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Recombinant Bovine Nitric Oxide Synthase, Endothelial (NOS3), N-His
Product Overview
BioVenic's Recombinant Bovine Nitric Oxide Synthase, Endothelial (NOS3), N-His is a recombinant protein expressed from E.coli expression system. Its predicted molecular weight is 24.0 kDa. The purity is greater than 95% (SDS-PAGE). The endotoxin level is <1 EU/μg (LAL). It can be used as a positive control or as an immunogen.
Specifications
Target Information
Nitric Oxide Synthase, Endothelial is an enzyme expressed in the endothelium (lining of blood vessels) that synthesizes nitric oxide (NO), a signaling molecule involved in vasodilation, blood pressure regulation, and platelet inhibition. It is encoded by the NOS3 gene in cattle. It is also known as eNOS. It belongs to a unique family of proteins that catalyzed a 5-electron oxidation of a guanidino nitrogen atom of L-arginine in the presence of molecular oxygen and NADPH, imultaneously forming NO and stoichiometric amounts of L-citrulline.
Shipping and Storage
This product is shipped with dry ice. Avoid repeated freezing and thawing. Store for 4 weeks at 2-8°C, for 12 months at -80°C.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Aliquote the reconstituted solution to minimise freeze-thaw cycles.