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Porcine Corneal Epithelial Primary Cells-Luciferase

Product Overview

Luciferase-expressing porcine corneal epithelial primary cells are isolated from porcine cornea, cultured, and harvested at passages 2-3 to ensure the viability and activity of the product. Luciferase-expressing porcine corneal epithelial primary cell products are utilized in various standard biochemical procedures to characterize cell products and derivatives, as well as to investigate cell-cell interactions. These cell products stably express lentivirally transfected luciferase and achieve visualization purposes in various related research applications through bioluminescence.

Specifications

Cell Product Type Primary Cells-Luciferase

Species Swine

Cell Type Epithelial Cells

Source Cornea

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium Phosphate Buffered Saline; 10% DMSO; 50% FBS; 40% Culture Medium

Physical State Frozen cells or live cells in liquid culture medium

Target Information

Porcine epithelial primary cell products stably expressing luciferase achieve bioluminescence by efficiently producing highly stable monomeric luciferase proteins.

Target/Biomarker Luciferase

Target Synonym luciferin 4-monooxygenase [ Photinus pyralis (common eastern firefly) ]; luc; LUC1; PpyLuc1

Gene ID 116160065

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

The product is customized. Please contact info@biovenic.com for more detailed information.

References

Wenzel, Daniel A., et al. "Effect of elevated irrigation bottle height during cataract surgery on corneal endothelial cells in porcine eyes." BMC ophthalmology 23.1 (2023): 211.