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Animal Chromatin Conformation Analysis
Chromosome Conformation Capture Services
Animal chromatin conformation analysis technology can uncover genome interactions in multiple dimensions and provide crucial information about the influence of chromatin structure on transcriptional regulation. An in-depth understanding of the spatial organization of animal genomes is crucial for comprehending animal gene regulation and epigenomics. BioVenic specializes in developing customized services for chromatin conformation analysis. We focus on studying the spatial position relationship of chromatin DNA within the entire genome by taking the entire nucleus of animal cells as the research object. This involves using high throughput sequencing technology and combining it with biological information analysis methods. We offer customers high-resolution three-dimensional chromatin information by capturing all DNA interaction patterns within chromatin.
Fig.1 The Amplification Link in Chromatin Conformation Capturing Technology. 1
Chromosome Conformation Capture Services
BioVenic offers comprehensive solutions for studying animal chromatin conformation. The chromatin conformation analysis technology platform has developed a comprehensive end-to-end workflow from sample preparation to analysis. We develop thorough sample preparation and customized sequencing protocols and analysis workflows tailored to our customers' samples and project objectives. With optimized numerical lengths and multi-dimensional data processing procedures, we comprehensively analyze conformational information for our customers, and then examine various regulations on cellular life activities.
- Sample Preparation and Chromatin Cross-Linking
BioVenic has established comprehensive and mature standard procedures for processing animal samples to ensure the integrity of the samples to the greatest extent. Our technicians use formaldehyde to crosslink interacting DNA fragments, completing a crucial step in analyzing chromatin conformation.
- Restriction Enzyme Selection
We select the most suitable restriction enzymes based on the specific requirements and target characteristics of our customers. Depending on the genetic locus being analyzed, we add an excess of adapter restriction endonuclease to separate uncross-linked DNA from cross-linked DNA.
- Library Construction and Molecular Probe Design
The construction of a library and the design of molecular probes are crucial intermediate steps in the process of capturing animal chromatin conformation. BioVenic relies on years of project accumulation to meticulously construct libraries using information about targets to be evaluated, ensuring the efficiency and high quality of the entire project.
- High-Order Chromatin Structure Analysis
BioVenic has developed a comprehensive bioinformatics analysis platform. Our animal chromatin conformation capture analysis software enables easy implementation of various experiments to generate statistical analysis of data with diverse structures and statistical properties. It also supports personalized customized analysis directions. We offer clients a comprehensive analysis of genome-wide chromatin organization domains and mapping of high-order three-dimensional spatial structures to facilitate the visualization of large and complex datasets.
Animal Chromatin Conformation Analysis Workflow
Why Choose Us?
BioVenic has extensive experience in analyzing animal chromatin conformation, including processing tissue or cell samples, preparing libraries, and conducting comprehensive biological analyses.
BioVenic specializes in various animal epigenetic analysis technologies, including but not limited to next generation gene sequencing and mass spectrometry.
BioVenic adheres to stringent quality control principles and conducts multiple, multi-point quality inspections throughout the project to ensure the consistency and stability of the entire analysis process.
BioVenic is dedicated to assisting scientists in gaining a deeper understanding of animal cell nuclear structure, chromatin spatial organization, and the exploration of gene regulatory mechanisms. At the same time, our personalized and customized services can meet the diverse scientific research fully needs of various customers possible. If you are conducting research in the field of animal genetics and epigenetics, please contact us to integrate animal chromatin conformation analysis technology into your scientific research tools and results.
Reference
- Hagege, Helene, et al. "Quantitative analysis of chromosome conformation capture assays (3C-qPCR)." Nature protocols 2.7 (2007): 1722-1733.