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Animal Nutrition and Metabolism Analysis

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Analysis Services Colorimetric Assays Immunoassays Chromatography Analysis Mass Spectrometry (MS) NMR FT-IR Raman Spectroscopy Metabolomics Applications Workflow Advantages

Together with our enthusiastic team and state-of-the-art testing platforms, BioVenic is ready to provide you with our one-stop animal nutrition and metabolism analysis services, including colorimetric assays, immunoassays, chromatography analysis, mass spectrometry analysis, metabolomics analysis, and more. Our nutritional metabolite analysis services will help you gain a better understanding of the mechanism of animal nutritional metabolism, enabling in-depth research into the diagnosis and treatment of animal nutritional metabolic disorders. We offer not only services, but also a complete solution for your specific needs.

Animal Nutrition and Metabolism Analysis Services

Animal nutrition and metabolism analysis is an important section of our Animal Nutrition and Metabolism Solution. BioVenic's technical platform has developed various qualitative or quantitative analysis methods for proteins, oligopeptides, amino acids, bile acids, fatty acids, organic acids, oligosaccharides, oxidants, indoles and other substances in various biological samples such as serum, plasma, tissue, cells and cell culture fluids. We not only detect individual indicators, but also provide you with metabolomics solutions that open up new perspectives for your animal nutrition and metabolism research.

Colorimetric Assays

Colorimetry is a method of determining the content of a component to be measured by comparing or measuring the depth of color of a solution of a colored substance. BioVenic provides quality assured customized colorimetric assay kits and also analytical services using colorimetric assays, which enable researchers to measure thousands of animal nutritional metabolites.

  • Applications: glucose, uric acid, lactic acid, ions, albumin, ammonia-nitrogen, enzymes, etc.
  • Pros: simple, low cost, rapid
  • Cons: interference, high purity of measured components required, low sensitivity

Immunoassays

Our immunological detection methods, mainly including Enzyme-linked Immunosorbent Assay (ELISA) and Lateral Flow (LF) device, are based on the principle of specific binding of antigens and antibodies, which can rapidly detect corresponding substances. We also provide customized development services for species-specific antigens and antibodies according to your needs.

ELISA
  • Applications: large molecules and small molecules.
  • Pros: high sensitivity, qualitative or quantitative, lower cost compared to chromatography.
  • Cons: possibility of false-positive and false-negative results, tedious assay procedure.
LF
  • Applications: hormones, etc.
  • Pros: rapid, simple, economical, lower experimental conditions requirements.
  • Cons: most are qualitative, possibility of false-positive and false-negative results.

Chromatography Analysis

Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. We have established both gas chromatography (GC) and high-pressure liquid chromatography (HPLC) platforms, which can detect animal nutritional metabolites including short chain fatty acids, amino acids and more.

GC
  • Applications: volatile components.
  • Pros: minimal sample preparation, multifaceted, highly sensitive, suitable for the separation of very little amounts of analytes.
  • Cons: not reliable for non-volatile or thermolabile substances.
HPLC
  • Applications: a broader range of compounds than GC, polar and non-volatile substances.
  • Pros: quick, highly reproducible.
  • Cons: require more extensive sample preparation, costly.

Mass Spectrometry (MS)

Our high-resolution MS provides a powerful method for generating hypotheses based on complete metabolic profiles. Coupled high-efficiency separation technique and MS systems is also available, which can enhance the capabilities of each technique synergistically.

GC-MS

A very versatile technique for compounds that are volatile or can be made volatile.

LC-MS

Well suited to metabolomics. Provides good coverage of a wide range of chemicals.

IC-MS

A new tool for the analysis of charged metabolites. Effective resolution for anionic metabolites.

CE-MS

A powerful tool for the comprehensive analysis of low molecular weight charged metabolites.

Nuclear Magnetic Resonance (NMR) Spectroscopy

Our NMR platform provides a wide range of information on both the identity and quantity of many metabolites in parallel.

  • Applications: measurement of fatty acids, bovine milk analysis, quantitative metabolomic analysis.
  • Pros: unbiased, non-destructive, minimal sample preparation requirements, comprehensive.
  • Cons: low sensitivity of NMR instruments to insufficient sample concentrations, expensive, do not support analysis of higher molecular weight molecules, nuclei without magnetic moments, or molecules with ionic states

Fourier-Transform Infrared Spectroscopy (FT-IR)

FT-IR spectroscopy is a widely used vibrational spectroscopy technique. BioVenic has established an advanced FT-IR platform that can provide a chemical fingerprint of your animal nutritional samples (either solid, liquid, or gaseous).

  • Applications: detecting milk compositions (fatty acids, BHB, acetone and citrate).
  • Pros: inexpensive, high-throughput, non-destructive, not limited by the physical state of the sample.
  • Cons: for analysis in the infrared region only, unsuitable for analyzing watery samples, low sensitivity in quantitative analysis.

Raman Spectroscopy

Our Raman spectroscopy platform is a qualitative and quantitative method capable of providing information such as chemical structure, phase and polymorphism, contamination and impurity of animal nutritional metabolites. Surface-enhanced Raman spectroscopy (SERS), based on the molecular "fingerprint" of the Raman spectrum and the enormous signal enhancement (down to the single molecule level) provided by plasmonic nanomaterials, has proven to be a novel and powerful tool for metabolite detection.

  • Applications: trace detection of urine metabolites, urea in blood or urine, creatinine, lactic acid, and more.
  • Pros: non-destructive, ultra-sensitive, label-free, rapid, specific.
  • Cons: requires intimate contact between the enhancing surface and the analyte, limited selectivity of substrates for a given analyte, limited reusability of substrates, problems with homogeneity and reproducibility, etc.

Metabolomics

Metabolomics generally focuses on small molecules with a molecular weight of less than 1000. It has unparalleled value in measuring the metabolic status of animals, early detection of nutritional disorders in animals and biomarker discovery. With our highly efficient analytical platforms, we are committed to providing you with cutting-edge solutions in animal metabolomics. Visit Omics Solution in Animal Nutrition and Metabolism for more information.

  • Metabolomics Platforms

We provide mass spectrometry (including GC-MS, LC-MS, IC-MS, and CE-MS) and nuclear magnetic resonance (NMR) platforms for animal nutritional metabolomics analysis and related data analysis.

  • Metabolomics Investigating Strategies

Untargeted Metabolomics

With self-built databases or public databases, BioVenic is able to perform qualitative or relative quantitative metabolite detection in various sample types.

  • Applications: biomarker discovery, nutrient effect study, etc.
  • Pros: high throughput, high resolution, wide coverage, no need for internal standards.
  • Cons: only semi-quantitative, difficulty in interpreting results.

Quantitative Metabolomics

By using stable isotopically labeled (SIL) internal standards, we can perform targeted quantification of typical animal nutrition metabolites including amino acids, fatty acids, bile acids, carbohydrates, vitamins, etc.

  • Applications: validation of differential metabolites, experiments with specific research purposes, etc.
  • Pros: reduced false positives, more precise, quantifiable results.
  • Cons: limited to known standard metabolites.

Applications

  • To assess of the health status in animals

By analyzing metabolites, we can evaluate the adequacy of feed nutrition, and to see if it meets the need of the animal.

  • To monitor metabolic disorders

Abnormalities in certain indicators can assist in the diagnosis and treatment of nutritional and metabolic diseases.

  • To develop novel feed or new nutritional strategies

Different forms of nutrients have different bioavailability. Metabolic analysis helps to evaluate the efficiency, safety, and limitation inclusion level of nutrients in feed.

  • To delve the mechanism of certain nutrients

Metabolites act as indicators of the biochemical reactions happened in animals' bodies. Analyzing of nutritional metabolites shows the interactions between nutrients and animals.

  • To discover new nutritional biomarkers

Metabolomic methods and comparative experiments enable the discovery of novel biomarkers in animal nutritional disorders, nutritional deficiencies or excesses, which may be potential targets for the development of clinical diagnosis and treatment.

Animal Nutrition and Metabolism Analysis Workflow

We are able to scrutinize your project needs and select the right assay for you. We have established an assay platform for a wide range of metabolites through a wide range of resources to facilitate your research through a standardized, high-standard assay process. Our one-stop service process can meet your needs for multiple metabolite assays, saving you time and effort.

Animal nutrition and metabolism analysis workflow. (BioVenic Original)

Our Advantages on Animal Nutrition and Metabolism Analysis

  • Our multiple platforms, services and products can be used to analyze nutritional metabolites from different species and sample types, from individual metabolites to metabolomics.
  • Our one-stop service is designed to provide you with reliable technical support, from sample selection and test method selection to test result interpretation, to reduce your worries.
  • We have established extensive collaborations with internal and external technology platforms and are able to provide assay development services to solve your experimental challenges. Other solutions which are not mentioned on the page, such as ion selective electrode (ISE) and capillary electrophoresis (CE), are also available in BioVenic.

In BioVenic, we not only provide with you guaranteed animal nutrition and metabolism analysis services and products, but also offer innovate solutions. Our solutions cover the entire process of animal nutrition and metabolism analysis from sample collection, method development, experimental procedures, data analysis to biological elaboration. Our enthusiastic team will try best to facilitate your project with a wide range of resources to support your animal nutrition and metabolism research. Please feel free to contact us.

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