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Animal Induced Pluripotent Stem Cell Solution

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iPSC Reprogramming iPSC Culture Development iPSC Engineering iPSC Differentiation iPSC Characterization iPSC Pluripotency Maintenance iPSC Gene Editing Platform iPSC Epigenetic Detection Platform Animal iPSC Applications Why Choose Us?

Animal induced pluripotent stem cells (iPSCs) are valuable research tools for animal breeding, veterinary medicine, cell therapy development, and pharmaceutical research. Starting with the development and optimization of animal stem cell technology, BioVenic offers customized solutions for researchers in cell reprogramming and technology optimization, cell culture system development, cell directed differentiation and maintenance of cell pluripotency. We are committed to providing high quality, high value and high efficiency customized services.

Fig.1 Generation and Differentiation of Animal iPSCs. (BioVenic Original)Fig.1 Generation and Differentiation of Animal Induced Pluripotent Stem Cells.

iPSC Reprogramming

BioVenic offers one-stop customized services ranging from reprogramming to iPSC proliferation, validation and differentiation, with a special focus on complex reprogramming technologies. Utilizing our state-of-the-art integrated animal stem cell technology and cell detection and culture program development services, we offer clients a range of reprogramming solution design and development services along with rigorously optimized workflows. We also employ a variety of technical methods to regulate the cell reprogramming process to enhance work efficiency and stability.

iPSC Culture Development

BioVenic conducts in-depth research on culture media components, growth factors, and extracellular matrices to provide researchers worldwide with advanced and reliable culture solutions for animal iPSC cells. We support researchers worldwide by providing qualified, high-quality culture systems to generate and expand iPSCs in vitro, maintain cell viability, and direct cell differentiation. With our project experience in the development of animal cell culture systems, we optimize the culture medium formulation and the in vitro culture process for our customers. In addition, we perform strict quality control of the culture system.

Culture System Features
Feeder-dependent
  • Nutritious and reliable.
  • Stably supports PSC growth and maintains pluripotency.
  • Suitable for basic research projects.
  • Animal origin may carry foreign substances.
  • The workflow is cumbersome, and the passage time is long.
  • Trophoblast cells need to be obtained and prepared.
  • Suitable for small-scale operations.
Feeder-free
  • No need to prepare and remove trophoblast cells.
  • Suitable for large-scale culture and high-throughput experiments.
  • More stable performance.

iPSC Engineering

BioVenic focuses on core transformation technologies related to animal induced pluripotent stem cells and provides customized solutions to improve cell transfection engineering and expand the application of iPSCs in research. Our technical team designs and formulates strategies to advance the application of iPSCs in cell therapy research and animal disease model construction from two key perspectives: pluripotent stem cell line construction and CRISPR gene editing.

iPSC Differentiation

BioVenic has extensive project experience in directing the differentiation of animal stem cells and offers a variety of stem cell directed differentiation services, including iPSC. We induce iPSCs to differentiate into target cells according to customer requirements and provide high quality customized solutions. The technical solutions are relatively stable and reproducible.

iPSC Characterization

BioVenic has developed exclusive cell detection and evaluation solutions to determine whether animal cell lines are truly pluripotent stem cells or to test somatic cells of different lineages after differentiation. This ensures that their pluripotency remains intact or that their differentiation is complete. We are committed to using immunoassays and high-throughput platforms to comprehensively evaluate and characterize cells, minimize the use of cells that may have genetic or epigenetic abnormalities in research projects, and provide a solid foundation for generating reliable data for customers' subsequent experiments.

iPSC Pluripotency Maintenance

BioVenic customizes the development of pluripotency factors for the maintenance of animal iPSC pluripotency. Our team of animal cell experts conducts extensive research and analysis of the pluripotency gene regulatory network of mammalian species. We develop effective solutions to maintain cell pluripotency with pluripotency regulators at the core.

iPSC Gene Editing Platform

Keeping pace with the latest research trends, BioVenic provides Cas9 engineering for animal iPSCs to develop a specialized genome editing transformation technology platform. Through the Cas9 method, we can easily introduce mutations that mimic disease occurrence into iPSCs and repair mutations in iPSC disease models. At the same time, genome editing can reduce the immunogenicity of the cells themselves. Our genome editing technology adopts a strategy to maximize the efficiency of genome editing through multiple optimizations and improvements. We are continuously improving the efficiency of gene cutting and controlling homologous recombination. With many years of experience in animal stem cell gene editing, BioVenic provides customized solutions for knockout, knock-in and point mutations of iPSC genomes to ensure the delivery of high-quality iPSC gene-edited cell lines. Our laboratory ensures high transfection efficiency through a strict standardized quality control system. We combine monoclonal identification technology with high-throughput screening and utilize a multi-strategy optimized animal iPSC gene editing platform to ensure the delivery of fully verified positive clone results.

Improvement Direction Strategy
Protocol Design
  • Knockout projects involve selecting single guide RNA (sgRNA) with high specificity and a low off-target rate.
  • For point mutation or gene knock-in projects, it is important to consider the distance between the cutting position and the mutation/knock-in position, the modification of the PAM position, and the design and modification of the homology arm, among other factors.
Transfection Rate
  • Customize transfection methods and parameters for iPSCs from different individuals and tissues.
  • Test and optimize transfection conditions through preliminary experiments.
HDR Efficiency
  • Include resistance genes on the editing plasmid or donor DNA.
  • Combine positive selection marker genes with selectable marker genes that allow excision.
  • Use single-stranded oligonucleotide (ssODN) donor molecules.
  • Use small molecule compounds to inhibit non-homologous end-joining.
Genotype Analysis
  • Developed the genotype analysis system for high-throughput automated screening of positive clones.

iPSC Epigenetic Detection Platform

BioVenic has developed a technical platform for researchers to systematically detect induced iPSCs. We use embryonic stem cells of the appropriate species as reference standards to systematically detect iPSCs at the molecular, cellular and individual animal levels. Our epigenetic detection platform ensures that customers have a comprehensive understanding of the clear genetic background, pluripotency and differentiation potential of the target iPSCs. This helps to ensure the smooth progress of downstream experiments.

Direction Content Technology
Molecular Level
  • The silencing of exogenous gene expression occurs as reprogramming is completed.
  • The activation of endogenous pluripotency gene expression.
  • The genetic background of iPSC.
  • The activity of telomerase
  • The methylation level of pluripotency gene promoters.
  • The overall gene expression level.
  • RT-PCR
  • Microsatellite Fingerprinting
  • Gene Chip
Cell Level
  • Multidirectional differentiation potential.
  • In vitro self-renewal capacity.
  • Cell morphology and surface markers.
  • Cell karyotype detection.
  • AP Staining
  • Immunofluorescence Staining
  • Flow Cytometry
Individual Animal Level
  • In vivo multi-lineage differentiation ability.
  • Tumor Formation Experiment

Animal iPSC Applications

  • Animal Disease Model Construction

The use of iPSCs to generate animal disease models facilitates the study of disease pathogenesis and the development of treatment strategies.

  • Cellular Agriculture

In vitro meat production using animal iPSCs has been proposed as a clean and superior alternative, alleviating environmental stress and reducing human dependence on animal husbandry.

  • Genetic Resource Protection

Differentiation of animal iPSCs to obtain fully functional sperm and oocytes advances the conservation of endangered species and enhances breeding and genomic selection programs in farm animals.

Why Choose Us?

BioVenic has extensive project experience in the field of animal stem cell technology and is proficient in designing technical solutions related to iPSCs and their implementation.

BioVenic offers one-stop solutions for researchers using iPSCs and related technologies, covering the processes of generation, culture, maintenance and directed differentiation.

BioVenic combines various technical platforms and expertise in areas such as animal cell culture, cell detection, and genome editing to ensure the scientific rigor and practicality of experimental solutions.

BioVenic has many years of experience in providing scientific research services in the field of animal stem cells, ensuring that clients' induced pluripotent stem cell related issues are effectively addressed. Our customized solutions cover all areas related to animal pluripotent cells. Please contact us to discuss your research needs related to animal iPSCs.

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