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Recombinant Salmonella typhimurium UDP-N-Acetylmuramoyl-L-Alanyl-D-Glutamate--2,6-Diaminopimelate Ligase (murE), N-His

Cat. No.VG9C144

Product TypeVeterinary Antigens

Size

Product Overview

BioVenic's Recombinant Salmonella typhimurium UDP-N-Acetylmuramoyl-L-Alanyl-D-Glutamate--2,6-Diaminopimelate Ligase (murE), N-His is a recombinant protein expressed from E.coli. Its predicted molecular weight is 33.6 kDa. The purity is>90% (SDS-PAGE).

Specifications

Type Recombinant Protein
Species Bacterium
Expression System E.coli
Purity >90% (SDS-PAGE)
Predicted Molecular Weight 33.6 kDa
Physical State Lyophilized
Formulation The buffer before lyophilization is a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Target Information

In the veterinary field, the function of Salmonella typhimurium UDP-N-Acetylmuramoyl-L-Alanyl-D-Glutamate--2,6-Diaminopimelate Ligase (murE) is primarily involved in a key step of bacterial cell wall synthesis. MurE is a cytoplasmic enzyme that catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG), forming UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-diaminopimeloyl-L-alanyl-D-glutamate pentapeptide, which is essential for bacterial growth and division. Therefore, MurE is a potential target for the development of antibiotics against Salmonella infections, playing a significant role in disease prevention and treatment in the veterinary field.

Protein Salmonella typhimurium UDP-N-Acetylmuramoyl-L-Alanyl-D-Glutamate--2,6-Diaminopimelate Ligase (murE)
Protein Synonym Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UniProt ID Q8ZRU7

Shipping and Storage

This product is shipped with dry ice. Avoid repeated freezing and thawing. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.Aliquote the reconstituted solution to minimise freeze-thaw cycles.
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