Animal Nucleic Acid Extraction and Purification

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Animal Nucleic Acid Extraction and Purification

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Services Workflow Platforms Applications Why Choose Us?

Nucleic acids carry genetic information and are of great significance in animal development and breeding research. BioVenic has long been proficient in the extraction and purification technology of animal nucleic acids. With advanced technology platforms, standardized experimental procedures, and rich project experience, we offer comprehensive services and technical support managed by a professional team. Additionally, we provide a range of related and efficient test kits.

Fig.1 Schematic Diagram of DNA and RNA.

Animal Nucleic Acid Extraction and Purification Services

We provide one-stop solutions for animal DNA and RNA extraction and purification. Our team of experts selects the appropriate extraction method for your research samples and effectively purifies a wide range of nucleic acids, ensuring the integrity and reliability of the results delivered.

Animal DNA Extraction and Purification

BioVenic has rich experience in DNA purification and analysis projects, providing genomic DNA extraction, plasmid extraction, DNA electrophoresis, DNA quantification and other services. We mainly focus on animals, efficiently extracts DNA from animal tissues, cells, feces, and blood samples, and owns a professional DNA quality testing and analysis platform to ensure the quality of your experiments in all aspects.

Animal RNA Extraction and Purification

BioVenic provides professional services such as total RNA purification, mRNA purification, microRNA purification, and RNA quantification. We have a professional RNA quality detection and analysis platform and a team of experienced experimentalists to provide comprehensive scientific research services. We aim to meet customers' requirements for RNA extraction and further contribute to research.

Workflow for DNA/RNA Extraction and Purification

Fig.2 Schematic Diagram of The Three Methods. (BioVenic Original)

Animal Nucleic Acid Extraction and Purification Technology Platforms

BioVenic comprehensively evaluates the customer's animal sample type, nucleic acid type, sample quantity, extraction efficiency, purity, cost-effectiveness, and other factors to provide the best technical solution for your experiment.

Fig.3 Schematic Diagram of The Three Methods.

Methods	Principles	Steps	Advantages	Limitations
Phenol-Chloroform Extraction	DNA/RNA is easily soluble in water, but insoluble in organic solvents.	Mix equal volumes of phenol and nucleic acid lysis buffer. Layer the solution with the water layer on top and the phenol layer on the bottom. Shake the mixture well, allow it to stand, separate the water layer, and extract DNA/RNA.	Low cost.	Use of toxic reagents, low nucleic acid recovery, poor reproducibility, cumbersome extraction procedure.
Silica Column Chromatography	The membrane or resin material in the spin column has a strong affinity and adsorption force only for nucleic acids. It does not adsorb other biochemical component groups outside the column during centrifugation.	Spin column adsorption of nucleic acids. Elution to remove impurities. Final elution of nucleic acids.	Safe reagent, low reagent consumption, high purity, and structural integrity of the nucleic acid.	Suitable for small numbers of samples, expensive (requires specially formulated bead buffer and specially modified beads).
Magnetic Bead-based Method	Specific binding of nucleic acids to magnetic bead materials.	Magnetic bead activation. Nucleic acid binding. Magnetic separation. Impurity elution. Nucleic acid collection.	High purity, reasonable price, convenient operation, highly regarded by research users.	Extraction volume affected by sample degree of elution affects yield, binding capacity of centrifugal column affects adsorption of nucleic acid.

Applications

Examine the structure and function of animal genome.

Investigate the levels of animal gene expression.

Create recombinant DNA using molecular cloning techniques.

Study animal genes using genetic analysis and genotyping.

Conduct next-generation sequencing (NGS) in animal research.,

Why Choose Us?

We guarantee the integrity of the primary structure of nucleic acid.

Our deliveries strictly exclude contamination by other nucleic acid molecules.

Optimize enzyme-inhibiting organic solvents and excessively high concentrations of metal ions.

We minimize contamination by proteins, polysaccharides, and lipid molecules.

BioVenic's services are optimized to maximize the yield, purity, and integrity of nucleic acid, ensuring their usability in subsequent biological experiments and research projects. We have a team of skilled technical specialists and an advanced technological platform to provide you with comprehensive support and assistance. If you are interested in our services, please do not hesitate to contact us.

Reference

Bo, Ye Yin, et al. "High-purity DNA extraction from animal tissue using picking in the TRIzol-based method." Biotechniques 70.3 (2021): 186-190.