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Feed Probiotics-Pediococcus

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Strain Development Safety and Efficacy Fermentation Solution Probiotics Development Probiotic Effects Workflow Why Choose Us?

Pediococcus microorganisms are characterized by their spherical shape, Gram-positive nature, non-motility, absence of catalase activity, and ability to thrive in aerobic to microaerophilic environments. BioVenic provides a comprehensive range of solutions for the development of Pediococcus probiotics as additives for animal feed. Our integrated platform offers services for strain development, safety and efficacy assessment, as well as fermentation solutions tailored to facilitate the development of Pediococcus probiotics for feed applications. With stringent quality control measures implemented throughout the entire solution process, our dedication is to deliver satisfactory solutions for the development of finished Pediococcus probiotics products.

Fig. 1 Scanning electron micrograph of Pediococcus pentosaceus G6 (Damayanti, et al. 2021)Fig. 1 Scanning electron micrograph of Pediococcus pentosaceus G61

Strain Development for Probiotic Pediococcus

  • Pediococcus Isolation and Identification

Utilizing various morphological, physiological, and biochemical tests, our solutions enable the isolation of lactic acid bacteria (LAB) from fermented foods and other samples. Through 16S rRNA gene sequencing and analysis, we provide a molecular identification tool for potential Pediococcus probiotics.

  • Genetic Study on Pediococcus

Our genetic study platform employs advanced techniques such as whole-genome sequencing, 16S rRNA sequencing, and other genetic tools to support various research efforts on Pediococcus bacteria. This includes molecular identification, phylogenetic studies, identification of genes associated with probiotic activity, antimicrobial gene analysis, and antibiotic resistance gene assessment.

Safety and Efficacy Assessment of Probiotic Pediococcus Strains

  • Safety Assessment of Pediococcus Strains

Our solutions facilitate the evaluation of potential probiotic Pediococcus strains' safety by assessing their hemolytic activity, production of biogenic amines, and antibiotic resistance. Our analysis platform enables the examination of metabolites produced by Pediococcus strains, both at the genetic and phenotypic levels, to determine if they produce harmful substances or display antibiotic resistance. This assessment is crucial in determining the safety of Pediococcus probiotics as feed additives.

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Our in vitro efficacy assays enable the evaluation of potential probiotic Pediococcus strains for their antimicrobial properties against pathogens, antioxidant activity, resistance to simulated digestive conditions, including acid and bile salts, and adhesion ability. These solutions are designed to help accelerate the development of Pediococcus as feed probiotics by assessing the probiotic performance of candidate strains.

To demonstrate the safety and beneficial potential of Pediococcus strains in animals, we provide a wide range of animal models for studying the effects of Pediococcus probiotics. By analyzing samples such as animal feed, feces, metabolites, surimi, and animal products, we can help you understand the impact of Pediococcus probiotics on factors like digestibility, growth performance, gut health, and product quality. Comparative testing allows us to determine optimal feeding doses, evaluate different forms of Pediococcus probiotics, and gain insights into their mechanisms through omics studies.

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Fig. 2 Some Pediococcus pentosaceus pathways involved in functional compound production (Jiang, et al., 2021)Fig. 2 Some Pediococcus pentosaceus pathways involved in functional compound production2

Probiotic Pediococcus Fermentation Solution

Pediococcus is a significant component of the microbial flora found in various forage crops and influences silage fermentation characteristics alongside other plant-associated LAB. We offer solutions for evaluating the effects of Pediococcus probiotics on fermentation quality and microbial communities during forage fermentation. Additionally, our solutions optimize fermentation media and conditions for the production of Pediococcus probiotics and related antimicrobial products like pediocin.

Pediococcus Probiotics Development

We provide comprehensive quality control throughout the probiotic development process, including safety control during strain development, purity and potency control for in vivo and in vitro studies, fermentation parameter control for probiotic production, and product quality monitoring. Our aim is to ensure the safety, efficacy, and potency of Pediococcus probiotic products. We assist in developing various forms of Pediococcus probiotic products (e.g., liquid, solid, microencapsulation) based on different application scenarios. Through in vitro and in vivo efficacy and safety evaluations, we provide insights into the probiotic effects of these finished products on different animals.

Probiotic Effects of Pediococcus on Animals

Pediococcus has a long history of use in traditional fermented foods and is also employed as a feed probiotic due to its potential to promote gastrointestinal health, immunity, animal performance, product quality, and mitigate the harmful effects of pathogens. Refer to Table. 1 for specific effects of some Pediococcus probiotics on animals.

Table. 1 Effects of some Pediococcus probiotics on animals

Species Animal species Effects
Pediococcus acidilactici Pigs
  • Its application in diets during late gestation and lactation has positive effects on the reproductive performance, intestinal microflora balance and immunity of sows.
  • Influences some gut parameters of fattening pigs, which may improve feed intake and homogeneity of pigs and could modify ammonia emission.
Chicken Increases the density, length and width of intestinal villus, resulting in better performance and meat quality in broilers.
Dogs Can have a positive impact on antioxidant activity, immunoproteins, SCFAs, and gut microbiota in adult beagles.
Aquaculture species Has positive effects on growth indices and non-specific immune system of green terror.
Pediococcus pentosaceus Pigs
  • Can act as probiotics for weaned piglets by inhibiting Escherichia coli and Salmonella enterica.
  • Contributes to enhanced FCR and effectively modulates weaned piglets' inflammation and intestinal microbiota.
Chicken Can restrain the pathogenicity of Salmonella enteritidis and reduce the death rate in S. enteritidis-infected chicken.
Cattle Improves growth performance of Holstein calves.
Aquaculture species Can enhance immunity and promote growth rate of grass carps.

Feed Probiotic Pediococcus Development Workflow

Fig. 3 Feed probiotic Pediococcus development workflow (BioVenic Original)

Why Choose Us?

Technology and Intellectual Resources

We possess internal and external platforms and resources to aid in the development of animal probiotic products through an integrated approach that evaluates various probiotic effects.

Animal-Focused Solutions

Our solutions are tailored specifically for animals, allowing us to assess probiotic effectiveness based on previous research and our expertise in order to develop highly useful Pediococcus probiotic products.

High-Throughput Omics Services

We provide high-throughput omics services at the genomics, proteomics, metabolomics, and microbiomics levels, saving valuable research time and facilitating in vivo and in vitro studies.

BioVenic's comprehensive solutions span multiple aspects of feed probiotic Pediococcus development, including strain development, safety and efficacy assessment, probiotic fermentation and production, quality control, and finished Pediococcus probiotics product development. If you are interested in our solutions, please feel free to contact us. We look forward to collaborating with you.

References

  1. Damayanti, Ema, et al. "Fermentation of amylolytic yeast and lactic acid bacteria to improve the quality of modified cassava." IOP Conference Series: Materials Science and Engineering. Vol. 1011. No. 1. IOP Publishing, 2021. Under Open Access license CC BY 3.0. The image was modified by extracting and using only Part C of the original image.
  2. Jiang, Shiman et al. "Pediococcus pentosaceus, a future additive or probiotic candidate." Microbial cell factories vol. 20,1 45. 16 Feb. 2021. Under Open Access license CC BY 4.0, without modification.
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