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Veterinary Therapeutic Protein Expression
To address the challenges of producing high-quality veterinary therapeutic proteins for preclinical research, BioVenic offers comprehensive solutions and specialized animal-targeted protein expression systems. Our expertise spans the production of cytokines, plasma proteins, and various enzymes. Our team of experts in custom protein expression brings extensive experience in recombinant veterinary therapeutic protein production. We manage every aspect from sequence design to protein characterization, crafting tailored projects that deliver crucial data to inform your decisions as you prepare to scale up your project.
Protein Expression Overview
Our animal-use protein expression services encompass a range of systems including bacterial, yeast, plant, mammalian cell, insect, and cell-free expressions. Each method is tailored to specific preclinical research requirements, providing options for high-yield production, necessary post-translational modifications, and scalability. This comprehensive suite ensures precise protein expression suitable for various applications in veterinary medicine.
Fig.1 The overview of veterinary therapeutic protein expression service. (BioVenic Original)
Protein Expression Service
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Bacterial Expression System
E. coliremains a top choice for protein expression, boasting various strains tailored for this purpose. In veterinary applications, the process involves inserting DNA coding for your candidate protein into a plasmid expression vector, which is then transformed into bacterial cells. These cells propagate, are induced to express the therapeutic protein, and are subsequently lysed to harvest the product. BioVenic offers a comprehensive service for bacterial protein expression, from gene sequence optimization to scaled-up production, focusing on solutions for veterinary therapeutics. -
Yeast Expression System
Yeast systems, particularly S. cerevisiae, are excellent for producing large quantities of recombinant eukaryotic proteins. This organism's reliability stems from its extensive use in genetics and biochemistry research, making it ideal for veterinary therapeutic proteins that require eukaryotic expression systems. BioVenic provides end-to-end services in yeast expression, ensuring high yields of biologically active proteins for animal health applications. -
Plant Expression System
Utilizing plants like maize, tobacco, rice, sugarcane, and potatoes, this system offers a cost-effective method for mass-producing recombinant proteins. Similar to mammalian systems, plant-based expression can achieve complex post-translational modifications essential for many therapeutic proteins used in veterinary medicine. However, due to the biochemical complexity of plant tissues, extraction and purification processes can be intricate and costly. BioVenic specializes in optimizing these processes to enhance yield and purity, specifically tailoring services to meet the needs of veterinary medicine. -
Mammalian Cell Expression System
Ideal for expressing mammalian proteins that require complex post-translational modifications, our mammalian cell systems utilize viral promoters like SV40, CMV, and RSV for high-level expression. Both transient and stable expression methods are available, providing flexibility based on the project's scale and scope. BioVenic focuses on producing high-quality therapeutic proteins for veterinary applications, ensuring functional and bioactive products through meticulous cell line development and optimization. -
Insect Expression System
Insect cells offer a versatile platform for producing complex eukaryotic proteins with accurate post-translational modifications, crucial for advanced veterinary therapeutics. Our services include both baculovirus-infected systems and non-lytic insect cell cultures, tailored to produce high yields of functional proteins for animal health applications. BioVenic's expertise extends to streamlining the scale-up process, ensuring consistent and reliable protein production. -
Cell-free Expression System
Our cell-free systems provide a rapid and flexible platform for synthesizing proteins in vitro, using purified components such as ribosomes, RNA polymerase, and amino acids. This system is particularly advantageous for assembling proteins with modified amino acids, essential for veterinary research and therapeutic applications. Despite its technical challenges and higher costs, BioVenic excels in leveraging this technology to rapidly prototype proteins for specialized applications in animal health, including multi-protein complexes and novel bioactive compounds.
Expression System Comparison
The characteristics of veterinary therapeutic proteins determine the most suitable expression system for their production. It is also crucial to understand the distinct features and capabilities of each expression system. This table highlights the performance of various protein expression systems across several parameters, aiding in the selection process based on specific requirements such as the type of protein, necessary post-translational modifications, cost considerations, turnaround time, and anticipated yield.
| Bacteria | Yeast | Plant | Mammalian Cell | Insect | Cell-free | |
|---|---|---|---|---|---|---|
| Prokaryotic Protein Expression | +++ | + | - | + | + | + |
| Eukaryotic Protein without PTM | +++ | +++ | ++ | +++ | +++ | + |
| Eukaryotic Protein with PTM | - | ++ | +++ | +++ | ++ | + |
| PTM, Folding | + | ++ | +++ | +++ | +++ | + |
| PTM, Glycosylation | - | + | +++ | +++ | ++ | - |
| PTM, Phosphorylation | - | + | ++ | +++ | ++ | - |
| Turnaround Time | Short | Middle | Long | Long | Long | Short |
| Cost | Low | Low | Middle | Middle | Middle | High |
| Yield (mg/L) | Range from 1-5 mg/L with an average level 3 mg/L | Range from 1-1000 mg/L with an average level 50 mg/L | Range from 1-100 mg/L with an average level 20 mg/L | Range from 1-1000 mg/L with an average level 50 mg/L | Range from 1-5 mg/L with an average level 3 mg/L | Range from 0.1-50 mg/L with an average level 5 mg/L |
Service Workflow
BioVenic is a leading provider of recombinant protein expression, tailored specifically for clients engaged in preclinical veterinary research. Our process begins with a thorough consultation to comprehensively understand your unique requirements. We then carefully select the most appropriate expression systems based on the specific properties of your protein candidate. Upon signing the contract, our dedicated team initiates the expression processes, supported by extensive data analysis. We guarantee continuous technical support and customized after-sales services designed for veterinary applications.
Fig. 2 The veterinary therapeutic protein expression service workflow. (BioVenic Original)
Why Choose Us?
High Purity and Consistency
Recombinant DNA technology allows for the production of veterinary proteins in high purity and with consistent quality, reducing the variability often associated with extracting proteins from natural sources.
Scalable Production
Once the initial development phase is completed, recombinant protein production can be scaled up efficiently, allowing for large quantities that are necessary for industrial applications and commercial production.
Customized Service
Veterinary therapeutic proteins can be engineered to have specific properties, such as increased stability, enhanced activity. Our service can produce recombinant therapeutic protein for animal use during preclinical research.
BioVenic leverages these advantages to provide top-tier recombinant protein expression services that meet the specific needs of our clients in veterinary preclinical research. Whether you are developing new therapeutics or enhancing existing treatments, our team is ready to support your project with our expertise and advanced technologies. Contact us today to discuss how our custom solutions can assist in advancing your research, and to inquire about our services tailored just for you.