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Ovine Alveolar Epithelial Primary Cells-Luciferase

Product Overview

Ovine alveolar epithelial primary cells expressing luciferase are isolated from alveolar tissue in ovine lung. After isolating and culturing these cells, the third generation cells are cryopreserved, demonstrating high viability and activity. Ovine alveolar epithelial primary cell products are utilized in various standard biochemical procedures to characterize cell products and derivatives, as well as to investigate cell-cell interactions. These cell products stably expressed luciferase which transfected with lentivirus, achieving visualization purposes in various related research applications through bioluminescence.

Specifications

Cell Product Type Primary Cells-Luciferase

Species Ovine

Cell Type Epithelial Cells

Source Lung

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium Phosphate Buffered Saline; DMEM with 10% FBS; 1% Antibiotics-Antimycotics

Physical State Frozen cells or live cells in liquid culture medium

Target Information

Ovine epithelial primary cell products stably expressing luciferase achieve bioluminescence by efficiently producing highly stable monomeric luciferase proteins.

Target/Biomarker Luciferase

Target Synonym luciferin 4-monooxygenase [ Photinus pyralis (common eastern firefly) ]; luc; LUC1; PpyLuc1

Gene ID 116160065

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

The product is customized. Please contact info@biovenic.com for more detailed information.

References

Marcinkiewicz, Mariola M., et al. "A novel approach for ovine primary alveolar epithelial type ii cell isolation and culture from fresh and cryopreserved tissue obtained from premature and juvenile animals." PLoS One 11.3 (2016): e0152027.