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Veterinary Virus Targeted Sequencing
The outbreak of infectious diseases in animals has profound implications for both animals. Apart from posing a significant threat to health, it also results in substantial economic losses. However, laboratory testing for the pathogens of animal infectious diseases, especially the mixed infections of various viruses, presents challenges for animal health researchers. Traditional virus cultivation and PCR testing are time-consuming and not conducive to early diagnosis of animal infectious diseases. On the other hand, NGS can simultaneously detect millions of DNA templates. BioVenic is dedicated to providing veterinary virus targeted sequencing services, helping clients to concurrently test multiple samples and or detect various target sequences efficiently.
Veterinary Virus Targeted Sequencing Services
We offer targeted sequencing services that cater to any virus gene sequence of interest to the client, featuring high specificity and customizability. We help clients with targeted analysis of specific characteristics of veterinary viruses, such as virulence, resistance, mutations, and more. Our comprehensive virus targeted sequencing solution includes the following featured services.
Custom Design of Amplicon Panel Primers
The design of the sequencing panel is a crucial aspect of our service. We tailor panel primers based on specific regions of interest in the target virus or virus genome chosen by the client. We select conserved sequences from known virus reference genomes to design panel primers with strong specificity and high coverage, fully meeting the client's needs to simultaneously detect hundreds of sequences.
Nucleic Acid Extraction
We accept various types of samples from different animals, including livestock, companion animals, poultry, etc. These samples may include purified virus DNA, animal tissue slices, tissues, blood, swabs, etc. The challenge in targeted sequencing lies in ensuring an adequate amount of viral nucleic acid in the samples. We address this challenge by pre-sequentially enriching viral nucleic acid through methods such as high-speed centrifugation, sample filtration, or virus cultivation. Simultaneously, we use DNA enzyme treatment to remove host gDNA or employ rRNA removal to reduce host nucleic acid background in the samples.
Enrichment and Library Preparation
Through primer amplification, we specifically enrich viral target sequences for the client. For sequencing of a small number of target regions, we offer sequencing services combining PCR amplification with Sanger sequencing. For sequencing a large number of genes or target regions, we provide NGS-based sequencing services. Depending on the client's research goals and sequencing platform (Illumina NovaSeq, HiSeq, MiSeq, etc.), we prepare libraries suitable for the client.
Veterinary Virus Targeted Sequencing for Common Veterinary Viruses
Our targeted sequencing capability encompasses common veterinary DNA viruses, RNA viruses, prevalent drug-resistant genes, and viral virulence genes. It specifically targets common viral pathogens in various infection scenarios, such as gastrointestinal infections, central nervous system infections, respiratory infections, joint infections, reproductive system infections, etc. The table below illustrates the common viruses in dogs, chickens, cats, horses, and other animals that we routinely target for our clients.
| Animal Types | Common Viruses Targeted Primers |
|---|---|
| Canine | Canine parainfluenza virus (CPIV), Canine influenza virus (CIV), Canine parvovirus type 2 (CPV2), Rabies virus, Hendra virus (HeV), Canine distemper virus (CDV), etc. |
| Feline | Feline herpesvirus (FHV-1), Feline calicivirus, Nipah virus, etc. |
| Avian | Avian influenza A virus, Gallid herpesvirus 1 (GaHV-1), Infectious bronchitis virus (IBV), Newcastle disease virus (NDV), Avian encephalomyelitis virus, etc. |
| Bovine | Parainfluenza-3 virus (PI-3), Bovine enterovirus, Jena virus, West Nile virus (WNV), Akabane virus, etc. |
| Equine | African horse sickness virus (AHSV), Equine adenovirus type 1 (EAdV1), Ferret respiratory enteric coronavirus (FRECV), Equine herpesvirus-1 (EHV-1), Eastern equine encephalitis virus (EEEV), Western equine encephalitis virus (WEEV), Venezuelan equine encephalitis virus (VEEV), etc. |
| Sheep | Nervous necrosis virus (NNV), Borna disease virus, etc. |
| Porcine | Swine influenza A virus, Porcine enteric coronaviruses, Porcine adenovirus, Japanese encephalitis virus, Pseudorabies virus (PRV), Menangle virus (MenPV), Teschovirus, Porcine reproductive and respiratory syndrome virus (PRRSV), Porcine circovirus type 2 (PCV2), etc. |
Veterinary Virus Targeted Sequencing Workflow
Applications
Why Choose Us?
Detection of Genetic Variations
Our targeted sequencing helps customers discover point mutations, insertions or deletions (INDELS), copy number changes (CNVs), and gene rearrangements.
High Sensitivity for Low-abundance Viruses
Our advantage lies in its ability to detect low-abundance viruses with high sensitivity while reducing the amount of sequencing data and analysis workload and improving detection efficiency.
Simultaneous Detection of Multiple Viral Pathogens
We help customers detect multiple samples and multiple veterinary viral pathogens at the same time, reducing the difficulty of early diagnosis of mixed infections.
Targeted sequencing is a process of nucleic acid enrichment and sequencing for known viral genes. It holds significant importance in the detection and analysis of animal viral pathogens. As experts in the field of animal health, BioVenic has been providing customized veterinary virus-targeted sequencing services for an extended period. Our commitment lies in accelerating clients' research in virology, biology, and related fields by efficiently detecting specific sequences. If you are interested in our services, please feel free to contact us.
References
- Anis, Eman., et al. "Evaluation of targeted next-generation sequencing for detection of equine pathogens in clinical samples." Journal of veterinary diagnostic investigation: official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc vol. 33,2 (2021): 227-234.
- Yang, Yu., et al. "Targeted Sequencing of Respiratory Viruses in Clinical Specimens for Pathogen Identification and Genome-Wide Analysis." Methods in molecular biology (Clifton, N.J.) vol. 1838 (2018): 125-140.