Animal Non-coding RNA Analysis

Animal Non-coding RNA Analysis Services

The portion of total RNA that remains after removing mRNA is collectively referred to as non-coding RNA. This type of RNA plays a role at both the transcriptional and post-transcriptional levels and contributes significantly to the epigenetic regulation of animal gene expression. BioVenic offers customized solutions for animal non-coding RNA sequencing and analysis, delving deeply into a range of important biological functions carried out by ncRNA. By utilizing next-generation sequencing platforms and PCR amplification for library construction and comparison, as well as conducting real-time quality assessment of samples, libraries, and sequencing data, we can analyze the biological information in various types of non-coding RNA (ncRNA) and provide dependable project results for our clients.

Fig.1 Schematic Diagram of the Conformation and Conservation of LncRNA in Organisms. ¹

Animal Non-coding RNA Analysis Services

• RNA Enrichment

BioVenic offers customers sample pretreatment and target RNA enrichment solutions. Ribosomal RNA (rRNA) accounts for over 90% of cellular RNA. We employ specialized purification and enrichment technology to eliminate rRNA and/or utilize oligo (dT) primers for Poly(A) selection on mRNA, ensuring accurate capture of the RNA from the sample. The specificity of the RNA type of interest and the accuracy of the sequencing process ensure that the data is free from biased errors.

• RNA-Seq Library Construction

The primary distinction between RNA sequencing library and DNA sample library construction workflows is the requirement to reverse-transcribe RNA into cDNA. We utilize our years of project experience to accurately capture all types and sizes of RNA species, ensuring the diversity and high quality of your RNA libraries. We offer our customers comprehensive services, including cDNA synthesis, purification, library preparation, and the removal of ribosomal RNA (rRNA).

• Sequencing and Quantitation

BioVenic's next-generation sequencing library enables precise quantification of specific types of RNA or global non-coding RNA in samples. This provides customers with a powerful technical platform for in-depth and high-precision analysis of biological information in data.

• Data Comprehensive Analysis and Interpretation

BioVenic has a professional team of cross-disciplinary experts with expertise in various fields of zoology. They specialize in providing comprehensive and detailed analysis of high-throughput detection data. At the same time, we have developed exclusive quality inspection tools to monitor the quality and standardization of reading and analysis, and to achieve quality control of detection data by eliminating contaminating errors.

Fig.2 Classification of Animal Non-coding RNAs.

Why Choose Us?

BioVenic has extensive experience and cutting-edge scientific research technology platforms in projects related to animal epigenetics and RNA detection and library design components. You only need to mention your interest in non-coding RNA, and our professional team will tailor a reliable and effective experimental plan for you, and a multi-field cross-expert team will transform the data into advanced bioinformatics analysis directions. Our data analysis services are comprehensive and detailed, covering functional site identification, differential analysis, functional enrichment, and interactive visualization. If you have any confusion about analyzing animal non-coding RNA, please contact us to facilitate the progress of your project.

Reference

1. Ramírez-Colmenero, América, Katarzyna Oktaba, and Selene L. Fernandez-Valverde. "Evolution of genome-organizing long non-coding RNAs in metazoans." Frontiers in Genetics 11 (2020): 589697.