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Animal Chromatin Accessibility Analysis
Animal Chromatin Accessibility Analysis Services
Regulation of gene expression can be achieved by altering the chromatin topology or making chromatin modifications. Chromatin accessibility patterns change dynamically in response to external stimuli and developmental cues. This dynamic remodeling is closely related to the animal's growth and development, trait expression, disease occurrence, immunity, and cell fate determination. BioVenic has launched a one-stop service for animal chromatin accessibility analysis, offering animal research on chromatin accessibility for customers and conducting three-dimensional analysis of chromatin functions. We utilize high-throughput sequencing technology and a range of optional detection methods to address diverse scientific research requirements and comprehensively analyze the biological information encompassed in dynamic modifications and structural changes of chromatin.
Fig.1 Comprehensive Analysis of Porcine Chromatin Accessibility Across Multiple Tissues and Developmental Stages. 1
Animal Chromatin Accessibility Analysis Services
BioVenic has developed comprehensive and diverse platforms for analyzing animal chromatin accessibility. This Service offers scientific researchers a reliable option for studying the relationship between chromatin structure and function at various developmental stages or positions, addressing the research needs in the field of animal epigenetics.
- MNase-seq Chromatin Accessibility Analysis
BioVenic offers micrococcal nuclease sequencing (MNase-seq) for chromatin accessibility analysis. MNase is used to digest the DNA regions exposed between nucleosomes, releasing nucleosomes and enriching DNA fragments for sequencing. This technical service indirectly assesses the accessible regions of animal chromatin and subsequently analyzes the functional genome.
- DNase-seq Chromatin Accessibility Analysis
BioVenic provides deoxyribonuclease hypersensitive site sequencing (DNase-seq) services for chromatin accessibility analysis. We utilize the endonuclease DNase-I to non-specifically cleave genomic DNA and enrich DNA fragments for sequencing.
- FAIRE-seq Chromatin Accessibility Analysis
BioVenic provides formaldehyde-assisted separation of regulatory element sequencing (FAIRE-seq) for chromatin accessibility analysis services. We covalently bound animal genomic DNA to chromatin proteins using the cross-linking agent formaldehyde, and then fragmented it with ultrasonic waves. After the organic extraction, the DNA was sequenced and analyzed.
- ATAC-seq Chromatin Accessibility Analysis
BioVenic offers high-throughput sequencing chromatin accessibility analysis services for Assay for Transposase-Accessible Chromatin (ATAC). We inserted sequencing adapters into open regions of chromatin using highly active Tn5 transposase, and then utilized sequencing data to deduce the accessibility of chromatin regions in animal samples.
Animal Chromatin Accessibility Analysis Platforms
| Platforms | Technical Principles | Features |
| MNase-seq | MNase preferentially digests nucleosome-free DNA. |
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| DNase-seq | DNase I cleaves DNA sequences not protected by proteins. |
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| FAIRE-seq | Ultrasound breaks the chromatin that cross-links genomic DNA. |
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| ATAC-seq | Tn5 transposase simultaneously cleaves the open region DNA and inserts the sequencing adapter. |
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Animal Chromatin Accessibility Analysis Workflow
Why Choose Us?
Diverse Solution Design
We have mastered a variety of techniques for detecting animal chromatin accessibility and have established mature project processes and experimental platforms to fully meet your personalized research needs.
High-Quality Data Analysis
We conduct quality control and preprocessing in the project analysis process, followed by clustering and differential analysis of open chromatin regions. This approach ensures reliable functional annotation and enrichment analysis of gene targets, as well as the identification of key regulatory elements.
Optimized Detection Technology
BioVenic leverages its extensive project experience to optimize chromatin accessibility detection technology, reducing sample requirements and enhancing accuracy.
BioVenic is dedicated to offering comprehensive analysis solutions for chromatin accessibility to researchers studying animal genetics, enabling them to fully interpret the biological information within. We utilize our cutting-edge technology platform, personalized solution design, and professional project interpretation to deliver high-quality and efficient one-stop solutions. If you encounter any challenges with dynamic changes and structural analysis of chromatin, please contact us for assistance with your research. We will be your most reliable scientific research partner.
Reference
- Bai, Jingyi, et al. "Profiling of chromatin accessibility in pigs across multiple tissues and developmental stages." International Journal of Molecular Sciences 24.13 (2023): 11076.