Veterinary Therapeutic Protein and Peptide Delivery System Development

Protein and peptide-based drugs hold significant potential as therapeutic agents for animal use due to their higher efficacy and reduced toxicity compared to conventional chemical drugs. However, challenges in their delivery have constrained broader applications. Specifically, these biotherapeutics often exhibit low oral bioavailability and encounter absorption challenges when delivered transdermally. To overcome these obstacles, BioVenic has developed an advanced delivery system for veterinary therapeutic proteins and peptides aimed at improving their properties during preclinical research. Our system incorporates various strategies, such as nanoparticle carriers, engineered exosomes, liposome encapsulation, and chemical modification of protein and peptide structures, to improve delivery efficiency and therapeutic outcomes.

Protein and Peptide Delivery System Development Overview

Proteins and peptides are essential in veterinary medicine due to their diverse functions and critical roles in managing animal diseases. However, their physicochemical properties pose challenges for oral administration, as they are unstable in the gastrointestinal tract and exhibit low bioavailability and short half-lives. Over the past few decades, this has been a focal point of extensive research. At BioVenic, a head in veterinary therapeutic applications, we have pioneered innovative strategies to improve these properties. Our efforts support our clients in enhancing delivery systems and increasing drug efficacy throughout the preclinical research phase.

Engineered Exosome Service

BioVenic is dedicated to providing a powerful tool for efficient, targeted protein delivery using an engineered exosome platform. Exosomes are small vesicles secreted by various cell types, including stem cells, immune cells, and cancer cells. They play a crucial role in intercellular communication by transferring bioactive molecules, such as proteins, nucleic acids, and lipids, between cells. Engineered exosomes are modified exosomes that have been specifically designed to enhance their protein delivery capabilities.

Fig.1 Techniques for delivering various cargos to targeted tissues using engineered exosomes. ^1,2

• Benefits of Engineered Exosomes for Protein Delivery
  • Enhanced Targeting: By engineering exosomes with specific proteins, we can precisely target desired cell types or tissues. This targeted approach ensures efficient protein delivery to the desired site, minimizing off-target effects.
  • Stability and Protection: Engineered exosomes provide a protective environment for the delivered proteins, shielding them from degradation and enzymatic activity. This enhances the stability and bioavailability of the cargo proteins.
  • Cellular Uptake and Intracellular Delivery: Engineered exosomes have natural mechanisms for cellular uptake, allowing efficient internalization of cargo proteins. They can deliver proteins directly into the cytoplasm or specific subcellular compartments, ensuring effective delivery and functional expression.
  • Biocompatibility and Safety: Exosomes are derived from endogenous cells and are generally well-tolerated by the body. They exhibit low immunogenicity and have a reduced risk of adverse immune responses compared to other delivery systems.

Nanoparticle Development Service

In the realm of veterinary application, the delivery of peptides and proteins poses a unique challenge due to their fragile nature and susceptibility to degradation. To overcome these obstacles, BioVenic, a leading biologics company, specializes in the development of nanoparticles for the delivery of peptides and proteins. With our cutting-edge technology and expertise in nanoparticle development, we offer comprehensive services to revolutionize drug delivery during protein and peptide preclinical research. As an advanced drug delivery carrier, nanoparticles can encapsulate biological agents and change the pharmacokinetic properties of peptides and proteins.

Fig.2 Discovery of artificial bioactive peptides and their conjugation with nanoparticles for biomedical applications. ^3,4

• Benefits of Nanoparticle Development for Protein and Peptide Delivery
  • Enhanced Stability and Targeting: Using nanoparticles as carriers, peptides, and proteins can be protected from damage by the internal environment while improving the bioavailability and targeting effect of drugs.
  • Controlled Release: Nanoparticles can be engineered to release their cargo at a controlled rate, ensuring a sustained therapeutic effect that can reduce the frequency of dosing and improve patient compliance.
  • Reduced Immunogenicity: Nanoparticle encapsulation can shield proteins and peptides from premature immune recognition, minimizing the risk of immune reactions and enhancing the therapeutic efficacy of the drugs.
  • Increased Solubility: Nanoparticles can improve the solubility of hydrophobic peptides and proteins, facilitating their transport and absorption within the body, which is crucial for effective drug delivery.
  • Enhanced Penetration: Nanoparticles can be designed to penetrate tough biological barriers, such as the blood-brain barrier, which enables the delivery of therapeutic agents to otherwise inaccessible sites within the body.

Liposome Encapsulation Service

Liposome encapsulation is an innovative technique for delivering proteins and peptides, involving their enclosure within liposomes—spherical vesicles made of phospholipid bilayers. This method enhances the stability, bioavailability, and delivery efficiency of therapeutic agents by protecting them from the external environment. It is particularly beneficial for drugs requiring targeted delivery and controlled release. At BioVenic, we specialize in this versatile technology, offering customized solutions to improve therapeutic outcomes in preclinical and clinical settings.

Fig.3 Techniques involved in the surface alteration of liposomes and their types. ^5,6

• Benefits of Liposome Encapsulation for Protein and Peptide Deliver
  • Sustained Release and Stability: Liposomes provide sustained release of peptides and proteins while protecting them from degradation during drug delivery.
  • Flexible and Tunable Formulations: The flexible architecture and composition of liposomes allow for highly tunable formulations to suit specific therapeutic needs.
  • Enhanced Tissue Permeability: Liposomes increase the tissue permeability of protein and peptide formulations, improving their efficacy and absorption in targeted areas.
  • Diverse Delivery Options: Liposome technology expands the delivery modes for therapeutic proteins and peptides, enabling various administration routes including parenteral, oral, pulmonary, nasal, ocular, and transdermal.

Service Workflow

BioVenic specializes in the development and delivery of veterinary therapeutic proteins and peptides. The service workflow begins with an initial consultation, where clients discuss their specific needs and goals, ensuring a customized approach. This is followed by project design and contract signing, where a detailed project plan is formulated and agreed upon by both parties. Once the contract is signed, our team executes the service, focusing on the development and formulation of therapeutic products with rigorous in-process testing for compliance and efficacy. After production, the products undergo a final quality control assessment to ensure they meet the highest standards of safety and effectiveness. The approved products are then packaged and delivered, with comprehensive reports on the production process and product specifications. To support the client post-delivery, ongoing technical support is available, addressing any further inquiries or needs to ensure client satisfaction and the optimal use of the therapeutic products.

Fig.4 The veterinary therapeutic protein and peptide delivery service workflow. (BioVenic Original)

Why Choose Us?

At BioVenic, we specialize in elevating the standards of veterinary healthcare through innovative delivery technologies for therapeutic proteins and peptides. Our expertise ensures that your preclinical research benefits from the high quality and effective solutions available in the market. We invite you to reach out to discuss how our tailored delivery systems can meet your specific research needs and accelerate your development timelines. Contact us today to learn more about our products and how they can be integrated into your veterinary applications. Let us assist you in advancing your research with precision and efficiency.

References

1. Xu, Mengqiao, et al. "Recent advancements in the loading and modification of therapeutic exosomes." Frontiers in Bioengineering and Biotechnology 8 (2020): 586130.
2. Image retrieved from Figure 1 "Methods of loading different cargos to specific tissues through engineered exosomes". Xu, Mengqiao, et al., 2020, used under CC BY 4.0, the title was changed to " Techniques for delivering various cargos to targeted tissues using engineered exosomes".
3. Jeong, Woo-jin, et al. "Peptide–nanoparticle conjugates a next generation of diagnostic and therapeutic platforms?." Nano Convergence 5 (2018): 1-18.
4. Image retrieved from Figure 2 "Discovery of artificial bioactive peptides and their conjugation with nanoparticles for biomedical applications". Jeong, Woo-jin, et al., 2018, used under CC BY 4.0, the title was changed to " Discovery of artificial bioactive peptides and their conjugation with nanoparticles for biomedical applications".
5. Nikolova, Maria P., Enamala Manoj Kumar, and Murthy S. Chavali. "Updates on responsive drug delivery based on liposome vehicles for cancer treatment." Pharmaceutics 14.10 (2022): 2195.
6. Image retrieved from Figure 1 "Surface modification strategies of liposomes, together with their classification". Nikolova, Maria P, et al., 2022, used under CC BY 4.0, the title was changed to " Techniques involved in the surface alteration of liposomes and their types ".