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Veterinary Therapeutic Protein and Peptide Sequence Analysis
Each protein and peptide has its unique number and sequence of amino acid residues, which determine their specific higher-order structures through folding, thereby influencing their biological functions. BioVenic offers sequence analysis services for veterinary therapeutic proteins and peptides providing detailed elucidation for the identification of these proteins and peptides by precisely analyzing these sequences, aiding in novel veterinary therapeutic protein and peptide preclinical research.
Protein and Peptide Sequence Overview
Veterinary therapeutic protein and peptide sequence analysis is essential for elucidating the amino acid sequence and understanding the functional attributes of therapeutic agents. At BioVenic, we employ cutting-edge techniques to provide comprehensive sequence analysis, including N-terminal sequencing, C-terminal sequencing, peptide mapping, peptide mass fingerprinting, amino acids composition and de novo sequencing, and sequence alignment. Our advanced services help in verifying the identity of proteins and peptides and ensuring consistency across batches. By leveraging high-resolution mass spectrometry and bioinformatics tools, we deliver precise and actionable insights that support the development, optimization, and quality control of veterinary therapeutics.
Fig.1 The protein and peptide sequence analysis service BioVenic provided. (BioVenic Original)
Protein and Peptide Sequencing Service
- N-terminal Sequencing
The N-terminal sequencing of veterinary therapeutic proteins and peptides determines the N-terminal amino acid residues of the sample. The biosynthesis of proteins and peptides starts at the N-terminus, and the composition of their N-terminal sequence greatly influences their biological functions. The N-terminus undergoes various post-translational modifications, which are closely related to the protein's function and stability. BioVenic offers N-terminal sequencing services for veterinary therapeutic proteins and peptides based on Edman degradation and mass spectrometry analysis. Edman sequencing accurately analyzes N-terminal sequences, while mass spectrometry addresses limitations related to N-terminal blockage and post-translational modifications. These methods complement each other, providing comprehensive analysis without restricting sample types.
| Services | Procedure | Sample Requirements |
|---|---|---|
| Edman Degradation |
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| Mass Spectrometry Analysis |
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- C-terminal Sequencing
Protein synthesis in living organisms always proceeds from the N-terminus to the C-terminus. As the termination of synthesis, the C-terminus contains rich information related to biological functions and plays an important role in protein localization, protein recognition signaling, etc. BioVeinc provides C-terminal sequencing services for veterinary therapeutic proteins and peptides, relying on mass spectrometry platforms, to provide sequence identification and analytical information on the C-terminus of proteins and peptide molecules.
| Services | Procedure | Sample Requirements |
|---|---|---|
| Carboxypeptidase Method |
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| Top-Down Sequencing |
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- Peptide Mapping
Peptide mapping is a critical step in the characterization process of veterinary therapeutic proteins and peptides. It involves enzymatically breaking down proteins or peptides into their constituent peptide fragments. BioVeinc offers peptide mapping services for veterinary therapeutic proteins and peptides, utilizing a "bottom-up" characterization strategy to achieve comprehensive coverage of the sample's complete sequence, ensuring the integrity and consistency of its structure and function.
| Sample Requirements | ||
|---|---|---|
| In-gel and solution samples are available | Purity>80% | Quantity 10-20μg |
Fig.2 The peptide mapping service workflow. (BioVenic Original)
- Peptide Mass Fingerprinting
Peptide mass fingerprinting (PMF) analysis is used for identifying the identity of proteins. Its principle is based on the capability of mass spectrometry to accurately measure the mass-to-charge ratio (m/z) of peptide fragments. The mass of each peptide fragment is unique, depending on its amino acid composition and sequence. By analyzing these mass data, a unique "fingerprint" of a specific protein is constructed. BioVeinc offers peptide mass fingerprinting analysis services for veterinary therapeutic proteins and peptides, identifying the protein's identity by comparing the peptide mass data obtained from experiments with the theoretical peptide masses of known proteins in the database.
Fig.3 The peptide mass fingerprinting service workflow. (BioVenic Original)
- Amino Acids Composition Analysis
Amino acid analysis is used to determine the amino acid content in peptide and protein samples. Proteins and peptides are long linear polymers of amino acids linked by peptide bonds, and the first step in amino acid analysis is the hydrolysis of these peptide bonds. During hydrolysis, the peptide bonds in the protein are broken, releasing individual amino acids, which are then introduced into a chromatography system for separation BioVeinc offers veterinary therapeutic protein and peptide amino acid composition analysis services. Relying on the LC-MS/MS system, each amino acid is detected and identified based on its specific retention time. By comparing the amino acid composition of the sample with known standard compositions, the structural information of the protein or peptide is inferred.
| Sample Requirements | ||
|---|---|---|
| Dry powder state | Quantity>3μg | |
| Avoid high salt concentrations | No Tris buffer system | |
Fig.4 Schematic diagram of Amino acid analysis. (BioVenic Original)
- De novo Sequencing
De novo sequencing refers to the direct determination of the amino acid sequence of proteins or peptides without any prior knowledge of the sequence. BioVeinc offers de novo sequencing services for veterinary therapeutic proteins and peptides, relying on ESI and MALDI mass spectrometry systems to provide detailed information about new or variant proteins and peptides.
Service Workflow
BioVenic provides a comprehensive range of veterinary therapeutic protein and peptide sequence analysis services designed to meet the specific needs of our clients. Our streamlined workflow ensures precision and efficiency at every step. Start by sending us your inquiry, and our team of experts will collaborate with you to design a customized experiment tailored to your requirements. Upon agreement and contract signing, we commence the project, applying our advanced technologies and methodologies to ensure the highest quality results. The process culminates in the delivery of a detailed and thorough analytic report, providing you with the insights and data needed to advance your research and development efforts. Our commitment to accuracy and excellence ensures that you receive the best possible analysis services, supporting the development of innovative veterinary therapeutics.
Fig.5 The Therapeutic protein and peptide sequence service workflow. (BioVenic Original)
Why Choose Us?
One-stop Service
One-stop service to complete sample processing, on-board analysis, data analysis, and reports.
Advanced Technologies
Equipped with high-resolution mass spectrometry and chromatography to help provide high-quality results.
Customized Service
Our sequence analysis service is tailored to specific needs for veterinary proteins/peptides discovery.
Technical Support
We also provide comprehensive after-sales technical support to assist clients in understanding their data.
BioVeinc provides a range of sequence analysis services for veterinary therapeutic proteins and peptides utilizing advanced technologies such as mass spectrometry and bioinformatics tools. Our services are aimed at accurately determining the amino acid sequences of proteins and peptides, gaining a deep understanding of their structure and function, thereby supporting the development and optimization of veterinary therapeutic drugs. If you have related needs, please do not hesitate to contact us now.