Feed Probiotics-Bacillus

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Feed Probiotics-Bacillus

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Strain Development Safety and Efficacy Fermentation Solution Probiotics Development Probiotic Effects Workflow Why Choose Us?

Bacillus is a type of Gram-positive, rod-shaped bacterium that can form spores. It can thrive in both aerobic and facultative anaerobic conditions and is widely present in nature, including in soil, air, fermented foods, and the gut of animals. The spore form of Bacillus probiotics enables their survival in extreme environmental conditions, allowing them to persist even in circumstances that would typically kill other forms of bacteria. BioVenic offers a comprehensive solution for the development of Bacillus as feed additives for livestock and companion animals. Using our advanced technical platforms, we provide a range of services including probiotic Bacillus strain development, probiotic Bacillus safety and efficacy assessment, probiotic Bacillus fermentation solution, quality control and product development. Our team of probiotics experts is highly skilled and can assist you in the development of Bacillus probiotics with exceptional feasibility and broad applicability.

Fig. 1 Microscopic image of Bacillus subtilis¹

Probiotic Bacillus Strain Development

Bacillus Isolation and Identification

BioVenic offers various methods to isolate and identify potential Bacillus probiotics from the gastrointestinal tracts and feces of different animal species such as chickens, pigs, ruminants, and aquatic animals. Our methods include 16S rRNA gene sequencing, analysis of gyrA or gyrB genes, biochemical tests, and multilocus sequence analysis.

Bacillus Genetic Background Research

Using PCR, gene sequencing, and genomic analysis techniques, we help you understand the genetic basis of Bacillus that may contribute to its potential probiotic effects. Whole-genome information analysis allows us to predict its probiotic potential by assessing the ability to produce antimicrobial peptides and extracellular effector molecules.

Bacillus Genetic Modification

Through our genetic engineering platform and integrated probiotic development platform, we help researchers obtain genetically stable Bacillus probiotics with desired traits and provide solutions to evaluate their potential as feed probiotics. In addition, our B. subtilis expression system can serve as a cell factory for the microbial production of chemicals, enzymes and antimicrobial materials for use as feed additives.

Probiotic Bacillus Safety and Efficacy Assessment

Safety Assessment of Bacillus

Ensuring the safety of Bacillus strains as animal probiotics is essential. BioVenic provides solutions to assess the toxin production ability and antibiotic resistance of Bacillus. We offer full genome sequencing and analysis to detect genes responsible for enterotoxin production and the emetic toxin. Through ELISA and cell culture assays, we determine toxin production and cytotoxicity. Additionally, we examine phenotypic antimicrobial resistance and antimicrobial resistance genes to assess whether the strain exhibits intrinsic or acquired resistance to antibiotics such as vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline, and chloramphenicol.

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In Vitro Efficacy Assessment of Bacillus as Feed Probiotics

We offer a variety of in vitro testing solutions for evaluating the suitability of Bacillus strains as probiotics for animals.

Our in vitro research solutions evaluate the tolerance of Bacillus spores and vegetative bodies to acid, bile salt, intestinal, and gastric fluids, providing information on their ability to survive, colonize, and persist in the gastrointestinal environment.

We provide solutions to measure the antimicrobial activity of Bacillus against Gram-positive bacterial pathogens, Gram-negative bacterial pathogens, and fungal pathogens.

We analyze enzymatic activities and characteristics of extracellular enzymes produced by Bacillus, such as amylase, protease, lipase, phytase, cellulase, and xylanase. We also offer solutions for developing enzyme additives from Bacillus.

In Vivo Probiotic Effect Study

To account for individual factors and determine the effects of probiotic supplementation, we conduct in vivo animal experiments using different species. We evaluate the safety and efficacy of Bacillus probiotics, considering growth performance, nutrient digestion and absorption capacity, intestinal health, and immune stimulation ability. These experiments help uncover the appropriate dosage, interactions with feed or other supplements, and the probiotic mechanisms of Bacillus specific to different animals.

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Animal Nutrition and Metabolism Solution

Animal Nutrition and Metabolism Analysis

Probiotic Bacillus Fermentation Solution

By carefully selecting the appropriate culture medium, optimizing the fermentation process, refining strains, and implementing stringent quality control measures, we offer viable solutions to support animal nutrition researchers in enhancing the probiotic Bacillus fermentation process. Our expertise spans small to large-scale production, helping increase probiotic yield and ensuring strain purity. In addition to Bacillus probiotics, our Bacillus fermentation techniques can yield enzymes, antibiotics, and other bioactive products.

Development of culture medium

We assist in adjusting the proportion of raw materials within the culture medium, taking into account their economic and nutritional value. This optimization promotes strain growth and vitality.

Optimization of fermentation process

We analyze the growth patterns of strains and the production of desired products to fine-tune critical factors such as pH levels, temperature, inoculation volume, and fermentation time. These optimizations maximize efficiency.

Quality control of
products

Leveraging our advanced detection technology platform, we meticulously screen fermentation products for harmful microorganisms and toxins. This comprehensive quality control process ensures the purity and safety of Bacillus probiotics, as well as any by-products generated in the fermentation process.

Fig. 2 Application of B. subtilis^2,3

Bacillus Probiotics Development

Our commitment to quality is ingrained in every stage of Bacillus probiotic development, fermentation and product manufacturing. We offer a comprehensive range of testing services to ensure the purity, activity, genetic and phenotypic consistency of Bacillus probiotic strains, as well as to identify any contaminants present in fermentation products. In addition, we can tailor our quality control protocols to your specific strain and requirements. Equipped with advanced product processing technology, we are able to meet the diverse needs of our customers by providing Bacillus development service in a variety of forms. Whether you require liquid, solid, lyophilized or other forms, we can accommodate your preferences and enable seamless integration of our products into various aspects of animal nutrition research.

Probiotic Effects of Bacillus on Animals

The table below highlights several Bacillus species that have undergone testing for their probiotic effects in animals. The beneficial effects of Bacillus as animal feed additives primarily involve enhancing performance and health, mitigating the effects of diseases, and enhancing product quality.

Table. 1 Effects of Bacillus probiotics on farm and companion animals

Strains	Characteristics	Effects on Animals
Bacillus subtilis	At least five strains are considered GRAS. Can grow efficiently in low-cost carbon and nitrogen sources. Have antimicrobial, antiviral, and anticancer effects.	Enhance disease resistance and promote the growth performance of pigs and chickens under disease challenge conditions. Improve gut health and fecal properties in dogs. Assist with cat Ringworm.
Bacillus cereus	Toxin-producing. Can cause intestinal illnesses.	Has the potential to improve the production of chickens, pigs, and cattle.
Bacillus coagulans	Produces lactic acid. Has therapeutic effects on intestinal diseases.	Improve growth performance, digestive enzyme activity, and meat quality in poultry. Decrease the diarrhea rate and improve the growth performance of piglets. Improve the final weight, daily weight gain, and relative weight gain of shrimp and grass carp.
Bacillus clausii	Has tolerance to heat, acid, and salt. Some strains produce and secrete riboflavin.	Improve the liver function and humoral immunity of broiler chicks.
Bacillus licheniformis	Can be found in raw milk as a contaminant. Ubiquitous in soil and food on farms.	Has the potential to overcome the challenge of bacterial LPS in animals. Relieve diarrhea, enhance antioxidant capacity, immunity function, and fecal microflora structure in weaned pigs. Function as a growth promoter and meat quality enhancer in broiler poultry.

Feed Probiotic Bacillus Development Workflow

Why Choose Us?

Our solutions are meticulously crafted for the development of animal probiotics, offering both in vitro and in vivo experimental validation to ensure that the probiotics developed hold practical application value for animals.

With a range of advanced testing technologies and product development services at our disposal, we cater to your needs for testing metabolites, proteins, genes, and more from animals and bacteria throughout the Bacillus probiotics development process.

We have extensively researched the development process of utilizing Bacillus as a probiotic and can devise a tailored research solution aligned with your specific experimental requirements.

As a dedicated solution provider in the field of Bacillus feed probiotic development, we prioritize understanding our customers' needs and provide process-based probiotic services with holistic solutions. BioVenic's services include probiotic Bacillus strain development, probiotic Bacillus safety and efficacy assessment, probiotic Bacillus fermentation solution, quality control and product development. If you're conducting research to develop Bacillus as feed probiotics, please contact us with your project details. Your information will be kept confidential and we will respond promptly to assist you.

References

From Wikipedia. By Y tambe, CC BY-SA 3.0. Without modification. https://en.wikipedia.org/wiki/File:Bacillus_subtilis_Gram.jpg.
Image retrieved from Figure 1 "Application of B. subtilis for genetic engineering, production of industrial chemicals or enzymes, agriculture, medicine and biomaterials." Su et al., 2020, used under [CC BY 4.0]. The original title was changed to "Application of B. subtilis".
Su, Yuan, et al. "Bacillus subtilis: a universal cell factory for industry, agriculture, biomaterials and medicine." Microbial cell factories 19 (2020): 1-12.