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Porcine Retinal Microvascular Endothelial Primary Cells

Product Overview

Porcine retinal microvascular endothelial primary cells are isolated and cultured from porcine retinal microvascular tissue. These cells are obtained from the third generation after isolation and culture, and they exhibit high vitality and activity. Porcine retinal microvascular endothelial primary cell products are utilized in various standard biochemical procedures, including RT-PCR, Western blotting, immunoprecipitation, and flow cytometry, for scientific research purposes. They are also employed for characterizing cell products and derivatives necessary for scientific research.

Specifications

Cell Product Type Primary Cells

Species Swine

Cell Type Endothelial Cells

Source Retinal Tissue

Growth Information Adherent; 37°C; 5%CO₂

Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)

Formulation and Cell Culture Medium Phosphate Buffered Saline; 10% DMSO; 50% FBS; 40% Culture Medium

Physical State Frozen cells or live cells in liquid culture medium

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

These cells were precoated with PECAM-1 (CD31) antibody as secondary antibody. The PECAM-1 antibody, conjugated to a paramagnetic bead-coupled antibody, was utilized to identify the endothelial cell-specific antigen, platelet/endothelial cell adhesion molecule (PECAM), and isolate pure endothelial cells from samples contaminated with non-endothelial cells.