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Animal Feed Probiotics Development

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Feed Probiotics Solutions Strain Development Safety and Efficacy Assessment Fermentation Solution Animal Feed Probiotics Development Workflow Why Choose Us?

Probiotics consist of carefully selected live strains of microorganisms that, when administered in appropriate quantities, provide health benefits to the host. When used in animal feed, probiotics offer various advantageous effects, such as enhancing growth performance, improving gastrointestinal health, boosting immunity, and enhancing product quality. BioVenic offers a wide range of specially designed solutions for the development of probiotics for animal feed. Our solutions encompass several probiotic genera and incorporate advanced integrated technologies.

Animal Feed Probiotics Solutions

For commonly used probiotic types, we provide customized services to help researchers explore their characteristics and the effects of their application as animal feed additives.

Bacillus

Our solutions focus on the development of Bacillus strains as probiotics for animal feed. Bacillus species, including Bacillus subtilis and Bacillus cereus, have the ability to form spores and produce enzymes, antibiotics, and other bioactive substances. These properties enhance animal performance and health, mitigate disease effects, and improve product quality.

Enterococcus

We offer state-of-the-art solutions for the development of Enterococcus strains as animal feed additives. Enterococcus plays a crucial role in regulating the immune systems of both humans and animals. Through biochemical, enzymatic, and molecular assays, our solutions assist animal nutrition researchers in exploring the potential benefits of Enterococcus as probiotics in animal feeds.

Lactobacillus

Our professional team provides comprehensive services for developing Lactobacillus probiotics, including isolation and identification of Lactobacillus strains, genetic research, safety and efficacy evaluations, and fermentation research. These services aim to expand the application of Lactobacillus feed additives in animals.

Bifidobacterium

Our Bifidobacterium feed probiotic development solutions are specifically designed to assist animal nutrition researchers in developing Bifidobacterium strains that are well-suited for specific gastrointestinal environments in animals. We ensure guaranteed safety and efficacy through stringent quality control measures to maintain strain stability and viability in the final product.

Pediococcus

With our development solutions, we aid in isolating, identifying, and evaluating novel Pediococcus strains. Pediococcus acidilactici and Pediococcus pentosaceus are commonly utilized Pediococcus species with probiotic functions in animals.

Streptococcus

The Streptococcus genus consists of both commensal and pathogenic species. To develop Streptococcus strains as animal feed probiotics, our services include multiple safety evaluation methods, as well as in vivo and in vitro efficacy assessments.

Yeasts

A diverse range of yeasts can be used as animal feed probiotics. Our comprehensive yeast probiotic development solution offers a one-stop approach for researchers to explore the properties and effects of different yeast probiotics, enabling better applications in animal nutrition.

Animal Feed Probiotics Strain Development

Developing probiotic strains for animal feed involves a comprehensive study encompassing safety, functionality, and technical usability of the strains. Please refer to Table. 1 for detailed information on these aspects.

Table. 1 Selection criteria and required properties of probiotic strains1

Safety
  • Animal origin.
  • Isolated from the gastrointestinal tract of healthy individuals.
  • History of safe use.
  • Precise diagnostic identification (phenotype and genotype traits).
  • Absence of data regarding an association with infective disease.
  • Absence of the ability to cleave bile acid salts.
  • No adverse effects.
  • Absence of genes responsible for antibiotic resistance localized in non-stable elements.
Functionality
  • Competitiveness in respect to the microbiota inhabiting the intestinal ecosystem.
  • Ability to survive and maintain the metabolic activity, and to grow in the target site.
  • Resistance to bile salts and enzymes.
  • Resistance to low pH in the stomach.
  • Competitiveness in respect to microbial species inhabiting the intestinal ecosystem (including closely related species).
  • Antagonistic activity towards pathogens (e.g. H.pylori, Salmonella sp., Listeria monocytogenes, Clostridium difficile).
  • Resistance to bacteriocins and acids produced by the endogenic intestinal microbiota.
  • Adherence and ability to colonize some particular sites within the host organism, and an appropriate survival rate in the gastrointestinal system.
Technological Usability
  • Easy production of high biomass amounts and high productivity of cultures.
  • Viability and stability of the desired properties of probiotic bacteria during a fixing process (freezing, freeze-drying),
  • preparation and distribution of probiotic products.
  • High storage survival rate in finished products (in aerobic and micro-aerophilic conditions).
  • Guarantee of desired sensory properties of finished products (in case of food industry).
  • Genetic stability.
  • Resistance to bacteriophages.

Animal Feed Probiotics Isolation and Identification

Our services encompass the development of culture media and employ techniques such as morphological, biochemical, and genetic identification to isolate and identify probiotics from animal samples, fermented foods, and various other sources. This process provides researchers with initial materials for further investigations.

Genetic Study on Animal Feed Probiotics

We offer comprehensive capabilities, including whole genome sequencing, amplicon sequencing for bacteria and fungi, and genetic modification. These techniques enable researchers to gain insights into the beneficial properties and safety of probiotics at the molecular level. Additionally, the data obtained facilitates comparative, functional, and evolutionary studies, significantly contributing to the development of probiotics with outstanding traits for animal feeds.

Safety and Efficacy Assessment of Animal Feed Probiotics

  • Safety Assessment of Animal Feed Probiotics

Our services allow for the thorough evaluation of potential animal feed probiotics in terms of safety, both at the genetic and phenotypic levels. We assess indicators such as the presence of virulence factors, antibiotic resistance, toxin-producing genes, and the ability to produce enzymes that may be harmful to animals, such as hemolysin. By analyzing these indicators collectively, we assist in determining the suitability of a probiotic strain as a feed additive.

  • In Vitro Efficacy Assessment of Animal Feed Probiotics

Our solutions allow for in vitro studies to evaluate the efficacy of potential feed probiotics in animals. Key indicators include tolerance to the animal's gastrointestinal environment (such as low pH, digestive enzymes, bile salts, etc.), cell adhesion, bacteriostatic properties, antioxidant capacity, and enzyme production. Additionally, we offer customized efficacy studies to assess the effectiveness of animal feed probiotics.

  • In Vivo Probiotic Effect Study of Animal Feed Probiotics

To validate the potential applications of probiotics as animal feed additives, we provide a diverse range of animal in vivo models. Utilizing our platforms for feed analysis, animal metabolite analysis, and omics analysis, we not only assist researchers in exploring appropriate dosage and efficacy of probiotics but also contribute valuable data for studying probiotic mechanisms.

Probiotic Effects in Animals
Improve Gut Health Modulate Immune system Anti-inflammation
Reduce Metabolic Disorders Facilitate Digestion and Absorption Anti-oxidation

Animal Feed Probiotics Fermentation Solution

Fermentation serves as the foundation for large-scale production of probiotics for animal feed. We offer tailored solutions for different probiotic species, including the optimization of fermentation substrates, fermentation conditions (such as temperature and pH), analysis of fermentation products, and quality control of strains throughout the fermentation process.

Animal Feed Probiotics Development

Our solutions provide various forms of finished probiotic products for animal feed, including freeze-dried, liquid, and mixed forms. We offer differentiated finished products based on your specific application requirements. The quality of animal feed probiotics directly impacts their effectiveness. We implement comprehensive quality control measures throughout the development of feed probiotic strains, safety and efficacy evaluations, and fermentation processes. Key factors in quality control include strain purity and viability, as well as the stability of the product's composition and functionality.

Animal Feed Probiotics Development Workflow

Fig. 4 Animal feed probiotics development workflow (BioVenic Original)

Why Choose Us?

Advanced Technology Platform

Our advanced technology platform enables the analysis of feed, feed additives, animal metabolites, probiotic fermentation products, and more.

Expert Technical Team

Our skilled team possesses extensive research experience in animal nutrition and the development of feed probiotics. We are equipped to develop suitable products according to your specific application needs.

Efficient Service Team

Our dedicated service team excels in promptly resolving research-related challenges, driving research progress, and providing efficient solutions.

With our deep understanding of feed probiotics and access to internal and external research resources, we develop a wide range of animal feed probiotics. BioVenic's comprehensive solutions encompass strain development, safety and efficacy studies, fermentation process optimization, and finished product development. We prioritize comprehensive quality control throughout every step to deliver widely applicable and highly effective animal probiotic products. If you are interested in our probiotic solutions for animal feed, please contact us and share your research plans.

References

  1. Markowiak, Paulina, and Katarzyna Śliżewska. "The role of probiotics, prebiotics and synbiotics in animal nutrition." Gut pathogens 10 (2018): 1-20.
  2. Under Open Access license CC BY 4.0, without modification.
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