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Recombinant Bovine Interstitial Collagenase (MMP1), N-His

Cat. No.AP9C485

Product TypeAnimal Proteins

Size

Product Overview

BioVenic's Recombinant Bovine Interstitial Collagenase (MMP1), N-His is a recombinant protein expressed from E.coli. Its predicted molecular weight is 55.1 kDa. The purity is>90% (SDS-PAGE).

Specifications

Type Recombinant Protein
Species Bovine
Expression System E.coli
Purity >90% (SDS-PAGE)
Endotoxin < 1 EU/µg
Predicted Molecular Weight 55.1 kDa
Molecular Weight 55.0 kDa
Physical State Lyophilized
Formulation The buffer before lyophilization is 100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Target Information

Bovine interstitial collagenase (MMP1) is an enzyme found in cattle that is responsible for the breakdown of the extracellular matrix, specifically targeting fibrillar collagens such as types I, II, and III. This protein is crucial in physiological processes like tissue remodeling and wound healing, where it cleaves collagen within the helical domain. It's also involved in pathological conditions such as arthritis and tumor invasion. In the medical field, understanding and utilizing Bovine interstitial collagenase can aid in the development of treatments for diseases associated with abnormal collagen metabolism.

Protein Bovine Interstitial Collagenase (MMP1)
Protein Synonym CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase
Gene ID 112441463
UniProt ID P28053

Shipping and Storage

This product is shipped with dry ice. It is recommended to aliquot as needed and store at -80°C upon receipt. Reconstituted protein solution can be stored at 4°C for 1 week, at < -80°C for 12 months. Avoid repeated freezing and thawing.

Documents

COA

To request a Certificate of Analysis, please enter the Lot No. in the search box. Note: Certificate of Analysis not available for kits.

The product is for research use only.
Not for commercial, prophylactic, diagnostic, or therapeutic applications.

User Note

  1. Always centrifuge tubes before opening. Avoid mixing by vortexing or pipetting. Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Aliquot the reconstituted solution to minimise freeze-thaw cycles.

References

  1. Daraban Bocaneti, Florentina. et al. Expression of collagenases (matrix metalloproteinase-1, -8, -13) and tissue inhibitor of metalloproteinase-3 (TIMP-3) in naturally occurring Sheep cutaneous fibropapillomas.Frontiers in veterinary science. 2023,.9 1072672: 12
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