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Recombinant Canine 5'-Nucleotidase (NT5E), C-His

Product Overview

BioVenic's Recombinant Canine 5'-Nucleotidase (NT5E), C-His is a recombinant protein expressed from HEK293 Cells. Its predicted molecular weight is 59.8 kDa. The purity is>95% (SDS-PAGE). The endotoxin level is <1 EU/μg (LAL).

Specifications

Type Recombinant Protein

Species Canine

Expression System HEK293 Cells

Biological Activity Active

Purity >95% (SDS-PAGE)

Endotoxin <1 EU/μg (LAL)

Predicted Molecular Weight 59.8 kDa

Molecular Weight 65 kDa

Physical State Lyophilized

Formulation 20 mM Tris, 120 mM NaCl, pH 7.5 with trehalose.

Target Information

5'-Nucleotidase ecto is encoded by the NT5E gene in dogs. It serves as one of the cell surface markers and contains binding sites for transcription factors such as AP-2, SMAD proteins, SP-1, as well as elements responsive to cAMP, which are present in cAMP promoter regions.

Protein Canine 5'-Nucleotidase (NT5E)

Protein Synonym 5'-nucleotidase ecto, 5'-nucleotidase, 5'-nucleotidase, ecto (CD73)

Gene ID 474984

EC No. 3.1.3.5

Shipping and Storage

This product is shipped with dry ice. Avoid repeated freezing and thawing. Store for 12 months at -20°C or -80°C.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Aliquote the reconstituted solution to minimise freeze-thaw cycles.

References

Shahsavari, A. et al. Pluripotency and immunomodulatory signatures of canine induced pluripotent stem cell-derived mesenchymal stromal cells are similar to harvested mesenchymal stromal cells. Scientific reports. 2021, 11: 3486.