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Veterinary PCR Kit Development
Polymerase chain reaction (PCR), an extensively employed method in molecular biology, serves to amplify DNA and RNA sequences. PCR has become a key tool in animal health, covering disease genetic analysis, pathogen detection, cancer investigation, and biomarker assessment. Notably, the domain of veterinary diagnostics has witnessed an upsurge in demand for PCR assays in recent times. PCR kits have emerged as a favored resource for identifying and quantifying diverse pathogens in animals. BioVenic, as an animal disease diagnostics CRO, is committed to developing veterinary diagnostic PCR kits for animal health researchers to meet this emerging need.
PCR Kit Formats
Several different formats of PCR kits can be used for veterinary diagnostics. In addition to conventional PCR kits, real-time fluorescent quantitative PCR (qPCR) kits, quantitative reverse transcription PCR (RT-qPCR) kits, multiplex real-time PCR kits, and digital PCR kits are currently more popular.
Fig. 1 Workflow diagram of real-time PCR.1
Veterinary PCR Kit Development Services
BioVenic provides comprehensive veterinary PCR kit development services, starting from primer design synthesis to PCR kit manufacturing. With advanced equipment and technology, we ensure that each PCR kit is of the highest quality and satisfies customers. Our services mainly include the following items.
Custom Format Service
The popular format development services we provide include veterinary diagnostic PCR, qPCR, RT-PCR, RT-qPCR Kit development, etc. Whichever you choose, we provide you with high-quality development results.
Custom Formulation Service
We customize the catalog of qPCR reagent formulations to meet your specific needs, including custom mix formulations, DNA and RNA markers, formulating optimal concentrations, custom pre-amplification primers, and more.
Custom Packaging Service
Improve your laboratory's efficiency and convenience with our custom packaging services. We provide you with full-size, inner and outer packaging, custom kits, custom labels, and other service options.
Contract IVD Manufacturing
Based on your research requirements, we offer small-scale production for the lab or scale-up veterinary PCR diagnostic kit manufacturing.
Veterinary PCR Kit Development Process
Target Sequence Selection
Our team of experts helps select target sequences specific to the target of interest to avoid cross-reactivity.
Primer and Probe Design 
We offer primer and probe design services for specific target DNA or RNA sequences, taking into account factors such as primer specificity, annealing temperature, and amplicon size.
PCR Kit Component Selection 
We help customers choose the appropriate enzyme mixture, lysate, buffer, positive control, and negative control, etc., according to the type of sample and kit.
PCR Validation
Our development team helps customers validate PCR kit specificity, analytical sensitivity, and reproducibility using samples, negative and positive controls.
Optimization of PCR Reaction Conditions
We provide PCR kit optimization services, including optimizing the annealing temperature, MgCl2 concentration, primer concentration, etc., to ensure efficient amplification of the target sequence.
Why Choose Us?
Custom PCR kit design.
We can design custom veterinary PCR kits according to your specific needs, whether for research purposes or preclinical diagnostic applications.
Veterinary PCR expertise
Our expert team has extensive experience in veterinary PCR and guide and support you throughout the kit development process.
Comprehensive verification
All of our veterinary PCR kits are fully validated to ensure they perform as expected.
BioVenic is committed to providing quality veterinary PCR kit development services to our customers. We are confident in helping customers conduct animal disease diagnostic research at the genetic level. Please contact us to learn more about our PCR kit development services.
Reference
- Ishmael, Faoud T., et al. "Principles and applications of polymerase chain reaction: basic science for the practicing physician." Annals of Allergy, Asthma & Immunology 101.4 (2008): 437-443.