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Hamster Putative Phospholipase B-like 2 (PLBD2) ELISA Kit-Sandwich

Cat. No.EK3F23

Product TypeAnimal Immunoassay Kits

Size

Product Overview

BioVenic Hamster Putative Phospholipase B-like 2 (PLBD2) ELISA Kit-Sandwich is designed for the quantitative determination of Hamster PLBD2 in serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich
Specificity The assay kit is specific for Hamster Putative Phospholipase B-like 2 (PLBD2).
Target Species Hamster
Species Reactivity Hamster
Detection Range 12.6-800 pg/mL
Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 12%
Assay Time Around 90 min
Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell lysate, and other biological fluids.

Target Information

Hamster Putative Phospholipase B-like 2 is a host-cell protein (HCP) impurity identified in therapeutic monoclonal antibodies produced using Chinese Hamster Ovary (CHO) cells. Putative Phospholipase B-like 2 (PLBD2) is predicted to possess phospholipase activity and be involved in the phospholipid catabolic process. It is found in extracellular exosomes. PLBD2 has been proposed as a residual host cell protein responsible for degrading PS20 in a drug formulation. Initial studies proposed PLBD2 as a polysorbate-degrading enzyme in drug formulations. However, subsequent genetic knock-out and immuno-depletion experiments showed no reduction in polysorbate degradation, contradicting this hypothesis.

Target/Biomarker Hamster PLBD2
Target Synonym Putative Phospholipase B-like 2; PLBD2; phospholipase B domain containing 2; PLBL2

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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