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Animal Induced Pluripotent Stem Cell Characterization
Verification that the animal cell types generated are the target cell types needed by researchers is essential to ensure that subsequent experiments proceed smoothly and that optimal research results are ultimately achieved. Normal pluripotent stem cells should be fully characterized and verified both before and after differentiation to ensure the integrity of the next experimental work. This includes performing pluripotency characterization, immunoassays of cell markers after differentiation, and other necessary assessments. To characterize cell lines as true pluripotent stem cells and to verify the production of somatic cells of different lineages after differentiation, BioVenic performs rigorous evaluations and develops exclusive service plans. These services ensure that the differentiation capacity of the cells remains intact or that differentiation is completed.
Fig.1 Workflow for Phenotypic Analysis of Mammalian iPSCs.1,2
Animal iPSC Characterization Service
BioVenic's characterization of newly generated animal iPSCs focuses on the pluripotency of the cells and the complete silencing of induced exogenous genes.
Gene Level Characterization
BioVenic monitors and analyzes the expression of transcription factors that induce reprogramming by detecting gene expression at the RNA level. At the same time, it characterizes the expression levels of key genes that directly determine cell pluripotency. We have developed a comprehensive platform for cell transcriptome extraction and high-throughput analysis to provide high quality customized services to our customers.
Protein Level Characterization
BioVenic supports the characterization of animal iPSCs by detecting specific pluripotency markers at the protein level using immunocytochemistry. We have accumulated rich project experience in protein detection and immunological labeling, supporting high-throughput project requirements and ensuring service quality and scientific solutions.
Embryoid Formation Test
BioVenic offers personalized solutions to assess iPSC pluripotency by examining the formation of embryoid bodies during in vitro differentiation. When cells exhibit good pluripotency, they can spontaneously differentiate into derivatives of the three germ layers in vitro. This ability is a critical criterion for reliable and comprehensive verification of cell functionality.
Animal iPSC Differentiation Characterization Service
BioVenic offers comprehensive and reliable solutions for the characterization and identification of target cell populations differentiated from animal iPSCs derived from various tissues.
Cell Marker Detection
BioVenic uses immunolabeling detection as a solution to characterize different types of post-differentiated cells. Animal induced pluripotent stem cells (iPSCs) have the ability to differentiate into three germ layers and develop into various somatic cell types. We use markers on the surface of animal tissue cells to detect, distinguish, and verify the generation of specific target cells.
| Cells | Signature Proteins |
| Neurons | α-Synuclein, NCAM, GAP-43, Glutamine synthetase, MASH1, mGluR1α, Nistin, Nicastrin, Pax-5, Synaptophysin. |
| Astrocytes | GFAP, EAAT1, EAAT2, Glutamine synthetase, ALDH1L1. |
| Pancreatic Cells | Glucagon, Insulin, Insulin-1, Pancreatic polypeptide, PDX-1. |
| Cardiomyocytes | α-Actinin, Cardiac troponin, GATA4, GATA6, Myogenin. |
| Hepatocytes | α-Fetoprotein, Albumin, HNF-1α, Tat-SF1. |
Why Choose Us?
Comprehensive Animal Cell-specific Protein Library
BioVenic can accurately characterize cells differentiated from iPSCs due to its detailed knowledge of key proteins in cells from different species and tissues.
One-stop Induced Pluripotent Stem Cell Solution
BioVenic offers customized, comprehensive solutions for animal stem cells, including preparation, culture, maintenance, and differentiation, to effectively address customers' research needs in the field of iPSCs.
Professional Background in Animal Stem Cell Research
BioVenic's team of experts and technicians have a strong academic background in cell and molecular biology. We have expertise in both theoretical knowledge and practical skills.
BioVenic provides high quality customized services to researchers worldwide who require animal iPSC-related cell characterization. We have developed standardized detection and verification experimental techniques for the most critical characterization directions in the field. These include the silencing of transcription factors used for reprogramming, the manifestation of pluripotency, and the characteristics of target somatic cells after differentiation. Please contact us for efficient, reliable and comprehensive cell characterization solutions.
References
- Image retrieved from Figure 1 "Multistep workflow for phenotyping of hiPSCs." Chen et al., 2021, used under [CC BY 4.0] (https://creativecommons.org/licenses/by/4.0/). The original title was changed to "Workflow for Phenotypic Analysis of Mammalian iPSCs."
- Chen, Carol X-Q., et al. "A multistep workflow to evaluate newly generated iPSCs and their ability to generate different cell types." Methods and protocols 4.3 (2021): 50.