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Animal Induced Pluripotent Stem Cell Reprogramming
Animal adult cells can be reprogrammed to generate induced pluripotent stem cells (iPSCs), which have characteristics very similar to those of animal embryonic stem cells. This provides another valuable source of pluripotent stem cells for veterinary drug development, cell therapy development and basic cell biology research. BioVenic offers one-stop customized services from reprogramming to iPSC proliferation, validation and differentiation, especially for complex reprogramming technologies. With our state-of-the-art comprehensive animal stem cell technology platform, we offer customers a variety of reprogramming solutions with tightly optimized workflows.
Fig.1 One approach involves employing self-renewal factors to reprogram fibroblasts or blastocysts into iPSCs or ESCs.1,2
Animal iPSC Reprogramming Solutions
BioVenic focuses on the generation of animal iPSCs by inducing the efficient expression of four reprogramming factors, namely OCT-4, SOX-2, KLF-4 and c-MYC. BioVenic develops a variety of induced reprogramming technologies to achieve the development, design and implementation of personalized solutions for customers.
Vector-induced Reprogramming
BioVenic offers personalized services for the reprogramming of adult cells using a variety of vectors. To ensure continuous expression of the introduced exogenous genes in the recipient cells and to guarantee the success of the induction, we use an efficient reprogramming vector solution. This solution includes various types such as viral and non-viral vectors, as well as integrating and non-integrating vector types. Our service can achieve the excision of exogenous genes from the iPSC genome after induction based on the personalized needs of customers. This process results in iPSCs without exogenous gene integration, leading to high efficiency, non-tumorigenicity, and a reduction in the number of transcription factors required.
- Viral vector
- Plasmid vector
- Transposon
- Episomal vector
Small Molecule-induced Reprogramming
BioVenic uses small molecules and chemicals to induce reprogramming of adult animal cells and combines them with other technologies to increase the efficiency of induction. Our animal stem cell laboratory team conducts in-depth research and analysis of the mechanisms involved in improving reprogramming efficiency and generating iPSCs. We provide customers with detailed and reliable reprogramming solutions that address the root cause.
Protein Molecule-induced Reprogramming
BioVenic's custom animal iPSC reprogramming service facilitates the direct use of key transcription factor proteins for induced reprogramming. We utilize various strategies and procedures, such as the incorporation of signal peptides, to increase the stability of the target protein. This ensures that the protein can function effectively on the recipient cells. Our highly efficient and high-quality solutions utilize protein expression and purification technology to achieve large-scale in vitro production of high-purity protein transcription factors. Our service effectively transports key transcription factor proteins into target cells, ensuring stable and effective reprogramming.
siRNA-induced Reprogramming
BioVenic offers scientific researchers services to design and develop solutions for RNA-induced reprogramming of animal cells. The addition of siRNA has a specific regulatory effect on RNA translation levels. Our approach to siRNA-induced reprogramming aims to increase the efficiency of cell induction. Our solution also targets specific signaling pathways related to cell differentiation and pluripotency maintenance that are affected by siRNA. This approach promotes a deeper understanding of the mechanisms involved in the reprogramming of recipient cells during the induction process.
Summary of Factors Affecting Animal Cell Reprogramming
| Factors | Function |
| Hypoxia | During the culture of animal iPSCs, reducing the oxygen concentration in the culture environment can significantly improve the efficiency of cell reprogramming and maintenance of pluripotency. |
| Heat Treatment | After heat treatment, animal cells can be reprogrammed from a differentiated to an undifferentiated state by continuous activation of the extracellular signal-regulated kinase pathway. |
| Cell Interaction | The interaction between animal cells can induce cell dedifferentiation, proliferation, and participation in tissue regeneration through various pathways, including membrane signaling proteins, thereby initiating reprogramming. |
| Oxidative Stress | When the balance between reactive oxygen species (ROS) and the antioxidant system is disrupted in animal cells, chromosomes and heterochromatin disintegrate and then reassemble. As a result, the cell genome undergoes reorganization, the cell cycle restarts, and the cells enter a state of dedifferentiation and reprogramming. |
Why Choose Us?
Our team of animal stem cell experts is skilled in the technical aspects of cell reprogramming to meet the diverse project needs of researchers.
Our reprogramming solutions have been optimized and improved multiple times to ensure that they are simple, efficient, non-tumorigenic, and effective.
We provide an integrated workflow for the culture, expansion, and verification of animal iPSCs, as well as timely technical support for various experimental issues.
BioVenic focuses on providing reprogramming services for animal iPSCs. This involves combining animal cell transfection, vector construction, protein expression and purification using various technical platforms to provide efficient and reliable feasibility solutions. From design and development to implementation, our solutions are specifically optimized and quality controlled to maintain cell pluripotency and ensure the stability of the experimental process. If you are looking for a partner to develop an animal iPSC, please contact us and we will be happy to learn more about your research plan and provide support.
References
- Image retrieved from Figure 1 "One approach involves employing self-renewal factors to reprogram fibroblasts or blastocysts into iPSCs or ESCs." Zhang et al., 2024, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). Without modification.
- Zhang, Teng et al. "Application Prospect of Induced Pluripotent Stem Cells in Organoids and Cell Therapy." International journal of molecular sciences vol. 25,5 2680. 26 Feb. 2024.