Veterinary Therapeutic Protein and Peptide Structural Biology Analysis

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Veterinary Therapeutic Protein and Peptide Structural Biology Analysis

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Overview Analysis Services Structure Predictions Application Service Workflow Why Choose Us?

The primary structure of a protein is fundamental to defining its activity in the development of veterinary therapeutic proteins and peptides. However, understanding the protein's stability, folding, structure, and functional activity requires comprehensive protein and peptide structural biology analysis. To address this relationship between structure and function, structural biology makes use of some fundamental concepts related to the hierarchical assembly of proteins and peptides. BioVenic provides specialized services to support and advance your veterinary therapeutic protein and peptide development projects. We offer structural biology services through gene to structure. Our structural biologists are experienced in working flexibly with you to design a program or study from our comprehensive suite of assays and services that fully support your research goals, resources, and project criteria.

Protein and Peptide Structural Biology Analysis Overview

Structural biology is a field dedicated to the study of the structure of molecules that form living matter, including proteins, nucleic acids, lipid membranes, and carbohydrates. The precise and accurate characterization of protein structure and protein complexes is essential for understanding protein function and the mechanisms of action in a biological system. By integrating data from multiple methods, the analysis offers a comprehensive understanding of molecular architecture and stability, crucial for optimizing therapeutic efficacy and safety. Solving the structure of large dynamic complexes often requires integrating several complementary techniques, such as mass spectrometry (MS) and cryo-electron microscopy (cryo-EM)—an approach known as integrative structural biology.

Fig.1 A schematic representation of a typical SRP biosensor showcases the core components and design principles. (BioVenic Original) Fig.1 Protein structure levels: peptide chains, secondary structures, and a tertiary fold example. ^1,2

Protein and Peptide Structural Biology Analysis Services

There are many techniques used to address structural biology limitations. All these techniques possess strengths and limitations and thus, scientists usually combine some of them to achieve a more complete description of the studied system. The main techniques used in structural biology are:

Types	Description	Services
Primary Structure, Secondary Structure, Tertiary Structure and Quaternary Structure	Primary structure: it is defined by the distribution or sequence of amino acids in the polymer chain. Secondary structure: When a polypeptide sequence contains a series of consecutive amino acids displaying the same dihedral angles, the polymeric chain forms regular structures. Tertiary Structure: The overall three-dimensional structure of a single polypeptide chain, including the spatial arrangement of all atoms. Quaternary structure: The arrangement and interaction of multiple polypeptide chains in a multi-subunit complex.	Determination of amino acid sequence. Mass spectrometry analysis. Circular dichroism (CD) spectroscopy. Fourier-transform infrared (FTIR) spectroscopy. Nuclear magnetic resonance (NMR) spectroscopy
Structural Domains	Distinct functional and/or structural units within a protein that may fold independently of the rest of the protein and often confer specific functions.	Domain-specific mutagenesis analysis. Domain identification and characterization.
Structural Motifs	Short, conserved sequences within a protein that form specific structures and are often associated with particular functions.	Motif identification and mapping. Functional analysis of motifs. Comparative sequence analysis.
Protein/peptide Shape	The overall shape or conformation of a protein or peptide, which is critical for its function.	Molecular modeling and simulations.
Protein/ peptide Dynamics	The study of the movements and conformational changes within a protein or peptide over time.	Molecular dynamics simulations. Hydrogen-deuterium exchange mass spectrometry (HDX-MS). NMR relaxation studies.

In-silico Protein/peptide Structure Predictions

Proteins play a central role in every biological process, performing a wide variety of functions. These functions are closely related to their native structure. For this reason, one of the purposes of structural biology is the elucidation of protein structures to understand how they work. As explained before, solving protein structures is a challenging process that can be expensive and time-consuming. In this regard, the development of increasingly powerful informatics tools has led to the expansion of the field of In-silico protein structure prediction. This discipline is focused on the inference of the 3D organization of proteins from their amino acid sequence using computational methodologies. Since proteins display a hierarchical structural organization, In-silico approaches can be applied to predict secondary, tertiary, or quaternary structures. As organization complexity increases, structural prediction becomes more arduous, and it is more difficult to get a high degree of accuracy.

Fig.1 A schematic representation of a typical SRP biosensor showcases the core components and design principles. (BioVenic Original) Fig.2 Different methods of in-silico protein/peptide structure predictions. (BioVenic Original)

Applications of Structural Biology

Identify Drug Targets
Structural biology is crucial in veterinary drug discovery, aiming to identify new chemical compounds that target biomolecules as potential treatments for animal diseases.
Study Host-pathogen Interactions
Understanding the molecular details of host-pathogen interactions is vital for comprehending disease transmission and development in animals. Structural biology helps predict and analyze these interactions, which often involve protein-protein interactions, and characterizes the biomolecules involved.
Make a Molecular Model
Using various physicochemical techniques, structural biology provides insights into the structural features of biomolecules. By integrating experimental data, molecular models can be constructed, aiding in the design of veterinary therapies and understanding the molecular basis of diseases in animals.

Service Workflow

Our workflow for therapeutic protein and peptide structural biology analysis begins with an initial consultation to understand the client's objectives and define the project scope. Once the goals are clear, we proceed to contract signing, where we finalize the terms and agreements. Following this, we design and execute the necessary experiments tailored to the specific needs of the project. After obtaining experimental data, our team performs comprehensive data analysis to interpret the structural details. Finally, we deliver a detailed report with the findings and recommendations, ensuring thorough communication and review with the client.

Fig.2 The workflow for the veterinary therapeutic protein and peptide SRP service. (BioVenic Original) Fig.3 The veterinary therapeutic protein and peptide structural biology analysis. (BioVenic Original)

Why Choose Us?

Comprehensive Structural Characterization

Detailed analysis of protein and peptide structures, including primary to quaternary levels, for thorough characterization.

Advanced Analytical Technologies

Utilization of high-resolution mass spectrometry and NMR for precise identification and quantification of structural details.

Customized Bioinformatics Solutions

Specialized tools for predicting structural elements and PTMs, providing tailored insights for specific therapeutic needs.

High Sensitivity and Accuracy

Our advanced instrumentation ensures sensitive detection and accurate analysis, crucial for identifying subtle structural variations and modifications.

To learn more about our structural biology services, which include a comprehensive analysis of the structure and modifications of veterinary therapeutic proteins and peptides, we encourage you to contact us. BioVenic's offerings encompass high-resolution techniques and specialized bioinformatics tools to ensure precise and reliable results. Our team of experts is ready to provide tailored solutions to meet your specific project needs, please contact us for more information.

References

Baldauf, Carsten, and Mariana Rossi. "Going clean: structure and dynamics of peptides in the gas phase and paths to solvation." Journal of Physics: Condensed Matter 27.49 (2015): 493002.
Image retrieved from Figure 1 "Overview over the structure levels of proteins with the chemical structure of a peptide chain, periodic and aperiodic secondary structure elements, and an example of a tertiary protein fold". Baldauf, Carsten, and Mariana Rossi.; 2015, used under CC BY 3, and the title was changed to "Protein structure levels: peptide chains, secondary structures, and a tertiary fold example ".