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Recombinant Canine Epididymal Secretory Glutathione Peroxidase (GPX5), N-His-SUMO and C-Myc

Cat. No.AP9C594

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Canine Epididymal Secretory Glutathione Peroxidase (GPX5), N-His-SUMO and C-Myc is a recombinant protein expressed from E.coli. Its predicted molecular weight is 42.6 kDa. The purity is>90% (SDS-PAGE).

Specifications

Type Recombinant Protein
Species Canine
Expression System E.coli
Purity >90% (SDS-PAGE)
Predicted Molecular Weight 42.6 kDa
Physical State Lyophilized
Formulation The buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Target Information

Swine epididymal secretory glutathione peroxidase (GPX5), also known as phospholipid hydroperoxide glutathione peroxidase, is an important antioxidant enzyme in the epididymis of pigs. GPX5 is primarily involved in protecting sperm cells from oxidative damage by reducing phospholipid hydroperoxides to alcohols, thereby maintaining the integrity of sperm cell membranes. This protein plays a crucial role in sperm maturation and function, as oxidative stress can impair sperm motility and fertility. In pigs, GPX5 contributes to the overall reproductive health and the success of fertilization by ensuring the sperm's ability to swim and penetrate the egg. It has been identified as a marker for boar sperm quality, with higher amounts in seminal plasma correlating with lower sperm quality parameters such as membrane integrity and motility.

Protein Canine Epididymal Secretory Glutathione Peroxidase (GPX5)
Protein Synonym GPX5Epididymal secretory glutathione peroxidase; EC 1.11.1.9; Epididymis-specific glutathione peroxidase-like protein; EGLP; Glutathione peroxidase 5; GPx-5; GSHPx-5
Gene ID 396920
UniProt ID O18994

Shipping and Storage

This product is shipped with dry ice. It is recommended to aliquot as needed and store at -80°C upon receipt. Reconstituted protein solution can be stored at 4°C for 1 week, at < -80°C for 12 months. Avoid repeated freezing and thawing.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Aliquot the reconstituted solution to minimise freeze-thaw cycles.
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