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Animal Induced Pluripotent Stem Cell Reprogramming Regulation

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Epigenetics and Reprogramming Small Molecules and Reprogramming Animal iPSC Epigenetic Detection Why Choose Us?

The fields of animal induced pluripotent stem cells (iPSCs) and epigenetics are rapidly evolving and are continuously improving researchers' understanding of cell reprogramming and differentiation. Different methods of animal cell reprogramming affect the epigenetic status and pluripotency of iPSCs to varying degrees. Animal iPSCs generated by complete chemical reprogramming technology show reduced epigenetic memory and increased differentiation potential compared to cells reprogrammed using the four transcription factors OSKM. BioVenic explores the mechanisms and consequences of epigenetic memory in animal iPSCs from different species, cell types and tissues. It is used to improve and optimize cell reprogramming protocols. To improve the safety, efficiency, and stability of cell reprogramming technology processes and services, we are integrating genomic, epigenomic, and transcriptomic analyses with cell reprogramming technology. This integration aims to establish more standardized programs for generating and maintaining iPSCs by controlling the effects of key signaling pathways, epigenetic modifications, and small RNAs.

Fig. 1 Generation, Expansion, and Characterization of iPSCs in Animals. (Barrachina, et al., 2023)Fig. 1 Generation, Expansion, and Characterization of iPSCs in Companion Animals.1,2

Epigenetics and Reprogramming

BioVenic leverages the critical role of epigenetics in maintaining the pluripotency of animal stem cells and the significant impact of epigenetic modifications on iPSC reprogramming to control the cell reprogramming process. The efficiency of animal iPSC reprogramming can be compromised by incomplete inhibition of differentiation-related transcription factors and insufficient DNA demethylation during reprogramming. Our experimental team is optimizing these epigenetic-related features at the mechanism level to increase the efficiency of reprogramming. At the same time, we pay special attention to the epigenetic changes of iPSCs to reduce the tumorigenicity caused by uncontrolled pluripotency.

Fig. 2 Epigenetic Regulation in Animal Cells. (BioVenic Original)Fig. 2 Animal Cell Epigenetic Regulation.

Small Molecules and Reprogramming

BioVenic has synthesized the significant effects of small molecules on the animal iPSC reprogramming process, providing customers with reliable options for regulating and optimizing solutions to achieve efficient and safe iPSC generation. These small molecules include RNA molecules and compounds that typically target key pathways of epigenetic regulation. They play a critical role in regulating stem cell development and differentiation and directly contribute to maintaining stem cell pluripotency. We are committed to developing a comprehensive solution for the simple and efficient generation of animal iPS cell lines with multidirectional differentiation capabilities by combining small molecules and RNA.

Animal iPSC Epigenetic Detection

BioVenic has developed a technical service for researchers to systematically detect induced iPSCs. We use embryonic stem cells of the appropriate species as reference standards to systematically detect iPSCs at the molecular, cellular and individual animal level. We not only characterize the expression of marker genes specific to pluripotent cells, but also assess the potential for differentiation into the three germ layers and the ability to self-renew. Our epigenetic detection service ensures that customers have a comprehensive understanding of the clear genetic background, pluripotency and differentiation potential of the target iPSCs. This helps to ensure the smooth progress of downstream experiments.

Direction Content Technology
Molecular Level
  • Silencing of exogenous gene expression occurs when reprogramming is complete.
  • Activation of endogenous pluripotency gene expression.
  • The genetic background of the iPSC.
  • Telomerase activity.
  • The level of methylation of pluripotency gene promoters.
  • The total gene expression level.
  • RT-PCR
  • Microsatellite Fingerprinting
  • Gene Chip
Cell Level
  • Multidirectional differentiation potential.
  • In vitro self-renewal capacity.
  • Cell morphology and surface markers.
  • Cell karyotype detection.
  • AP Staining
  • Immunofluorescence Staining
  • Flow Cytometry
Individual Animal Level
  • In vivo multilineage differentiation potential.
  • Tumor Formation Experiment

Why Choose Us?

BioVenic has a strong biological background and offers comprehensive services and state-of-the-art technology platforms for animal metabolomics, cell biology, stem cell research and cell culture system development.

BioVenic continues to standardize the technical process of cell reprogramming while improving specific reprogramming solutions for cells from different species and tissues.

BioVenic's team of experts from various fields of animal cell biology thoroughly discusses solutions for each service and provides ongoing technical support.

BioVenic's extensive experience in the field of animal stem cells enables us to provide our customers with comprehensive and high-quality customized solutions. We specialize in basic biological technologies and cutting-edge high-throughput detection technologies to address our customers' scientific research challenges related to the regulation of animal induced pluripotent stem cell reprogramming. To regulate the reprogramming process of animal iPSCs, we take an epigenetic approach and create high-quality experimental solutions that are efficient and conducive to maintaining pluripotency. If you are looking for strategies to improve reprogramming efficiency or to detect generated iPSCs, please contact us for more information.

References

  1. Image retrieved from Figure 1 "Overview of the process for generating induced pluripotent stem cells (iPSCs) from companion animals." Barrachina et al., 2023, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). The original title was changed to "Generation, Expansion, and Characterization of iPSCs in Companion Animals."
  2. Barrachina, Laura, et al. "Induced pluripotent stem cells in companion animals: how can we move the field forward?" Frontiers in Veterinary Science 10 (2023): 1176772.
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