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Bovine Aspartate Aminotransferase, Cytoplasmic (GOT1) ELISA Kit-Sandwich

Name: Bovine Aspartate Aminotransferase, Cytoplasmic (GOT1) ELISA Kit-Sandwich
SKU: EK12F335
Rating: 5 (1 reviews)

Cat. No.EK12F335

Product TypeAnimal Immunoassay Kits

Size

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Product Overview

BioVenic Bovine Aspartate Aminotransferase, Cytoplasmic (GOT1) ELISA Kit-Sandwich is designed for the quantitative determination of Bovine Aspartate Aminotransferase, Cytoplasmic (GOT1) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich

Specificity The assay kit is specific for Bovine GOT1.

Target Species Bovine

Species Reactivity Bovine

Detection Range 0.156-10 ng/mL

Sensitivity 0.055 ng/mL

Recovery 80%-102%

Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%

Assay Time Around 270 min

Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids.

Target Information

Glutamic-oxaloacetic transaminase 1 (GOT1), also known as aspartate aminotransferase, cytoplasmic, is encoded by the GOT1 gene in cattle. GOT1 is a pyridoxal phosphate-dependent enzyme that exists in both cytoplasmic and mitochondrial forms. It plays a role in amino acid metabolism, as well as the urea and tricarboxylic acid cycles. The enzyme catalyzes the reversible transfer of an amino group between aspartate and α-ketoglutarate to form oxaloacetate and glutamate. It is a gene implicated in protection from cellular stress.

Target/Biomarker Bovine GOT1

Target Synonym glutamic-oxaloacetic transaminase 1; cCAT; cAspAT; aspartate aminotransferase, cytoplasmic; aspartate aminotransferase 1; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1, soluble; transaminase A

Gene ID 281206

UniProt ID P33097

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

Dikmen, S. et al. Genome-Wide Association Mapping for Identification of Quantitative Trait Loci for Rectal Temperature during Heat Stress in Holstein Cattle. PloS one. 2013, 8: e69202.