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Recombinant Porcine Stromal Cell Derived Factor 1 (SDF1), His-TRxA
Product Overview
BioVenic's Recombinant Porcine Stromal Cell Derived Factor 1 (SDF1), His-TRxA is a recombinant protein expressed from E.coli expression system. Its predicted molecular weight is 31.4 kDa. The purity is>90% (SDS-PAGE). The endotoxin level is <1 EU/μg (LAL). It can be used as a positive control or as an immunogen.
Specifications
Target Information
C-X-C motif chemokine ligand 12 is encoded by the CXCL12 gene in pigs. CXCL12 is abundant in reproductive tissues. Research has shown that 10 ng/mL CXCL12 can induce denuded oocytes to extrude PB1, suggesting that a direct CXCL12-CXCR4 interaction occurs during porcine IVM.
Shipping and Storage
This product is shipped with dry ice. Avoid repeated freezing and thawing. Store for 4 weeks at 2-8°C, for 12 months at -80°C.
Documents
The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.
User Note
- Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Aliquote the reconstituted solution to minimise freeze-thaw cycles.
References
- Liu, X. et al. Maternal Cytokines CXCL12, VEGFA, and WNT5A Promote Porcine Oocyte Maturation via MAPK Activation and Canonical WNT Inhibition. Frontiers in cell and developmental biology. 2020, 8: 578.