Veterinary Therapeutic Protein and Peptide Surface Plasmon Resonance (SPR) Analysis

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Veterinary Therapeutic Protein and Peptide Surface Plasmon Resonance (SPR) Analysis

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Overview Surface Plasmon Resonance Service Features Service Workflow Why Choose Us

Surface plasmon resonance (SPR) biosensors have rapidly become a standard tool in the pharmaceutical and biotechnology industries. This technology has label-free, real-time properties, which can be utilized to monitor the interactions of therapeutic proteins and peptides. BioVenic offers comprehensive SPR services for analyzing the binding interactions of veterinary therapeutic proteins and peptides, serving clients globally. Our state-of-the-art SPR technology and expert team ensure precise and reliable data, aiding in the development and optimization of therapeutic candidates. Whether you are in the early stages of research or advancing through preclinical trials, our SPR services provide critical insights to drive your projects forward.

Protein and Peptide Surface Plasmon Resonance Overview

Surface plasmon resonance (SPR) is an optical phenomenon that involves the transfer of light waves (photons) into electron waves (plasmons) at a thin metal layer. Plasmon waves are sensitive to the refractive index of the medium near the surface through which they travel. SPR biosensors exploit this property to monitor molecular interactions without the need for labeling either reactant. In an SPR experiment, one binding partner (protein/peptide) is immobilized on the surface, while the other partner flows across it (target/receptor). As complexes form, the accumulation of mass on the surface alters the solvent's refractive index, which the SPR detector can monitor in real time.

Fig.1 A schematic representation of a typical SRP biosensor showcases the core components and design principles. (BioVenic Original) Fig.1 Schematic of a typical SRP biosensor. (BioVenic Original)

Protein and Peptide Surface Plasmon Resonance Service

	Description	Services
Kinetic Analysis	Kinetic analysis via SPR measures the rates of association (Kon) and dissociation (Koff) of a protein or peptide interacting with its binding partner. This provides a detailed understanding of the interaction dynamics over time.	Prepare and pre-process samples. Optimize conditions to immobilize the protein or peptide on the SPR sensor chip. Offer precise kinetic analysis, enabling the determination of on-rates and off-rates for veterinary therapeutic proteins and peptides. Capture the association and dissociation phases of the interaction.
Affinity Determination	This data assesses the strength of the interaction between a protein or peptide and its binding partner, quantified as the equilibrium dissociation constant (Kd). By analyzing the equilibrium state where the rates of association and dissociation are equal, SPR provides the Kd value, reflecting how tightly the ligand binds to the analyte.	Set up a gradient of analyte concentrations to reach equilibrium. Provide accurate affinity measurements, crucial for selecting high-affinity candidates in the development of veterinary therapeutics. Determine the equilibrium dissociation constant (Kd) to quantify binding strength.
Stoichiometry	Stoichiometry analysis determines the binding ratio between a protein or peptide and its interacting partner. SPR measures the amount of analyte bound to the immobilized ligand, allowing the calculation of the binding ratio and providing insights into the molecular interaction's complexity.	Add precise concentrations of the interacting partner to determine binding ratios. Analyze the binding data to elucidate interaction stoichiometry.
Competition	Competition assays evaluate how different molecules compete for binding to the same target site on a protein or peptide. SPR detects the presence of competing molecules by measuring changes in binding response when multiple analytes are introduced, providing insights into competitive binding dynamics.	Conduct binding assays with the primary analyte followed by the competitor. Measure how the competing analyte affects the binding of the primary analyte. Quantify competitive binding and determine IC50 or Ki values.
Cooperativity	Cooperativity analysis examines how the binding of one molecule influences the binding of another molecule to a protein or peptide. SPR measures binding interactions in the presence of multiple ligands, revealing whether binding events are independent or cooperative (positive or negative cooperativity).	Measure binding interactions in the presence of multiple ligands. Provide detailed analysis and reports to clients.
Reversibility	Reversibility analysis assesses the ability of a protein or peptide to release its binding partner after forming a complex.	Provide detailed reversibility analysis services. Optimize conditions for effective binding and release cycles.

Protein and Peptide Surface Plasmon Resonance Features

Label-free Detection: No need for fluorescent or radioactive labels, preserving the native state of the molecules.
High Sensitivity: Capable of detecting low-abundance interactions with high precision.
Versatility: Suitable for a wide range of biomolecules, including small molecules, proteins, peptides, nucleic acids, and lipids.

Protein and Peptide Surface Plasmon Resonance Service Workflow

SPR services begin with meticulous sample preparation to ensure high-quality, functional proteins and peptides. Our expert team then designs tailored experiments to address specific research objectives. During data acquisition, real-time binding interactions are monitored and recorded. This is followed by comprehensive data analysis to extract kinetic and affinity parameters. Finally, we compile detailed reports, providing clear, actionable insights to support the development of veterinary therapeutics.

Fig.2 The workflow for the veterinary therapeutic protein and peptide SRP service. (BioVenic Original) Fig.2 The veterinary therapeutic protein and peptide surface plasmon resonance service workflow. (BioVenic Original)

Why Choose Us?

We utilize cutting-edge technology to provide high-precision kinetic analysis, affinity determination, stoichiometry, competition, cooperativity, and reversibility services, ensuring comprehensive data on protein and peptide interactions.

With years of expertise in veterinary therapeutic protein and peptide development, our team understands the unique demands of the industry and offers tailored solutions to support your preclinical research and product development success.

From sample preparation, experimental design, and data acquisition to detailed reporting, we offer an end-to-end service ensuring efficiency and accuracy at every step, safeguarding the success of your project.

BioVenic offers specialized SPR services for the identification and characterization of veterinary therapeutic proteins and peptides. Our advanced technology ensures precise and comprehensive evaluations, including kinetic analysis, affinity determination, stoichiometry, competition, cooperativity, and reversibility. Trust BioVenic to provide the critical insights needed for your preclinical research and development. Contact us today to discuss your project requirements and request a quote.