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Veterinary Viral Antigen Identification by RAPTR
Identifying veterinary viral antigens is crucial for understanding animal viral diseases and analyzing viral pathogens rapidly. Immunodetection is a specific method for identifying viral antigens, and the emergence of receptor-antigen pairing by targeted retroviruses (RAPTR) technology has significantly accelerated the efficiency of identifying viral antigens through immunodetection. As a professional biotechnology company, BioVenic has established an antigen identification platform based on RAPTR, providing clients with a one-stop solution for veterinary viral antigen identification. We are committed to assisting clients in capturing antigens that specifically bind to receptors while offering a landscape of potential antigens.
Advanced RAPTR Technology Platform
Our RAPTR technology platform utilizes genetically modified lentiviruses to achieve precise pairing of receptors and antigens. By combining viral pseudotyping and molecular engineering, we introduce the antigen-coding genes of veterinary viruses into retroviruses, enabling the display of antigens on the surface of retroviruses. This modification allows the engineered retroviruses to specifically bind to different animal T or B cell receptors in an antigen-specific manner. Through single-cell sequencing, accurate detection of both antigen and receptor identities is achieved.
Fig. 1 Antigen-TCR-specific recognition analysis based on the RAPTR platform.1
Fig. 2 Antigen-BCR-specific recognition analysis based on the RAPTR platform.1
Featured Veterinary Viral Antigen RAPTR Identification Services
We offer fully custom, one-stop veterinary viral antigen identification services based on the RAPTR technology platform for our clients. All aspects of viral vector construction, library preparation, single-cell sequencing, and data analysis are covered by our services. Here are some of our featured service offerings.
Viral Vector Construction
We construct retroviral vectors according to customer needs, usually choosing lentivirus as the vector. We help customers clone and insert the target antigen gene into an expression vector, and optimize regulatory elements to ensure optimal expression in host cells. Rigorous quality control and validation are conducted to ensure high infectivity and expression of the target antigen gene.
TCR and BCR Libraries Preparation
We aid clients in extracting total RNA from T or B cells of host animals, synthesizing cDNA, and amplifying the variable regions of TCR or BCR. Subsequent sequence sequencing and analysis involve inserting diverse sequences into library vectors to construct TCR or BCR libraries. Through quality control and validation, we ensure that libraries exhibit high diversity and coverage.
Single-cell Sequencing
Infecting cells expressing host animal T or B cell receptors with engineered retroviral vectors may lead to antigen-specific recognition. We help clients in single-cell sorting of infected cells, ensuring each single cell carries the same TCR or BCR. Total RNA is extracted from each single cell, and cDNA is synthesized. The variable regions of the target TCR or BCR are then amplified via PCR.
Data Analysis
We analyze single-cell sequencing data to help clients obtain TCR or BCR diversity information for each single cell, generating interaction maps between antigens and receptors. Additionally, we offer custom data analysis services tailored to the specific requirements of client's projects to meet diverse research needs.
Workflow of Veterinary Viral Antigen Identification by RAPTR
Applications
Why Choose Us?
High Efficiency
Our RAPTR technology platform enables simultaneous screening of multiple veterinary virus antigens, enhancing screening efficiency.
Scalability
RAPTR technology is modular and can be expanded and customized according to customer's needs, catering to various research requirements.
High Throughput Screening
RAPTR technology rapidly identifies numerous antigen-receptor interaction pairs, accelerating the antigen recognition and screening process.
The RAPTR platform provides an accelerated means for the identification of veterinary viral antigens. It not only promotes the discovery of viral antigens but also provides strong technical support for in-depth research on virus transmission mechanisms, vaccine and immunotherapy development. By choosing the RAPTR-based veterinary virus antigen identification service provided by BioVenic, customers will enjoy efficient multi-antigen screening, a scalable modular platform, and rapid high-throughput antigen identification capabilities. If you have any further inquiries, please feel free to contact us at any time.
Reference
- Dobson, Connor S., et al. "Antigen identification and high-throughput interaction mapping by reprogramming viral entry." Nature Methods 19.4 (2022): 449-460.