This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Animal mRNA Sequencing
Animal mRNA Sequencing Services
Animal RNA sequencing (RNA-Seq) is revolutionizing transcriptome research as a highly sensitive and accurate tool for detecting whole-transcriptome expression. BioVenic uses animal mRNA sequencing technology to detect previously undetectable changes in animal disease states, growth and development, trait expression, under various environmental conditions, and in a variety of other study designs. Our service utilizes distinct technical strategies for various sample types, enabling the detection of not only standard samples but also degraded and trace samples. This enables the detection of transcript isoforms, gene fusions, single nucleotide site variations, and other features without prior knowledge. Customers can flexibly choose customized mRNA sequencing service plans based on sample conditions.
Fig.1 Utilizing RNA-Seq Technology to Analyze Differential Gene Expression in Cattle Tissues. 1
Animal mRNA Sequencing Services
- Extraction of Total RNA
BioVenic has developed a mature and comprehensive standardized process for total RNA extraction, which is supported by rigorous quality checks throughout the extraction process, including purity, concentration, and integrity (RIN).
- mRNA Capture and Fragmentation
The animal mRNA sequencing service captures the mRNA transcribed by specific cells or tissues of animal samples in a certain functional state for customers. It utilizes oligo-dT magnetic beads to capture RNA with a PolyA tail for library sequencing, a method also known as PolyA-Seq.
- cDNA Synthesis and Library Construction
BioVenic combines gene synthesis, sequencing, and PCR platforms to ensure that the entire process is rigorous, reliable, and allows for easy construction of mRNA libraries.
- In Silico Sequencing and Data Analysis
BioVenic relies on its high-throughput sequencing platform to efficiently compare and sequence libraries, while simultaneously conducting quality control and filtering of sequencing data. BioVenic offers a variety of custom bioinformatics analyses.
Animal mRNA Sequencing Service Flow Chart
RNA-Seq Analysis Platforms
BioVenic's RNA sequencing (RNA-Seq) platform is tailored for scientific users who need detailed analysis of the influence of RNA on gene expression and cellular function in animal samples. The platform utilizes next-generation sequencing to capture snapshots of animal transcriptomes and identify non-transcriptomic RNA sequences. At the same time, RNA-Seq can achieve quantification and identify differential expression, providing a powerful tool for in-depth exploration of animal physiological health conditions and transcriptome regulation of various common diseases. With this platform you can easily achieve:
RNA-Seq Analysis Workflow
Why Choose Us?
One-stop Service
Customers only need to provide cells or tissues, and BioVenic can complete the entire service process from sample preparation and library preparation to computer sequencing and data analysis.
Excellent Sample Handling
BioVenic utilizes a distinctive rRNA removal kit designed for degraded samples. This kit can effectively preserve mRNA information in degraded samples, making it particularly well-suited for mRNA detection in clinical samples.
Rich Signaling Pathway Database
BioVenic provides comprehensive annotations on gene targets associated with prevalent diseases in animals and the signaling pathways linked to these genes. It can conduct more personalized integration and screening based on the mRNA sequencing data and the customer's research field.
Strong and Professional Technical Team
BioVenic conducts strict quality control on all aspects of the experiment to minimize experimental risks as much as possible. BioVenic has a robust bioinformatics team capable of addressing customers' diverse and in-depth data analysis requirements.
BioVenic's animal mRNA-Seq technology services not only offer precise and sensitive quantitative detection of gene expression but also facilitate the identification of both known and novel transcript isoforms, gene fusions, and other characteristics, including allele-specific expression. BioVenic provides global scientific researchers with comprehensive and rapid access to nearly all mRNA sequence information of a specific animal tissue or organ of a species in a particular state. We also analyze and compares the differences between multiple animal samples. Please feel free to contact us for highly customized and reliable animal transcriptome sequencing analysis solutions.
Reference
- Fang, Xibi, et al. "Comprehensive Analysis of miRNAs and Target mRNAs between Immature and Mature Testis Tissue in Chinese Red Steppes Cattle." Animals 11.11 (2021): 3024.