of solid phase synthesis (SPPS) or liquid phase synthesis (LPPS), flexibly choosing the appropriate synthesis method according to the type of peptide. BioVenic provides comprehensive peptide synthesis services, including but not limited to peptide synthesis, purification, characterization, conjugation and modification, and strict quality control, which meets all your peptide synthesis needs for veterinary vaccine development.
Fig.1 Schematic diagram of solid-phase peptide synthesis (SPPS) process.1
Carrier Coupling
Peptides alone are usually too small to elicit an adequate immune response, whereas carrier proteins with many antigenic epitopes facilitate the stimulation of helper T-cells, further inducing B-cell immunity and therefore response. Synthetic peptide subunit veterinary vaccines usually need to be coupled with appropriate carriers to enhance their immunogenicity, prolong their presence in the body, and facilitate an adequate response by the immune system. BioVenic is committed to providing support of the selection of carrier protein and coupling methods according to the characteristics of veterinary vaccines, immunogenicity requirements, and delivery routes. The following carrier proteins are commonly used for the development of peptide subunit veterinary vaccines.
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Advantages of Peptide Synthesis Services
Comprehensive Service Types
- Desalting.
- Aliquoting.
- Lyophilization.
- Solubility Test.
High Purity
- Purity determination with SDS-PAGE.
- Achieve 70% to >98% purity.
Customized Synthetic Length
- Up to 200 amino acids.
Tailored Quantity
- Milligrams to kilograms.
- From lab-scale to large-scale.
Peptide Conjugation and Modification
- Amino acid substitution.
- Terminal modification.
- Phosphorylation.
- Glycosylation.
- Fluorescent labeling.
- Biotin labeling.
Guaranteed Quality Control
- LC-MS
- HPLC-MS
Synthetic Peptide Subunit Veterinary Vaccine Development Services
Since synthetic peptide vaccines do not contain active pathogens, they have a low risk of infection, and their synthesis and preparation are relatively easy to control and highly standardized and reproducible. However, there are some challenges in the development process, such as persistence of the immune response and immune escape. BioVenic offers a range of follow-on development services, such as formulation development to increase the immunogenicity of vaccines through the addition of adjuvants, followed by a series of in vitro and in vivo assessment programs to help you successfully develop your synthetic peptide subunit vaccine.
Why Choose Us?
- One-stop service completion.
- Utilization of innovative technology platforms.
- Guaranteed full and rapid delivery.
- Batch stability assured.
The preparation process of synthetic peptide subunit veterinary vaccines avoids the use of live pathogens, reducing potential safety risks and ensuring a safer and more controlled vaccine application. BioVenic offers development services for synthetic peptide subunit veterinary vaccines, equipped with advanced technological platforms capable of efficiently synthesizing specific peptide segments related to animal pathogens. If you have the need for the development of such veterinary vaccines, please feel free to contact us. We are dedicated to providing high-quality services to meet your research and development requirements for livestock, poultry, companion animals, aquaculture species and more.
Reference
- Duro-Castano, Aroa; et al. "Peptide-based polymer therapeutics." Polymers 6.2 (2014): 515-551.