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Animal DNA Methylation Analysis
DNA Methylation Analysis Services
DNA methylation is one of the most extensively researched epigenetic modifications and plays a critical role in maintaining the stability of the animal genome and ensuring normal growth and development. Understanding the dynamics of DNA methylation is essential for studying animal spermatogenesis, embryonic development, disease resistance, stress resistance, and the development of economic traits such as muscle and fat. It can also serve as a significant theoretical basis for breeding development. BioVenic offers comprehensive and reliable analysis solutions for DNA methylation status, enrichment, and sequencing to global scientific researchers. Our services encompass methylation levels from single base to genome-wide, addressing various challenges encountered in related research.
Fig.1 DNA Methylation and Demethylation in Cattle. 1
DNA Methylation Analysis Services
- Targeted Bisulfite Sequencing
BioVenic provides targeted bisulfite sequencing service for evaluating site-specific DNA methylation changes. Our comprehensive project approach enables customers to achieve single-base resolution methylation analysis. This includes target region processing and PCR amplification, library construction, and sequencing of amplicon regions. With high sequencing coverage depth for every DNA methylation site, BioVenic simplifies the process for customers to acquire precise, quantitative, and single-base resolution data output.
- Whole Genome Bisulfite Sequencing (WGBS)
Whole-genome bisulfite sequencing (WGBS) service combines bisulfite processing and high-throughput sequencing to identify methylated CpG sites, with a coverage rate of 90%. We offer WGBS-based DNA methylation analysis solutions for scientific research projects investigating animal gene expression and epigenetic regulation. BioVenic enables you to easily explore and complete tasks such as creating single-base resolution whole-genome DNA methylation maps, conducting disease association analysis, analyzing gene regulation, and screening for disease markers.
- Reduced Representation Bisulfite Sequencing (RRBS)
BioVenic's Reduced Representation Bisulfite Sequencing (RRBS) platform is built on an enhanced bisulfite sequencing method and encompasses nearly all promoters, CpG islands, gene bodies, repetitive DNA sequences, and regulatory elements within animal genomes. RRBS reduces the complexity of the genome by combining bisulfite sequencing with restriction enzymes, enabling more targeted analysis of genomic regions with high CpG content. We utilize RRBS technology to tailor precise and efficient DNA methylation research methods for scientific researchers. This ensures high-resolution detection of DNA methylation status and enhances the efficient utilization of sequencing data. BioVenic relies on an experienced and professional technical team to provide comprehensive whole-genome methylation mapping analysis services, integrating NGS technology, bisulfite sequencing, and restriction endonuclease MspI to deliver reliable results and a comprehensive project plan.
Workflow
Global Quantification
BioVenic offers quantitative detection and analysis services for global DNA methylation levels to determine the status in different cell types and regions of the animal genome. We utilize 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) to accurately measure DNA methylation across the entire genome, offering precise data for research areas such as disease mechanisms and gene expression regulation.
| 5-mC DNA | During DNA methylation, DNA methyltransferase covalently attaches a methyl group to the 5th carbon atom of cytosine, forming 5-methylcytosine (5-mC), which can be utilized to measure the DNA methylation level of a single gene. |
| 5-hmC DNA | 5-Methylcytosine (5-mC) is oxidized by the TET protein family to produce 5-hydroxymethylcytosine (5-hmC), thereby completing the demethylation process of DNA cytosine. 5-hmC can be used to measure the levels of DNA hydroxy-methylation in individual genes. |
Why Choose Us?
Team of experienced experts in animal epigenetics selects the most suitable technology platform and tailors a comprehensive implementation plan to align with your research objectives.
Utilize advanced technologies, including methylation sequencing platform, methylation-specific PCR platform, 5-hmC and 5-mC quantification, to generate biological information effectively and efficiently about the methylation status of individual cytosine.
From sample processing to obtaining the final data report, rigorous QC audits are conducted at every stage to fundamentally ensure high-quality and reliable data output.
Comprehensive and rigorous bioinformatics data analysis involves detecting and calculating methylation levels, analyzing the frequency distribution of methylation levels, and detecting and annotating differential methylation.
Animal DNA methylation is one of the most distinctive epigenetic modifications and is involved in a variety of biological processes, including the silencing of transposable elements, regulation of gene expression, and genomic imprinting. BioVenic provides comprehensive coverage of DNA methylation-related technology platforms, offering professional project solutions and an experienced team of experts to support and safeguard customers' research projects. If you have any scientific research needs related to DNA methylation analysis, please feel free to contact us to receive a highly customized and reliable one-stop solution.
Reference
- Zhang, Junxing, et al. "Effects of DNA methylation on gene expression and phenotypic traits in cattle: A review." International Journal of Molecular Sciences 24.15 (2023): 11882.