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Porcine Alveolar Epithelial Primary Cells

Cat. No.CC11L85

Product TypeAnimal Cells and Cell Lines

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Product Overview

Porcine alveolar epithelial primary cells are isolated from porcine alveolar tissue. After isolating and culturing these cells, the third-generation cells are cryopreserved, demonstrating high viability and activity. Porcine alveolar epithelial primary cell products are utilized in various standard biochemical procedures, such as PCR, Western blot analysis, immunoprecipitation, and flow cytometry, for scientific research purposes. They are also used to characterize cellular products and derivatives required for scientific research, as well as to explore cell-cell interactions.

Specifications

Cell Product Type Primary Cells
Species Swine
Cell Type Epithelial Cells
Source Lung
Growth Information Adherent; 37°C; 5%CO2
Quality Control Mycoplasma (-); Bacteria (-); Yeast (-); Fungi (-)
Formulation and Cell Culture Medium Phosphate Buffered Saline; 10% DMSO; 50% FBS; 40% Culture Medium
Physical State Frozen cells or live cells in liquid culture medium

Shipping and Storage

This product is shipped with dry ice. It's recommended to store the product with liquid nitrogen and never in a -20 °C or -80 °C refrigerator. Avoid repeated freezing and thawing of the product.

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

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User Note

  1. These cells were precoated with EpCAM-1 (CD326) antibody as secondary antibody. The EpCAM-1 antibody, conjugated to a paramagnetic bead-coupled antibody, was utilized to identify the epithelial cell-specific antigen, epithelial cell adhesion molecule (EpCAM), and isolate pure epithelial cells from samples contaminated with non-epithelial cells.

References

  1. Raymond, B. B. A., et al. "Mycoplasma hyopneumoniae resides intracellularly within porcine epithelial cells." Scientific reports 8.1 (2018): 17697.
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