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Animal Cell Immortalization Solution
With years of theoretical and practical experience in animal physiology and cell biology, BioVenic has mastered cutting-edge technological methods for immortalizing animal cells. BioVenic has developed a comprehensive and efficient animal cell immortalization solution to cater to a wide range of scientific research needs of customers.
Background
The growth and metabolism of animal cells involve a dynamic balance of proliferation, differentiation, aging, and apoptosis, culminating in the end of life through continuous generation. The proliferation of normal mammalian cells in vitro is limited, and they will age and die due to programmed cell death or apoptosis. This is also one of the limitations of current in vitro research on animal cells. Cell immortalization technology involves genetic modifications of animal cells or external factors and human manipulation during in vitro culture. This process enables the cells to bypass the aging and death processes of normal cells, allowing for long-term subculture and unlimited division and proliferation.
Table 1 Overview of Animal Cell Immortalization Technologies
| Gene | Function | Applicable Scope |
| hTERT | Overexpression of the hTERT gene in animal cells not only prevents telomere shortening but also activates the telomerase activation mechanism, extending cell lifespan. | Most Cell Types |
| SV40T | SV40T antigen can bind to and inhibit the expression of several cellular tumor suppressor genes, including pRB and p53. It can also prevent cells from transitioning into the G1/S phase and G2/M phase of the cell cycle, leading cells to enter a state of unlimited proliferation. | Most Cell Types |
| EBV | EBV lurks in lymphocytes for a long time and expresses a variety of latent genes. It interacts with oncogenes, causing cell cycle disorders and inhibiting apoptosis, thereby promoting unlimited cell proliferation. |
B Lymphocytes Oropharyngeal Epithelial Cells |
| HPV-E6/E7 | The combined action of HPV E6 and E7 proteins stimulates cells to keep proliferating and dividing. The immortalizing activity of the E6 protein is associated with binding and inactivating the p53 protein, whereas the immortalizing activity of the E7 protein is linked to binding and dissociating the E2F-Rb complex. The E6 protein can also activate the TERT gene, enhancing the expression and activity of telomerase. | Epithelial Cells |
| CDK4 | CDK4 plays a crucial role in cell cycle regulation and tumorigenesis. It is a type of proto-oncogene that can promote the process of cell immortalization. | Epithelial Cells |
| cMyc | cMyc can inhibit replicative senescence caused by telomere damage. It is also a gene that can enable cells to proliferate indefinitely, gain immortalization function, and promote cell division. | Epithelial Cells |
Fig.1 Animal Primary Cell Immortalization.
Animal Cell Immortalization Services
Considering the diverse research goals and needs of scientific researchers, BioVenic categorizes the technical services for customized and stable immortalized cell lines into several categories based on the mechanism of immortalization of animal cells. Our service areas mainly include immortalization caused by the expression of telomerase reverse transcriptase, virus-induced immortalization, and immortalization caused by proto-oncogene activation/ tumor suppressor gene inactivation.
hTERT Exogenous Stable Expression
BioVenic offers advanced technical services to achieve immortalization in animal cells by stably expressing human telomerase reverse transcriptase (hTERT). This process helps maintain adequate telomere length to prevent cell senescence caused by replication. Our laboratory technical team has extensive project experience in implementing hTERT-mediated cell immortalization on economically significant animal cells. During the process, we continuously optimize parameters to achieve stable expression, ensuring the high quality and efficiency of the solution.
Viral Gene-induced Immortalization
BioVenic has introduced various methods for virus-mediated immortalization of animal cells. Our expert team will match and design the most feasible and efficient technical solutions for your specific scientific research goals and cell types. EBV, HPV E6/7 genes, and SV40 T antigen have been harnessed and utilized to induce the immortalization of animal cells. These viral genes achieve immortality by inactivating a series of tumor suppressor genes, which induce target cells to enter a state of replicative senescence.
Proto-oncogene Activation and Tumor Suppressor Gene Inactivation
BioVenic supports the immortalization of animal cells by activating proto-oncogenes and inactivating or knocking out tumor suppressor genes. Our technicians utilize state-of-the-art mammalian genetic modification and editing techniques to manipulate the expression regulatory elements of these two genes. This manipulation supports cells to surpass their normal life limits and reach a state of unlimited proliferation post-cancer, ultimately leading to immortality. Our laboratory team continues to optimize the technical processes, and is dedicated to offering customers efficient and stable solutions.
Animal Cell Immortalization Applications
- Immortalized animal cells are ideal models for scientific research on cell proliferation, differentiation, and apoptosis in vitro.
- Cell immortalization research is an important technology that supports the in vivo and external exploration of animal tumorigenesis mechanisms.
- Immortalization breaks through cell generation barriers and provides researchers with more abundant and convenient cell resources.
Why Choose Us?
BioVenic offers a variety of immortalization solutions for animal cells to cater to a broad spectrum of scientific research requirements and objectives.
BioVenic has developed a sophisticated mammalian cell virus transfection technology platform to ensure the effective implementation of cell immortalization.
BioVenic has a team of experienced experts in the field of animal cell engineering technology to provide professional and timely technical support.
BioVenic has a top-tier cell laboratory team with extensive experience in animal cell projects. They utilize cutting-edge cell immortalization technology and offer customized services tailored to meet the unique requirements of each project. Our immortalization solutions combine multiple technologies, such as stable expression of animal cells, viral transfection, and gene editing, to overcome challenges in the in vitro culture of animal cells. If you are working on an animal cell immortalization project, please contact us and let us be your right-hand support.