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Porcine Isocitrate Dehydrogenase (IDH) ELISA Kit-Sandwich

Name: Porcine Isocitrate Dehydrogenase (IDH) ELISA Kit-Sandwich
SKU: EK12F558
Rating: 5 (1 reviews)

Cat. No.EK12F558

Product TypeAnimal Immunoassay Kits

Size

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Product Overview

BioVenic Porcine Isocitrate Dehydrogenase(IDH) ELISA Kit-Sandwich is designed for the quantitative determination of Porcine Isocitrate Dehydrogenase(IDH) in serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids using a Sandwich ELISA method. For research use only.

Specifications

Assay Type ELISA-Sandwich

Specificity The assay kit is specific for Porcine IDH.

Target Species Swine

Species Reactivity Swine

Reproducibility Intra-Assay: CV < 10%; Inter-Assay: CV < 10%

Assay Time Around 270 min

Sample Requirement Serum, plasma, tissue homogenate, cell culture supernatant, cell extract, and other biological fluids.

Target Information

Isocitrate dehydrogenase (IDH) (EC 1.1.1.42) and (EC 1.1.1.41) is an enzyme that catalyses the oxidative decarboxylation of isocitrate to produce alpha-ketoglutarate (α-ketoglutarate) and CO2. This is a two-step process involving the oxidation of isocitrate (a secondary alcohol) to oxalosuccinate (a ketone), followed by the decarboxylation of the beta-carboxyl group to the ketone to produce alpha-ketoglutarate. IDH1 is a key NADPH generator in cells, necessary for the regeneration of GSH from GSSH. Research has shown that silencing IDH1 leads to increased levels of ROS, which induce granulosa cell apoptosis.

Target/Biomarker Porcine IDH

Target Synonym Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; IDH1; 1.1.1.42

Shipping and Storage

This product is shipped with gel ice packs. It is recommended to store at 2-8 °C (Up to 6 months).

Documents

Datasheet

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

References

Meng, L. et al. Transcriptome Analysis of Porcine Granulosa Cells in Healthy and Atretic Follicles: Role of Steroidogenesis and Oxidative Stress. Antioxidants (Basel, Switzerland). 2020, 10.