Animal Whole-Genome Sequencing

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Animal Whole-Genome Sequencing

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Analytical Services Animal Whole-genome Sequencing Services Workflow Applications Sampling Recommendations

BioVenic offers Whole-genome sequencing (WGS) services for animals, ensuring fast processing cycles, high-quality sequencing data, and dependable outcomes. WGS of animals is a widely used technique to sequence an entire animal genome, which helps identify genomic variants such as SNPs, InDel, CNV, and SV. WGS is a highly accurate method for obtaining a complete genetic profile at the single nucleotide level. Our advanced sequencing technology and expert data analysis team guarantee precise and top-notch sequencing results, allowing you to analyze the complete genomic information of the animals and the association accurately and comprehensively between genes and traits.

Analytical Services

Sample QC	DNA amount ≥ 2 μg, DNA concentration ≥ 20 ng/μl, OD260/280 = 1.8~2.0. All DNA samples are verified for purity and quantity.
Sequencing	Depth of coverage ≥ 100 x. More than 80% of bases have quality score ≥ Q30. Both paired-end and mate-pair libraries can be constructed during the library preparation step.
Bioinformatics Analysis	Raw data quality control. Sequencing depth and coverage statistics. Reference genome mapping. SNP/InDel/SV/CNV calling, annotation and statistics. Pathway enrichment analysis. Population genetics analysis. Additional data mining according to your requirements.

Animal Whole-genome Sequencing Services

BioVenic provides high-quality products and professional services for sample and library preparation, sequencing, data quality control, and bioinformatics analysis. Strict quality control processes are employed to ensure the accuracy and reproducibility of the sequencing process. We use advanced sequencing platforms and related technologies to generate high-quality sequencing data. Each stage undergoes a meticulous, scientifically rigorous process to guarantee the production of reliable research results. The general workflow of animal whole-genome sequencing is summarized below.

Workflow of Animal WGS

Fig.1 Workflow of Animal WGS. (BioVenic Original)

Applications

Discovering the molecular mechanism in animal breeding.

Supplying resources to speed up animal genetic development.

Examining animal species origin and evolution on a molecular level.

Identifying shared genetic differences within different animal populations.

Sampling Recommendations

For common animal species, tissues like liver, kidney, and blood should be chosen for sampling.

For rare animal species, ear samples, hair (with roots), and other low-fat content tissues must be provided for sampling.

To reduce the influence of individual differences in subsequent splicing, collect samples from the same individual, if feasible.

If the population is small and individual DNA extraction is not sufficient to meet the requirements, the number of sampled individuals should be minimized while ensuring an adequate analysis volume.

BioVenic offers animal WGS and bioinformatics analysis services quickly and accurately, with distinctive benefits. We follow strict SOPs to guarantee fast project delivery and unparalleled data quality. Moreover, we possess substantial experience in animal breeding, an advanced sequencing platform, and a professional service team. These enable us to offer customized and advanced solutions for your animal WGS project. Please do not hesitate to contact us if you are interested in our services.

Reference

Xia, Xiaoting, et al. "Assessing genomic diversity and signatures of selection in Jiaxian Red cattle using whole-genome sequencing data." BMC genomics 22.1 (2021): 1-11.